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3.2.1.B26: Sulfolobus solfataricus beta-glycosidase

This is an abbreviated version!
For detailed information about Sulfolobus solfataricus beta-glycosidase, go to the full flat file.

Word Map on EC 3.2.1.B26

Reaction

Wide substrate specificity, active on aryl-beta-D-galactose, -alpha-L-fucose, -beta-D-glucose and -beta-D-xylose and on di- and oligosaccharides. D-Glucose dimers are hydrolysed in the order of decreasing efficiency: beta-(1,3), beta-(1,4), beta-(1,6). Exo-acting enzyme with a preference for cellotetraose. =

Synonyms

beta-D-glycosidase, beta-glucosidase, beta-Gly, beta-glycosidase, Bgl, bgly, EcSbgly, GH1 beta-glycosidase, LACS, Sbeta-gly, Sbetagly, Ss-beta-Gly, Ssbeta-Glc1, Ssbeta-Gly, SsbetaG, SsbetaGlc1, SsbetaGly, SsGH1, sso1353, SSO3019

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.B26 Sulfolobus solfataricus beta-glycosidase

Specific Activity

Specific Activity on EC 3.2.1.B26 - Sulfolobus solfataricus beta-glycosidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0019
wild type enzyme, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95°C
0.0072
mutant enzyme W361F, with ginsenoside C-Mc as substrate, at pH 5.5 and 95°C
0.012
mutant enzyme W361F, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95°C
0.0126
wild type enzyme, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95°C
0.0154
wild type enzyme, with ginsenoside C-Mc as substrate, at pH 5.5 and 95°C
0.0201
wild type enzyme, with ginsenoside Rc as substrate, at pH 5.5 and 95°C
0.0233
mutant enzyme W361F, with ginsenoside Rc as substrate, at pH 5.5 and 95°C
0.0355
mutant enzyme W361F, with naringin as substrate, at pH 5.5 and 95°C
0.0391
wild type enzyme, with hesperidin as substrate, at pH 5.5 and 95°C
0.0567
mutant enzyme L213A, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95°C
0.0661
wild type enzyme, with ginsenoside C-Mc as substrate, at pH 5.5 and 95°C
0.0802
mutant enzyme L213A, with ginsenoside Rd as substrate, at pH 5.5 and 95°C
0.146
mutant enzyme L213A, with ginsenoside C-Mc as substrate, at pH 5.5 and 95°C
0.211
wild type enzyme, with ginsenoside Rd as substrate, at pH 5.5 and 95°C
0.233
wild type enzyme, with naringin as substrate, at pH 5.5 and 95°C
0.288
mutant enzyme W361F, with hesperidin as substrate, at pH 5.5 and 95°C
0.338
wild type enzyme, with ginsenoside Rd as substrate, at pH 5.5 and 95°C
0.52
wild type enzyme, with ginsenoside Rb2 as substrate, at pH 5.5 and 95°C
0.54
mutant enzyme L213A, with ginsenoside Rb2 as substrate, at pH 5.5 and 95°C
0.58
pH 6.5, 75°C, enzyme immobilized on XAD-4
0.59
pH 6.5, 75°C, enzyme immobilized on XAD-16
1.18
mutant enzyme L213A, with ginsenoside C-Y as substrate, at pH 5.5 and 95°C
1.2
-
mutant E387G, substrate cellobiose, pH 3.0, 65°C
1.347
mutant enzyme W361F, with ginsenoside C-Y as substrate, at pH 5.5 and 95°C
1.415
mutant enzyme W361F, with ginsenoside Rd as substrate, at pH 5.5 and 95°C
1.643
wild type enzyme, with ginsenoside Rb2 as substrate, at pH 5.5 and 95°C
1.71
pH 6.5, 75°C, lyophilized enzyme
1.74
wild type enzyme, with ginsenoside C-Y as substrate, at pH 5.5 and 95°C
127
-
mutant E387G, substrate 2,4-dinitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C
13.13
wild type enzyme, with ginsenoside F2 as substrate, at pH 5.5 and 95°C
13.227
mutant enzyme W361F, with ginsenoside F2 as substrate, at pH 5.5 and 95°C
13.65
mutant enzyme L213A, with ginsenoside Rb1 as substrate, at pH 5.5 and 95°C
15
-
mutant E387G, substrate 4-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C
150
-
wild-type, substrate 4-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C
19.1
-
wild-type, substrate 2-nitrophenyl beta-D-xylopyranoside, pH 3.0, 65°C
196
-
wild-type, substrate 2,4-dinitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C
2.031
mutant enzyme W361F, with ginsenoside C-O as substrate, at pH 5.5 and 95°C
2.22
pH 6.5, 75°C, addition of 1% (w/v) SDS
2.5
-
mutant E387G, substrate 2-nitrophenyl beta-D-xylopyranoside, pH 3.0, 65°C
2.7
-
mutant E387G, substrate 2-nitrophenyl beta-D-galactopyranoside, pH 3.0, 65°C
219
-
wild-type, substrate 2-nitrophenyl beta-D-galactopyranoside, pH 3.0, 65°C
24.1
wild type enzyme, 1ith ginsenoside F2 as substrate, at pH 5.5 and 95°C
253
-
wild-type, substrate 2-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C
3.213
mutant enzyme W361F, with ginsenoside Rb1 as substrate, at pH 5.5 and 95°C
3.53
pH 6.5, 75°C, enzyme immobilized on Celite
33.4
mutant enzyme L213A, 1ith ginsenoside F2 as substrate, at pH 5.5 and 95°C
4.05
mutant enzyme L213A, with ginsenoside C-O as substrate, at pH 5.5 and 95°C
4.24
wild type enzyme, with ginsenoside C-O as substrate, at pH 5.5 and 95°C
4.388
wild type enzyme, with ginsenoside C-Y as substrate, at pH 5.5 and 95°C
5.082
wild type enzyme, with ginsenoside Rb1 as substrate, at pH 5.5 and 95°C
6.354
wild type enzyme, with ginsenoside C-O as substrate, at pH 5.5 and 95°C
61.9
-
wild-type, substrate cellobiose, pH 3.0, 65°C
63.3
-
mutant E387G, substrate 2-nitrophenyl beta-D-glucopyranoside, pH 3.0, 65°C
8.56
wild type enzyme, with ginsenoside Rb1 as substrate, at pH 5.5 and 95°C
9.077
mutant enzyme W361F, with ginsenoside Rb2 as substrate, at pH 5.5 and 95°C