Literature summary for 3.2.1.B26 extracted from

Bismuto, E.; Nucci, R.; Febbraio, F.; Tanfani, F.; Gentile, F.; Briante, R.; Scire, A.; Bertoli, E.; Amodeo, P.
Effects induced by mono- and divalent cations on protein regions responsible for thermal adaptation in beta-glycosidase from Sulfolobus solfataricus (2004), Eur. Biophys. J., 33, 38-49.

Search for more literature for 3.2.1.B26

Inhibitors

Inhibitors	Comment	Organism	Structure
Ba2+	the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+	Saccharolobus solfataricus
Ca2+	the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+	Saccharolobus solfataricus
K+	the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+	Saccharolobus solfataricus
Li+	the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+	Saccharolobus solfataricus
Mg2+	the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+	Saccharolobus solfataricus
additional information	cations can cause a strong attenuation of the ion pair interactions E474K72 and D473R402, with consequent partial dissociation of the tetrameric structure	Saccharolobus solfataricus
Na+	the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.84	-	4-nitrophenyl beta-D-galactopyranoside	pH 7.5, 75°C	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-galactopyranoside + H2O	-	Saccharolobus solfataricus	4-nitrophenol + beta-D-galactopyranose	-	?

Subunits

Subunits	Comment	Organism
tetramer	-	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
Sbetagly	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	assay at	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	the thermostability is affected by the nature and charge of the cations, reaching maximal effects for the case of Mg2+. Cations can cause a strong attenuation of the ion pair interactions E474K72 and D473R402, with consequent partial dissociation of the tetrameric structure	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
445.6	-	4-nitrophenyl beta-D-galactopyranoside	pH 7.5, 75°C	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	K+	above 200 mM, pH 7.5, 75°C	Saccharolobus solfataricus
12	13	Mg2+	pH 7.5, 75°C	Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
530.4	-	4-nitrophenyl beta-D-galactopyranoside	pH 7.5, 75°C	Saccharolobus solfataricus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)