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3.2.1.B26: Sulfolobus solfataricus beta-glycosidase

This is an abbreviated version!
For detailed information about Sulfolobus solfataricus beta-glycosidase, go to the full flat file.

Word Map on EC 3.2.1.B26

Reaction

Wide substrate specificity, active on aryl-beta-D-galactose, -alpha-L-fucose, -beta-D-glucose and -beta-D-xylose and on di- and oligosaccharides. D-Glucose dimers are hydrolysed in the order of decreasing efficiency: beta-(1,3), beta-(1,4), beta-(1,6). Exo-acting enzyme with a preference for cellotetraose. =

Synonyms

beta-D-glycosidase, beta-glucosidase, beta-Gly, beta-glycosidase, Bgl, bgly, EcSbgly, GH1 beta-glycosidase, LACS, Sbeta-gly, Sbetagly, Ss-beta-Gly, Ssbeta-Glc1, Ssbeta-Gly, SsbetaG, SsbetaGlc1, SsbetaGly, SsGH1, sso1353, SSO3019

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.B26 Sulfolobus solfataricus beta-glycosidase

Cloned

Cloned on EC 3.2.1.B26 - Sulfolobus solfataricus beta-glycosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned and expressed in Saccharomyces cerevisiae
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expressed in Escherichia coli
expressed in Escherichia coli Rosetta BL21(DE3)pLyS cells
expressed in Escherichia coli strain ER2566
expressed in Saccharomyces cerevisiae
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expression in Escherichia coli
expression in Escherichia coli BL21
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expression in Saccharomyces cerevisiae
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mutant S101C is expressed in Escherichia coli
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overexpressed in Escherichia coli
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wild-type and mutant enzymes are ontained by expression as fusions of glutathione S-transferase in Escherichia coli
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wild-type and mutated sequences are expressed in Escherichia coli with a His7-tag to allow one-step chromatographic purification
wild-type enzyme and mutant enzymes E432C and W433C are expressed in Escherichia coli with a His7-tag to allow one-step chromatographic purification
wild-type, mutant E432C, mutant W433C and mutant M439C enzymes are expressed in Escherichia coli as recombinant proteins containing an N-terminal His6-tag
-