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Literature summary for 3.4.21.5 extracted from

  • Backes, B.J.; Harris, J.L.; Leonetti, F.; Craik, C.S.; Ellman, J.A.
    Synthesis of positional-scanning libraries of fluorogenic peptide substrates to define the extended substrate specificity of plasmin and thrombin (2000), Nat. Biotechnol., 18, 187-193.
    View publication on PubMed
Search for more literature for 3.4.21.5

Inhibitors

Inhibitors Comment Organism Structure
D-Phe-Pro-Arg-chloromethylketone
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 Ac-Nle-Thr-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 25°C Homo sapiens
0.03
-
 Ac-Val-Thr-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 25°C Homo sapiens
0.067
-
 Ac-Nle-Thr-Leu-Arg-7-amido-4-methylcoumarin pH 8.0, 25°C Homo sapiens
0.102
-
 Ac-Leu-Gly-Val-Arg-7-amido-4-methylcoumarin pH 8.0, 25°C Homo sapiens
0.181
-
 Ac-Gly-Gly-Val-Arg-7-amido-4-methylcoumarin pH 8.0, 25°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme is important in blood coagulation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
selective cleavage of Arg-/-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B reaction mechanism, substrate binding, preference at positions P1-P4, molecular modeling of enzyme-substrate complex Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-Gly-Gly-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ac-Gly-Gly-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
Ac-Leu-Gly-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ac-Leu-Gly-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
Ac-Nle-Thr-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ac-Nle-Thr-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
Ac-Nle-Thr-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ac-Nle-Thr-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
Ac-Val-Thr-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ac-Val-Thr-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
factor V (1018) + H2O cleavage site is LSPR Homo sapiens ?
-
 ?
factor V (1545) + H2O cleavage site is WYLR Homo sapiens ?
-
 ?
factor V (709) + H2O
-
 Homo sapiens ?
-
 ?
factor VII (1689) + H2O clevage site is QSPR Homo sapiens ?
-
 ?
factor VII (372) + H2O cleavage site is IQIR Homo sapiens ?
-
 ?
factor VII (740) + H2O
-
 Homo sapiens ?
-
 ?
factor XI + H2O
-
 Homo sapiens ?
-
 ?
factor XIII + H2O
-
 Homo sapiens ?
-
 ?
fibrinogen A a + H2O
-
 Homo sapiens ?
-
 ?
fibrinogen B b + H2O
-
 Homo sapiens ?
-
 ?
additional information substrate specificity, peptide substrate library scanning Homo sapiens ?
-
 ?
additional information enzyme is important in blood coagulation Homo sapiens ?
-
 ?
protein C + H2O
-
 Homo sapiens ?
-
 ?
transmembrane receptor PAR1 + H2O
-
 Homo sapiens ?
-
 ?
UpA + H2O
-
 Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 Ac-Gly-Gly-Val-Arg-7-amido-4-methylcoumarin pH 8.0, 25°C Homo sapiens
5.8
-
 Ac-Nle-Thr-Leu-Arg-7-amido-4-methylcoumarin pH 8.0, 25°C Homo sapiens
15.6
-
 Ac-Leu-Gly-Val-Arg-7-amido-4-methylcoumarin pH 8.0, 25°C Homo sapiens
30.8
-
 Ac-Val-Thr-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 25°C Homo sapiens
45
-
 Ac-Nle-Thr-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 25°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens