3.4.21.5: thrombin

This is an abbreviated version, for detailed information about thrombin, go to the full flat file.

Reaction

selective cleavage of Arg-/-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B =

Synonyms

activated factor II, alpha-thrombin, alphaTh, beta-thrombin, blood-coagulation factor II, activated, blood-coagulation factor IIa, clotting factor IIa, EC 3.4.4.13, factor IIa, fibrinogenase, thrombase, thrombin, E, thrombin-C, thrombofort, topical, tropostasin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.5 thrombin

Cloned

Cloned on EC 3.4.21.5 - thrombin

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination of the natural mutant gene
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engineering of a prothrombin cDNA coding for a secreted form of prethrombin-2, production in a mammalian expression system using DXB11 cells, a mutant strain of CHO cells in which the dihydrofolate reductase gene has been deleted and an expression vector carrying the dihydrofolate reductase cDNA
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expressed in Mus musculus
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expression of enzyme mutants in BHK cells
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expression of wild-type and mutant enzymes in baby hamster kidney cells
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expression of wild-type and mutant enzymes in CHO cells
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expression of wild-type and mutant enzymes in EA.hy926 cells, co-expression with activated protein C
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recombinant expression of wild-type and mutant enzymes in baby hamster kidney cells, only mutants W215M and W215T, and also the wild-type enzyme are expressed in Escherichia coli strain BL21(DE3)
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wild type and mutant enzymes are expressed as prethrombin-2 zymogens in baby hamster kidney cells using the pNUT-PL2 expression/purification vector system
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