3.2.1.B26: Sulfolobus solfataricus beta-glycosidase
This is an abbreviated version!
For detailed information about Sulfolobus solfataricus beta-glycosidase, go to the full flat file.
Word Map on EC 3.2.1.B26
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3.2.1.B26
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transglycosylation
-
synthesis
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beta-d-galactoside
-
beta-glucosides
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amygdalin
-
glycone
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3.2.1.21
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transgalactosylation
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prunasin
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beta-d-fucoside
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food industry
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analysis
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nutrition
- 3.2.1.B26
-
transglycosylation
- synthesis
- beta-d-galactoside
- beta-glucosides
- amygdalin
-
glycone
-
3.2.1.21
-
transgalactosylation
- prunasin
- beta-d-fucoside
- food industry
- analysis
- nutrition
Reaction
Wide substrate specificity, active on aryl-beta-D-galactose, -alpha-L-fucose, -beta-D-glucose and -beta-D-xylose and on di- and oligosaccharides. D-Glucose dimers are hydrolysed in the order of decreasing efficiency: beta-(1,3), beta-(1,4), beta-(1,6). Exo-acting enzyme with a preference for cellotetraose. =
Synonyms
beta-D-glycosidase, beta-glucosidase, beta-Gly, beta-glycosidase, Bgl, bgly, EcSbgly, GH1 beta-glycosidase, LACS, Sbeta-gly, Sbetagly, Ss-beta-Gly, Ssbeta-Glc1, Ssbeta-Gly, SsbetaG, SsbetaGlc1, SsbetaGly, SsGH1, sso1353, SSO3019
ECTree
3.2.1.B26 Sulfolobus solfataricus beta-glycosidaseAdvanced search results
results (
results (KCat KM Value
KCat KM Value on EC 3.2.1.B26 - Sulfolobus solfataricus beta-glycosidase
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kcat/KM VALUE [1/mMs-1] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
1617
2,4-dinitrophenyl beta-D-glucopyranoside
-
pH 6.5, 65°C, wild-type enzyme
0.034
methyl beta-D-galactoside
release of methanol, pH 7.5, 80°C
313.6
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75°C, immobilized enzyme
337
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75°C, soluble enzyme, enzyme immobilized on chitosan activated with glutaraldehyde
412
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75°C, soluble enzyme
439
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75°C, soluble enzyme
712
2-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, native enzyme
844
2-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
1200
2-nitrophenyl beta-D-galactoside
release of 2-nitrophenol, pH 7.5, 80°C
18.5
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 6.0, 65°C
23.8
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 5.0, 65°C
45
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 6.5, 65°C, presence of 2 M formate
55
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 3.0, 65°C
65.9
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 4.0, 65°C
533
2-nitrophenyl beta-D-glucopyranoside
-
pH 6.5, 65°C, wild-type enzyme
533
2-nitrophenyl beta-D-glucopyranoside
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wild-type, pH 6.5, 65°C
850
2-nitrophenyl beta-D-glucopyranoside
-
wild-type, pH 6.5, 65°C, presence of 2 M formate
53
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80°C, mutant enzyme E432C
76
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80°C, mutant enzyme W433C
4000
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80°C, mutant enzyme M439C
7300
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80°C, wild-type enzyme
0.053
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80°C, mutant enzyme E432C
0.068
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 45°C, mutant enzyme E432C
0.076
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80°C, mutant enzyme W433C
0.506
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 45°C, mutant enzyme W433C
2.5
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C
7.3
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80°C, wild-type enzyme
42.6
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 45°C, wild-type enzyme
53
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C
7300
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme
6.3
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80°C, mutant enzyme W433C
11
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80°C, mutant enzyme E432C
1130
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80°C, mutant enzyme M439C
1490
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80°C, wild-type enzyme
0.0063
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80°C, mutant enzyme W433C
0.011
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80°C, mutant enzyme E432C
0.015
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45°C, mutant enzyme W433C
0.058
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45°C, mutant enzyme E432C
0.6
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C
1.49
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80°C, wild-type enzyme
11
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C
12.7
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45°C, wild-type enzyme
1490
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme
0.0006
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 80°C, wild-type enzyme
0.001
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 45°C, mutant enzyme E432C
0.0013
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 45°C, mutant enzyme W433C
0.06
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 45°C, wild-type enzyme
15
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80°C, mutant enzyme E432C
20
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80°C, mutant enzyme W433C
2900
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80°C, mutant enzyme M439C
2900
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80°C, wild-type enzyme
0.015
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80°C, mutant enzyme E432C
0.02
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80°C, mutant enzyme W433C
0.036
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45°C, mutant enzyme E432C
0.123
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45°C, mutant enzyme W433C
2.9
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80°C, wild-type enzyme
6.3
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C
15
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C
29.8
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45°C, wild-type enzyme
2900
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme
0.6
4-methylumbelliferyl beta-D-glucuronic acid
-
pH 6.5, 80°C, wild-type enzyme
0.92
4-methylumbelliferyl beta-D-glucuronic acid
-
pH 6.5, 80°C, mutant enzyme M439C
0.6
4-methylumbelliferyl beta-D-glucuronide
pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme
5.1
4-methylumbelliferyl beta-D-glucuronide
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C
3.2
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80°C, mutant enzyme E432C
5.1
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80°C, mutant enzyme W433C
50
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80°C, wild-type enzyme
53
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80°C, mutant enzyme M439C
0.001
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 45°C, mutant enzyme E432C
0.0032
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80°C, mutant enzyme E432C
0.005
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 45°C, mutant enzyme W433C
0.0051
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80°C, mutant enzyme W433C
0.05
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80°C, wild-type enzyme
0.11
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 45°C, wild-type enzyme
3.2
4-methylumbelliferyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C
50
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80°C, wild-type enzyme
76
4-methylumbelliferyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C
2.2
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80°C, mutant enzyme E432C
2.5
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80°C, mutant enzyme W433C
30
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80°C, wild-type enzyme
136
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80°C, mutant enzyme M439C
0.002
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 45°C, mutant enzyme E432C
0.0022
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80°C, mutant enzyme E432C
0.0025
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80°C, mutant enzyme W433C
0.003
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 45°C, mutant enzyme W433C
0.03
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80°C, wild-type enzyme
0.77
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 45°C, wild-type enzyme
2.2
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C
20
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C
30
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme
941
4-nitrophenyl beta-D-fucopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
1151
4-nitrophenyl beta-D-fucopyranoside
-
pH and temperature not specified in the publication, native enzyme
0.068
4-nitrophenyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C
0.506
4-nitrophenyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C
42.6
4-nitrophenyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme
370
4-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
576
4-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, native enzyme
0.015
4-nitrophenyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C
0.058
4-nitrophenyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C
12.7
4-nitrophenyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme
1058
4-nitrophenyl beta-D-glucopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
1633
4-nitrophenyl beta-D-glucopyranoside
-
pH and temperature not specified in the publication, native enzyme
0.036
4-nitrophenyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C
0.123
4-nitrophenyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C
29.8
4-nitrophenyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme
0.001
4-nitrophenyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C
0.005
4-nitrophenyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C
0.11
4-nitrophenyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme
0.002
4-nitrophenyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C
0.003
4-nitrophenyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C
0.77
4-nitrophenyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme
1764
4-nitrophenyl-beta-D-glucopyranoside
-
pH 6.0, 60°C, 1500 bar
3356
4-nitrophenyl-beta-D-glucopyranoside
-
pH 6.0, 60°C, 1000 bar
11130
4-nitrophenyl-beta-D-glucopyranoside
-
pH 6.0, 60°C, 500 bar
19.2
cellobiose
-
pH and temperature not specified in the publication, soluble enzyme
25
cellobiose
-
pH and temperature not specified in the publication, recombinant enzyme
29
cellobiose
-
pH and temperature not specified in the publication, native enzyme
33.7
cellobiose
-
pH and temperature not specified in the publication, immobilized enzyme
37.3
cellobiose
-
pH 7.0, 75°C, enzyme immobilized on chitosan activated with glutaraldehyde
697
ginsenoside Rb1
mutant enzyme W361F, at pH 5.5 and 95°C
407
laminaribiose
-
pH and temperature not specified in the publication, native enzyme
410
laminaribiose
-
pH and temperature not specified in the publication, recombinant enzyme
additional information
additional information
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the efficiency (kcat/KM) increases with temperature and reaches its maximal efficiency at 65°C. Reactions with 4-nitrophenyl-beta-D-glucoside and 2-nitrophenyl-beta-D-glucoside show a biphasic behavior in LineweaverBurk plots, suggesting that transglycosylation reactions occur at higher substrate concentrations. Kinetic constants obtained at lower or higher substrate concentrations are calculated
-
additional information
additional information
-
kinetic constants for transglycosylation reactions
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