Literature summary for 3.2.1.B26 extracted from

Gloster, T.M.; Roberts, S.; Ducros, V.M.; Perugino, G.; Rossi, M.; Hoos, R.; Moracci, M.; Vasella, A.; Davies, G.J.
Structural studies of the beta-glycosidase from Sulfolobus solfataricus in complex with covalently and noncovalently bound inhibitors (2004), Biochemistry, 43, 6101-6109.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
structures, at approximately 2 A resolution, of the enzyme in complex with both covalently (derived from 2-fluoro-glycosides) and noncovalently (hydroximolactam) bound inhibitors	Saccharolobus solfataricus

Inhibitors

Inhibitors	Comment	Organism	Structure
D-galactohydroximolactam	-	Saccharolobus solfataricus
D-glucohydroximolactam	-	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.16	-	4-nitrophenyl beta-D-glucopyranoside	pH 6.5, 37°C	Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56690	-	quadrupole time-of-flight electrospray mass spectrometry	Saccharolobus solfataricus
56692	-	x * 56692, calculated from sequence	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	P22498	-	-
Saccharolobus solfataricus P2	P22498	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Saccharolobus solfataricus	4-nitrophenol + D-glucopyranose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Saccharolobus solfataricus P2	4-nitrophenol + D-glucopyranose	-	?
additional information	the enzyme has broad specificity, accommodating both gluco- and galacto-configured substrates	Saccharolobus solfataricus	?	-	?
additional information	the enzyme has broad specificity, accommodating both gluco- and galacto-configured substrates	Saccharolobus solfataricus P2	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 56692, calculated from sequence	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
GH1 beta-glycosidase	-	Saccharolobus solfataricus
Ssbeta-Glc1	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.2	-	4-nitrophenyl beta-D-glucopyranoside	pH 6.5, 37°C	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.001	-	D-glucohydroximolactam	pH 6.5, 37°C	Saccharolobus solfataricus
0.0011	-	D-galactohydroximolactam	pH 6.5, 37°C	Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
26.25	-	4-nitrophenyl beta-D-glucopyranoside	pH 6.5, 37°C	Saccharolobus solfataricus

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Release 2023.1 (January 2023)