Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
4-nitrophenyl beta-D-fucopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
0.45 | - |
4-nitrophenyl beta-D-glucopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
0.45 | - |
4-nitrophenyl beta-D-fucopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
0.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
1.29 | - |
laminaribiose | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
1.4 | - |
laminaribiose | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
1.5 | - |
2-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
1.99 | - |
2-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
2.33 | - |
4-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
2.51 | - |
4-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
21.54 | - |
cellobiose | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
22.4 | - |
cellobiose | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
-20°C, in 50 mM sodium phosphate buffer, pH 7.0, 50% glycerol, no appreciable loss of activity even after several months | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + D-glucopyranose | - |
? | |
cellobiose + H2O | - |
Saccharolobus solfataricus | 2 beta-D-glucose | - |
? | |
cellotetraose + H2O | - |
Saccharolobus solfataricus | cellotriose + D-glucose | - |
? | |
laminaribiose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
additional information | broad specificity and remarkable glucan-1,4-beta-D-glucosidase (exocellobiase) activity | Saccharolobus solfataricus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Sbeta-gly | - |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | activity at 70°C is about 45% compared to the activity at 85°C. The temperatureactivity profiles of native and recombinant enzyme are almost indistinguishable using 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 100 | pH 6.5, no denaturation occurrs in the temperature range of 30 to 100°C for both the native and recombinant enzyme | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
427 | - |
4-nitrophenyl beta-D-fucopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
460 | - |
4-nitrophenyl beta-D-fucopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
524 | - |
laminaribiose | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
547 | - |
cellobiose | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
573 | - |
laminaribiose | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
634 | - |
4-nitrophenyl beta-D-glucopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
654 | - |
cellobiose | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
726 | - |
4-nitrophenyl beta-D-glucopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
930 | - |
4-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
1252 | - |
2-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
1341 | - |
4-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
1416 | - |
2-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
no denaturation occurrs in the temperature range of 30 to 100°C for both the native and recombinant enzyme | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
cellobiose | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
29 | - |
cellobiose | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
370 | - |
4-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
407 | - |
laminaribiose | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
410 | - |
laminaribiose | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
576 | - |
4-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
712 | - |
2-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
844 | - |
2-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
941 | - |
4-nitrophenyl beta-D-fucopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
1058 | - |
4-nitrophenyl beta-D-glucopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
1151 | - |
4-nitrophenyl beta-D-fucopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
1633 | - |
4-nitrophenyl beta-D-glucopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus |