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3.2.1.78: mannan endo-1,4-beta-mannosidase

This is an abbreviated version!
For detailed information about mannan endo-1,4-beta-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.78

Reaction

Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
+
H2O
=
Manbeta(1-4)Manbeta(1-4)Man
 +
Manbeta(1-4)Man

Synonyms

(1,4)-beta-D-mannan mannanohydrolase, 1,4-beta-D-mannan mannanohydrolase, 1,4-beta-mannanase, beta-1,4-D-mannanase, beta-1,4-mannan 4-mannanohydrolase, beta-1,4-mannanase, beta-D-mannanase, Beta-mannanase, beta-mannanase B, CaMan, CelB, cold-adapted beta-mannanase, CsMan5, CtManf, Dtur_0671, em26a, endo-1,4-beta-D-mannanase, endo-1,4-beta-mannanase, endo-1,4-mannanase, endo-acting beta-1,4-mannanase, endo-beta 1,4-mannanase, endo-beta-(1,4)-mannanase, endo-beta-(1->4)-mannanase, endo-beta-1,4 mannanase, endo-beta-1,4,D-mannanase, endo-beta-1,4-D-mannanase, endo-beta-1,4-mannanase, endo-beta-1,4-mannase, endo-beta-D-1,4-mannanase, endo-beta-D-mannanase, endo-beta-mannanase, endo-mannanase, GH 134 beta-1,4-mannanase, GH134, GH5-CBM27, KMAN-3, LeMAN4, LeMAN4a, Man, MAN I, Man II, MAN-P, Man1, Man113A, Man134A, Man26A, Man26A-50K, Man26b, Man4A, Man5, Man5A, Man5C, man5D, Man5P1, Man5XZ3, Man5_8, Man7, ManA, ManA/HmA, ManB, ManB-1601, ManC, ManEM17, ManF, ManH, ManIII, mannan endo-1,4-beta-mannanase, mannan endo-1,4-beta-mannosidase, mannan endo-1.4-beta-D-mannosidase, mannanase, mannanase, endo-1,4-beta-, ManP, ManS2, MYCTH_99077, PoMan5A, Rman, TpMan, TrMan5A

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.78 mannan endo-1,4-beta-mannosidase

Cloned

Cloned on EC 3.2.1.78 - mannan endo-1,4-beta-mannosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
both ManA and ManB
-
cloning in Escherichia coli strain JM109, functional overexpression in Pichia pastoris strain GS115, the enzyme is secreted
expressed in Arabidopsis thaliana transformed with Agrobacterium tumefaciens
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 CodonPlus(DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells and Bacillus subtilis WB600 cells
-
expressed in Escherichia coli JM109 cells
-
expressed in Escherichia coli strain BL21
expressed in Escherichia coli strain KMAN-3
-
expressed in Escherichia coli XL10-Gold cells
-
expressed in Pichia pastoris
expressed in Pichia pastoris nGS115 cells
expressed in Pichia pastoris strain GS115
expressed in Pichia pastoris X33 cells
expressed in Saccharomyces cerevisiae and Escherichia coli
-
expressed in Saccharomyces cerevisiae strain BY4741
-
expression in Aspergillus niger
expression in Aspergillus oryzae
-
expression in Aspergillus sojae and Pichia pastoris
expression in Escherichia coli
expression in in Aspergillus sojae and Pichia pastoris
expression in Pichia pastoris
expression in Pichia pastoris and Escherichia coli
expression in Yarrowia lipolytica
-
expression of wild-type and mutant enzyme in Pichia pastoris
exprression in Escherichia coli fused with thioredoxin gene
gene An15g07760, cloning in Escherichia coli, and expression of recombinant chimeric xylanase/mannanase fusion enzymes in Pichia pastoris strain X33 using GAP promoter, secretion to the medium
gene HhMan, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of C-terminally His6-tagged extracellular enzyme in and secretion from Pichia pastoris strain GS115
-
gene man5, DNA and amino acid sequence determination and analysis
gene man5A, DNA and amino acid sequence determination and analysis, recombinant expression in Pichia pastoris strain GS115
-
gene man5F63, DNA and amino acid sequence determination and analysis, sequence comparisons, cloning in Escherichia coli strain Trans1-T1, overexpression in Pichia pastoris strain GS115 and secretion to the culture medium
gene man5P1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of extracellular enzyme in Pichia pastoris strain GS115
-
gene man5XZ3, sequence comparisons, cloning in Escherichia coli strain Trans1-T1, and recombinant expression of wild-type and mutant enzymes fused in frame to the DNA sequence for the Saccharomyces cerevisiae alpha-factor secretory signal peptide and under the control of the methanol-inducible alcohol oxidase promoter in Pichia pastoris strain GS115, induction by methanol, the proteins are secreted
-
gene manA, recombinant expression in and secretion from Yarrowia lipolytica
-
gene poman5A, recombinant expression of C-terminally His6-tagged extracellular enzyme in and secretion from Pichia pastoris strain GS115
-
gene RmMan5A, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged enzyme in Escherichia coli strain BL21
-
gene Tpet_1542, recombinant expression of His-tagged full-length enzyme and isolated catalytic domain in Escerichia coli strain BL21(DE3)DELTASlyD
recombinant expression of His-tagged full-length enzyme and catalytic domain
the full-length gene (Man1) and two truncated genes (Man1DELTACBM lacking CBM domain and Man1DELTALCBM lacking linker and CBM domains) from Trichoderma reesei Rut C30 are cloned and expressed in Pichia pastoris