3.2.1.B26: Sulfolobus solfataricus beta-glycosidase

This is an abbreviated version!
For detailed information about Sulfolobus solfataricus beta-glycosidase, go to the full flat file.

Reaction

Wide substrate specificity, active on aryl-beta-D-galactose, -alpha-L-fucose, -beta-D-glucose and -beta-D-xylose and on di- and oligosaccharides. D-Glucose dimers are hydrolysed in the order of decreasing efficiency: beta-(1,3), beta-(1,4), beta-(1,6). Exo-acting enzyme with a preference for cellotetraose. =

Synonyms

beta-D-glycosidase, beta-glucosidase, beta-Gly, beta-glycosidase, Bgl, bgly, EcSbgly, GH1 beta-glycosidase, LACS, Sbeta-gly, Sbetagly, Ss-beta-Gly, Ssbeta-Glc1, Ssbeta-Gly, SsbetaG, SsbetaGlc1, SsbetaGly, SsGH1, sso1353, SSO3019

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.B26 Sulfolobus solfataricus beta-glycosidase

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Results	in table
14	Activating Compound
1	AA Sequence
20	Application
29	Cloned(Commentary)
3	Crystallization (Commentary)
4	Expression
10	General Stability
37	Inhibitors
149	kcat/KM [mM/s]
13	Ki Value [mM]
197	KM Value [mM]
17	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
238	Organism
28	pH Optimum
7	pH Range
3	pH Stability
1	pI Value
77	Protein Variants
25	Purification (Commentary)
1	Reaction
74	Reference
1	Renatured (Commentary)
1	Source Tissue
57	Specific Activity [micromol/min/mg]
2	Storage Stability
216	Substrates and Products (Substrate)
16	Subunits
66	Synonyms
26	Temperature Optimum [°C]
7	Temperature Range [°C]
38	Temperature Stability [°C]
171	Turnover Number [1/s]

Temperature Range

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Temperature Range on EC 3.2.1.B26 - Sulfolobus solfataricus beta-glycosidase

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TEMPERATURE RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

30 - 75

Saccharolobus solfataricus

-

the specific activity of the mutant increases with temperature up to 75°C

724259

30 - 80

Saccharolobus solfataricus

-

enzyme immobilized on chitosan activated with glutaraldehyde shows an increase in the temperature range 30°C-80°C in 50 mM sodium phosphate buffer, pH 7.0

723982

30 - 95

Saccharolobus solfataricus

-

barely active at 30°C, optimal activity over 95°C

725304

70 - 80

Saccharolobus solfataricus

-

activity at 70°C is about 45% compared to the activity at 85°C. The temperatureactivity profiles of native and recombinant enzyme are almost indistinguishable using 2-nitrophenyl-beta-D-galactopyranoside

722128

80 - 100

Saccharolobus solfataricus

-

80°C: 65% of maximal activity, 100°C: 60% of maximal activity

724582

80 - 120

Saccharolobus solfataricus

-

80°C: about 40% of maximal activity, 110°C: 85% of maximal activity, 120°C: about 25% of maximal activity

724406

95 - 145

Saccharolobus solfataricus

-

95°C: about 45% of maximal activity, 145°C: about 50% of maximal activity

719097