3.2.1.B26: Sulfolobus solfataricus beta-glycosidase
This is an abbreviated version!
For detailed information about Sulfolobus solfataricus beta-glycosidase, go to the full flat file.
Word Map on EC 3.2.1.B26
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3.2.1.B26
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transglycosylation
-
synthesis
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beta-d-galactoside
-
beta-glucosides
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amygdalin
-
glycone
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3.2.1.21
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transgalactosylation
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prunasin
-
beta-d-fucoside
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food industry
-
analysis
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nutrition
- 3.2.1.B26
-
transglycosylation
- synthesis
- beta-d-galactoside
- beta-glucosides
- amygdalin
-
glycone
-
3.2.1.21
-
transgalactosylation
- prunasin
- beta-d-fucoside
- food industry
- analysis
- nutrition
Reaction
Wide substrate specificity, active on aryl-beta-D-galactose, -alpha-L-fucose, -beta-D-glucose and -beta-D-xylose and on di- and oligosaccharides. D-Glucose dimers are hydrolysed in the order of decreasing efficiency: beta-(1,3), beta-(1,4), beta-(1,6). Exo-acting enzyme with a preference for cellotetraose. =
Synonyms
beta-D-glycosidase, beta-glucosidase, beta-Gly, beta-glycosidase, Bgl, bgly, EcSbgly, GH1 beta-glycosidase, LACS, Sbeta-gly, Sbetagly, Ss-beta-Gly, Ssbeta-Glc1, Ssbeta-Gly, SsbetaG, SsbetaGlc1, SsbetaGly, SsGH1, sso1353, SSO3019
ECTree
3.2.1.B26 Sulfolobus solfataricus beta-glycosidaseAdvanced search results
results (
results (KM Value
KM Value on EC 3.2.1.B26 - Sulfolobus solfataricus beta-glycosidase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.17
2,4-dinitrophenyl beta-D-glucopyranoside
-
pH 6.5, 65°C, wild-type enzyme
1.3
4-methylumbelliferyl beta-D-glucuronide
pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme
24.2
4-nitrophenyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme
192
methyl beta-D-galactoside
release of methanol, pH 7.5, 80°C
0.95
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65°C, wild-type enzyme
0.95
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65°C, mutant enzyme R488A
0.99
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65°C, wild-type enzyme
1.47
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65°C, mutant enzyme R489A
1.5
2-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
1.53
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65°C, mutant enzyme E206Q
1.9
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65°C, mutant enzyme His489
1.97
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65°C, mutant enzyme delVal484-His489
1.99
2-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, native enzyme
3.9
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 75°C, recombinant enzyme
3.9
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75°C, soluble enzyme
4
2-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 75°C, wild-type enzyme
4.1
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75°C, soluble enzyme
5
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75°C, enzyme immobilized on chitosan activated with glutaraldehyde
5.3
2-nitrophenyl beta-D-galactopyranoside
-
pH 7.0, 75°C, immobilized enzyme
1.1
2-nitrophenyl beta-D-galactoside
release of 2-nitrophenol, pH 7.5, 80°C
0.5
2-nitrophenyl beta-D-glucopyranoside
-
wild-type, pH 6.5, 65°C, presence of 2 M formate
1.01
2-nitrophenyl beta-D-glucopyranoside
-
pH 6.5, 65°C, wild-type enzyme
1.01
2-nitrophenyl beta-D-glucopyranoside
-
wild-type, pH 6.5, 65°C
1.1
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 6.0, 65°C
1.17
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 6.5, 65°C, presence of 2 M formate
2.2
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 5.0, 65°C
4.9
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 4.0, 65°C
16.4
2-nitrophenyl beta-D-glucopyranoside
-
mutant E387G, pH 3.0, 65°C
0.03
2-Nitrophenyl beta-D-glucoside
-
pH 6.5, 65°C, mutant enzyme E206Q
1.01
2-Nitrophenyl beta-D-glucoside
-
pH 6.5, 65°C, wild-type enzyme
0.011
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80°C, wild-type enzyme
0.023
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80°C, mutant enzyme M439C
0.34
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80°C, mutant enzyme E432C
0.41
4-methylumbelliferyl beta-D-fucopyranoside
-
pH 6.5, 80°C, mutant enzyme W433C
0.011
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme
0.011
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80°C, wild-type enzyme
0.21
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 45°C, wild-type enzyme
0.34
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C
0.34
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80°C, mutant enzyme E432C
0.41
4-methylumbelliferyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C
0.41
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 80°C, mutant enzyme W433C
18.6
4-methylumbelliferyl beta-D-fucoside
pH 6.5, 45°C, mutant enzyme W433C
0.066
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80°C, wild-type enzyme
0.083
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80°C, mutant enzyme M439C
0.47
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80°C, mutant enzyme E432C
2.2
4-methylumbelliferyl beta-D-galactopyranoside
-
pH 6.5, 80°C, mutant enzyme W433C
0.066
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme
0.066
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80°C, wild-type enzyme
0.47
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C
0.47
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80°C, mutant enzyme E432C
0.57
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45°C, wild-type enzyme
0.81
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45°C, mutant enzyme E432C
2.2
4-methylumbelliferyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C
2.2
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 80°C, mutant enzyme W433C
5.28
4-methylumbelliferyl beta-D-galactoside
pH 6.5, 45°C, mutant enzyme W433C
1.3
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 80°C, wild-type enzyme
2.72
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 45°C, mutant enzyme W433C
3.46
4-methylumbelliferyl beta-D-galacturonate
pH 6.5, 45°C, mutant enzyme E432C
0.046
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80°C, wild-type enzyme
0.068
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80°C, mutant enzyme M439C
0.34
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80°C, mutant enzyme E432C
1.61
4-methylumbelliferyl beta-D-glucopyranoside
-
pH 6.5, 80°C, mutant enzyme W433C
0.046
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme
0.046
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80°C, wild-type enzyme
0.15
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45°C, wild-type enzyme
0.34
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C
0.34
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80°C, mutant enzyme E432C
0.51
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45°C, mutant enzyme E432C
1.61
4-methylumbelliferyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C
1.61
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 80°C, mutant enzyme W433C
22.1
4-methylumbelliferyl beta-D-glucoside
pH 6.5, 45°C, mutant enzyme W433C
1.3
4-methylumbelliferyl beta-D-glucuronic acid
-
pH 6.5, 80°C, wild-type enzyme
1.4
4-methylumbelliferyl beta-D-glucuronic acid
-
pH 6.5, 80°C, mutant enzyme M439C
0.036
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80°C, wild-type enzyme
0.042
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80°C, mutant enzyme M439C
0.18
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80°C, mutant enzyme W433C
0.9
4-methylumbelliferyl beta-D-mannopyranoside
-
pH 6.5, 80°C, mutant enzyme E432C
0.036
4-methylumbelliferyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme
0.036
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80°C, wild-type enzyme
0.18
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80°C, mutant enzyme W433C
0.9
4-methylumbelliferyl beta-D-mannoside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C
0.9
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 80°C, mutant enzyme E432C
24.2
4-methylumbelliferyl beta-D-mannoside
pH 6.5, 45°C, wild-type enzyme
0.068
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80°C, mutant enzyme M439C
0.13
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80°C, wild-type enzyme
0.59
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80°C, mutant enzyme W433C
1.26
4-methylumbelliferyl beta-D-xylopyranoside
-
pH 6.5, 80°C, mutant enzyme E432C
0.13
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80°C, wild-type enzyme
0.13
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80°C, wild-type enzyme
0.59
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme W433C
0.59
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80°C, mutant enzyme W433C
1.26
4-methylumbelliferyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 80°C, mutant enzyme E432C
1.26
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 80°C, mutant enzyme E432C
5.1
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 45°C, wild-type enzyme
13.7
4-methylumbelliferyl beta-D-xyloside
pH 6.5, 45°C, mutant enzyme E432C
0.4
4-nitrophenyl beta-D-fucopyranoside
-
pH and temperature not specified in the publication, native enzyme
0.45
4-nitrophenyl beta-D-fucopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
0.09
4-nitrophenyl beta-D-fucoside
-
pH 6.5, 65°C, mutant enzyme E206Q
0.21
4-nitrophenyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme
0.45
4-nitrophenyl beta-D-fucoside
-
pH 6.5, 65°C, wild-type enzyme
0.7
4-nitrophenyl beta-D-fucoside
-
pH 6.5, 75°C, recombinant enzyme
18.6
4-nitrophenyl beta-D-fucoside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C
1.17
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65°C, wild-type enzyme
2.33
4-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, native enzyme
2.51
4-nitrophenyl beta-D-galactopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
4.3
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 75°C, recombinant enzyme
4.7
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 75°C, wild-type enzyme
4.79
4-nitrophenyl beta-D-galactopyranoside
-
pH 6.5, 65°C, mutant enzyme E206Q
0.57
4-nitrophenyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme
0.81
4-nitrophenyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C
5.28
4-nitrophenyl beta-D-galactoside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C
0.45
4-nitrophenyl beta-D-glucopyranoside
-
pH and temperature not specified in the publication, native enzyme
0.6
4-nitrophenyl beta-D-glucopyranoside
-
pH and temperature not specified in the publication, recombinant enzyme
0.03
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 65°C, mutant enzyme E206Q
0.15
4-nitrophenyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme
0.3
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 65°C, wild-type enzyme
0.5
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 75°C, recombinant enzyme
0.5
4-nitrophenyl beta-D-glucoside
-
pH 6.5, 75°C, wild-type enzyme
0.51
4-nitrophenyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C
22.1
4-nitrophenyl beta-D-glucoside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme W433C
5.1
4-nitrophenyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme
13.7
4-nitrophenyl beta-D-xyloside
pH 6.5 (50 mM phosphate), 45°C, mutant enzyme E432C
0.55
4-nitrophenyl-beta-D-glucopyranoside
-
pH 6.0, 60°C, 1000 bar
0.86
4-nitrophenyl-beta-D-glucopyranoside
-
pH 6.0, 60°C, 1500 bar
20
cellobiose
-
pH 7.0, 75°C, enzyme immobilized on chitosan activated with glutaraldehyde
21.54
cellobiose
-
pH and temperature not specified in the publication, recombinant enzyme
21.6
cellobiose
-
pH and temperature not specified in the publication, immobilized enzyme
22.4
cellobiose
-
pH and temperature not specified in the publication, native enzyme
32.7
cellobiose
-
pH and temperature not specified in the publication, soluble enzyme
1.93
ginsenoside Rb1
mutant enzyme W361F, at pH 5.5 and 95°C
1.59
ginsenoside Rd
mutant enzyme W361F, at pH 5.5 and 95°C
131
lactose
-
pH 5.5, 70°C, enzyme immobilized on controlled pore glass
1.29
laminaribiose
-
pH and temperature not specified in the publication, native enzyme
1.4
laminaribiose
-
pH and temperature not specified in the publication, recombinant enzyme
additional information
additional information
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kinetic studies of the reactions of galactosylated enzyme intermediates with a range of nucleophiles
-
additional information
additional information
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KM increases with temperature: the increase is moderate at lower temperatures (from 30°C to 65°C) and more evident between 65°C and 85°C. Reactions with 4-nitrophenyl-beta-D-glucoside and 2-nitrophenyl-beta-D-glucoside show a biphasic behavior in LineweaverBurk plots, suggesting that transglycosylation reactions occur at higher substrate concentrations. Kinetic constants obtained at lower or higher substrate concentrations are calculated
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additional information
additional information
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kinetic constant values for soluble enzyme and for enzyme immobilized on chitosan activated with glutaraldehyde are comparable
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additional information
additional information
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kinetic constants for transglycosylation reactions
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