KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
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additional information | - |
additional information | kinetic constants for transglycosylation reactions | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
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Saccharolobus solfataricus | - |
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Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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additional information | mutant enzyme E387G catalyzes transglycosylation reactions. In the transglycosylation reactions, 2-nitrophenyl beta-D-glucopyranoside is used as the donor and different aryl and alkyl mono- and disaccharide substrates containing alpha- and beta-glycosidic linkages are used as acceptors. The formation of the beta-1,3 linkage is observed with acceptors containing the 2-nitrophenyl group, while beta-1,6-disaccharides are predominant with 4-nitrophenyl beta-D-glucoside. When a beta linkage of the acceptor is between two sugars, as for the formation of trisaccharides, the beta-1,3 acceptors possess affinity for the donor active site and are also partially hydrolyzed. The beta-1,4-based acceptor furnish only linear trisaccharides, albeit in very poor yield. The presence of alpha linkages in the acceptors induces beta-1,6 glycosylation despite the nature ofthe aglycons. In these cases, the yield is higher with the increasing lipophilic nature of the aglycon; however, the yield depends on the dimensions and nature of this group. In fact, while maltose as free disaccharide givse very poor yields of trisaccharide products, 33% yield is reached with 4-nitrophenyl alpha-D-glucopyranoside | Saccharolobus solfataricus | ? | - |
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Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
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additional information | - |
additional information | kinetic constants for transglycosylation reactions | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
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additional information | - |
additional information | kinetic constants for transglycosylation reactions | Saccharolobus solfataricus |