Literature summary for 3.2.1.B26 extracted from

Hamon, V.; Dallet, S.; Legoy, M.D.
The pressure-dependence of two beta-glucosidases with respect to their thermostability (1996), Biochim. Biophys. Acta, 1294, 195-203.

Search for more literature for 3.2.1.B26

Activating Compound

Activating Compound	Comment	Organism	Structure
4-nitrophenyl-beta-D-galactopyranoside	at low and intermediate temperatures (from 25°C to 50°C), the enzyme displays an inhibition by excess substrate and at high temperature (70 and 80°C) an activation, for 4-nitrophenyl-beta-D-galactopyranoside as substrate	Saccharolobus solfataricus
additional information	this enzyme appears to be activated by pressure between atmospheric pressure and 2.5 kbar with a maximal activity at 1.25 kbar. However, this enzyme still displayed the best catalytic efficiency at atmospheric pressure because of a Km value drastically increased by pressure	Saccharolobus solfataricus

General Stability

General Stability	Organism
highly barostable enzyme	Saccharolobus solfataricus

Inhibitors

Inhibitors	Comment	Organism	Structure
4-nitrophenyl-beta-D-galactopyranoside	at low and intermediate temperatures (from 25°C to 50°C), the enzyme displays an inhibition by excess substrate and at high temperature (70 and 80°C) an activation, for 4-nitrophenyl-beta-D-galactopyranoside as substrate	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.085	-	4-nitrophenyl-beta-D-glucopyranoside	pH 6.0, 60°C, 1 bar	Saccharolobus solfataricus
0.16	-	4-nitrophenyl-beta-D-glucopyranoside	pH 6.0, 60°C, 500 bar	Saccharolobus solfataricus
0.41	-	4-nitrophenyl-beta-D-glucopyranoside	pH 6.0, 60°C, 750 bar	Saccharolobus solfataricus
0.55	-	4-nitrophenyl-beta-D-glucopyranoside	pH 6.0, 60°C, 1000 bar	Saccharolobus solfataricus
0.86	-	4-nitrophenyl-beta-D-glucopyranoside	pH 6.0, 60°C, 1500 bar	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-galactopyranoside + H2O	-	Saccharolobus solfataricus	4-nitrophenol + beta-D-galactopyranose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Saccharolobus solfataricus	4-nitrophenol + beta-D-glucopyranose	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
100	-	-	Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
80	120	80°C: about 40% of maximal activity, 110°C: 85% of maximal activity, 120°C: about 25% of maximal activity	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
5	-	after a storage greater than 40 h at atmospheric pressure, the residual activity decreases	Saccharolobus solfataricus
60	-	maintains a half-life of 91 h at 60°C and 2.5 kbar	Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1764	-	4-nitrophenyl-beta-D-glucopyranoside	pH 6.0, 60°C, 1500 bar	Saccharolobus solfataricus
3356	-	4-nitrophenyl-beta-D-glucopyranoside	pH 6.0, 60°C, 1000 bar	Saccharolobus solfataricus
5811	-	4-nitrophenyl-beta-D-glucopyranoside	pH 6.0, 60°C, 750 bar	Saccharolobus solfataricus
11130	-	4-nitrophenyl-beta-D-glucopyranoside	pH 6.0, 60°C, 500 bar	Saccharolobus solfataricus
11910	-	4-nitrophenyl-beta-D-glucopyranoside	pH 6.0, 60°C, 1 bar	Saccharolobus solfataricus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)