3.2.1.B26: Sulfolobus solfataricus beta-glycosidase

This is an abbreviated version!
For detailed information about Sulfolobus solfataricus beta-glycosidase, go to the full flat file.

Reaction

Wide substrate specificity, active on aryl-beta-D-galactose, -alpha-L-fucose, -beta-D-glucose and -beta-D-xylose and on di- and oligosaccharides. D-Glucose dimers are hydrolysed in the order of decreasing efficiency: beta-(1,3), beta-(1,4), beta-(1,6). Exo-acting enzyme with a preference for cellotetraose. =

Synonyms

beta-D-glycosidase, beta-glucosidase, beta-Gly, beta-glycosidase, Bgl, bgly, EcSbgly, GH1 beta-glycosidase, LACS, Sbeta-gly, Sbetagly, Ss-beta-Gly, Ssbeta-Glc1, Ssbeta-Gly, SsbetaG, SsbetaGlc1, SsbetaGly, SsGH1, sso1353, SSO3019

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.B26 Sulfolobus solfataricus beta-glycosidase

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Results	in table
14	Activating Compound
1	AA Sequence
20	Application
29	Cloned(Commentary)
3	Crystallization (Commentary)
4	Expression
10	General Stability
37	Inhibitors
149	kcat/KM [mM/s]
13	Ki Value [mM]
197	KM Value [mM]
17	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
238	Organism
28	pH Optimum
7	pH Range
3	pH Stability
1	pI Value
77	Protein Variants
25	Purification (Commentary)
1	Reaction
74	Reference
1	Renatured (Commentary)
1	Source Tissue
57	Specific Activity [micromol/min/mg]
2	Storage Stability
216	Substrates and Products (Substrate)
16	Subunits
66	Synonyms
26	Temperature Optimum [°C]
7	Temperature Range [°C]
38	Temperature Stability [°C]
171	Turnover Number [1/s]

General Stability

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General Stability on EC 3.2.1.B26 - Sulfolobus solfataricus beta-glycosidase

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GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

18.2% of the enzyme is inactivated in the immobilisation process on lyophilised chitosan matrix

Saccharolobus solfataricus

-

725589

chaotropes (I-, ClO4-, NO3- and Cs+) have a strong destabilising effect on the protein by perturbing hydrophobic interactions

Saccharolobus solfataricus

-

722214

denaturing effect of NaCl and Na2SO4

Saccharolobus solfataricus

-

722214

half-life times of the enzyme in hydrolysis of lactose is carried out at 70°C in a continuous stirred-tank reactor coupled to a 10000 Da cross-flow ultrafiltration module (to recycle the enzyme) is approximately 5 to 7 days

Saccharolobus solfataricus

-

724625

highly barostable enzyme

Saccharolobus solfataricus

-

724406

in Escherichia coli

Saccharolobus solfataricus

-

725356

N-epsilon-methylated beta-glycosidase from Sulfolobus solfataricus is characterized by a higher resistance to aggregation and denaturation at physiological pH, in comparison with the unmethylated form recombinantly expressed

Saccharolobus solfataricus

-

725356

stable in the presence of detergents

Saccharolobus solfataricus

-

724477

the enzyme can be immobilized with little loss of enzyme activity and catalytic efficiency by covalent coupling of the proteins via the surface amino groups to insoluble carriers

Saccharolobus solfataricus

-

724626

the stability of the bioreactor at the operative temperatures shows a t1/2 of 30 days at 70°C and a t1/2 of 56 days at 60°C

Saccharolobus solfataricus

-

725589