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Literature summary for 3.2.1.B26 extracted from
- Hydrolysis of oleuropein by recombinant beta-glycosidase from hyperthermophilic archaeon Sulfolobus solfataricus immobilised on chitosan matrix (2000), J. Biotechnol., 77, 275-286.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | high yield production of hydroxytyrosol from a commercially available oleuropein by using the immobilised recombinant EcSbgly from the hyperthermophilic archaeon Sulfolobus solfataricus on chitosan support | Saccharolobus solfataricus |
General Stability
| General Stability | Organism |
|---|---|
| 18.2% of the enzyme is inactivated in the immobilisation process on lyophilised chitosan matrix | Saccharolobus solfataricus |
| the stability of the bioreactor at the operative temperatures shows a t1/2 of 30 days at 70°C and a t1/2 of 56 days at 60°C | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.1 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7.0, 75°C, soluble enzyme | Saccharolobus solfataricus |  |
| 5.3 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7.0, 75°C, immobilized enzyme | Saccharolobus solfataricus | |
| 21.6 | - |
cellobiose | pH and temperature not specified in the publication, immobilized enzyme | Saccharolobus solfataricus | |
| 32.7 | - |
cellobiose | pH and temperature not specified in the publication, soluble enzyme | Saccharolobus solfataricus | |
| 169 | - |
oleuropein | pH 7.0, 60°C, soluble enzyme | Saccharolobus solfataricus | |
| 240.4 | - |
oleuropein | pH 7.0, 70°C, soluble enzyme | Saccharolobus solfataricus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| cellobiose + H2O | - |
Saccharolobus solfataricus | beta-D-glucose + beta-D-glucose | - |
? | |
| oleuropein + H2O | i.e. (4S,5E,6S)-4-[2-[2-(3,4-dihydroxyphenyl)ethoxy]-2-oxoethyl]-5-ethylidene-6-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)-2-tetrahydropyranyl]oxy]-4H-pyran-3-carboxylic acid methyl ester. The biotransformation produces unstable aglycone species formed by oleuropein hydrolysis that gives rise to the formation of hydroxytyrosol, at the operative temperatures of the bioreactor. The results of the biotransformation at 70°C showed that the main products are hydroxytyrosol, and glucose, being the oleuropein aglycone present in low amount at the end of reaction | Saccharolobus solfataricus | oleuropein aglycone + D-glucopyranose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EcSbgly | - |
Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 274 | - |
oleuropein | pH 7.0, 60°C, soluble enzyme | Saccharolobus solfataricus | |
| 350.8 | - |
oleuropein | pH 7.0, 70°C, soluble enzyme | Saccharolobus solfataricus | |
| 628 | - |
cellobiose | pH and temperature not specified in the publication, soluble enzyme | Saccharolobus solfataricus | |
| 727 | - |
cellobiose | pH and temperature not specified in the publication, immobilized enzyme | Saccharolobus solfataricus | |
| 1662 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7.0, 75°C, immobilized enzyme | Saccharolobus solfataricus | |
| 1689 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7.0, 75°C, soluble enzyme | Saccharolobus solfataricus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.46 | - |
oleuropein | pH 7.0, 70°C, soluble enzyme | Saccharolobus solfataricus | |
| 1.62 | - |
oleuropein | pH 7.0, 60°C, soluble enzyme | Saccharolobus solfataricus | |
| 19.2 | - |
cellobiose | pH and temperature not specified in the publication, soluble enzyme | Saccharolobus solfataricus | |
| 33.7 | - |
cellobiose | pH and temperature not specified in the publication, immobilized enzyme | Saccharolobus solfataricus | |
| 313.6 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7.0, 75°C, immobilized enzyme | Saccharolobus solfataricus | |
| 412 | - |
2-nitrophenyl beta-D-galactopyranoside | pH 7.0, 75°C, soluble enzyme | Saccharolobus solfataricus | |
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