Any feedback?
Literature summary for 3.2.1.78 extracted from
- A celluloytic complex from Clostridium cellulovorans consisting of mannanase B and endoglucanase E has synergistic effects on galactomannan degradation (2011), Appl. Microbiol. Biotechnol., 90, 565-572.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | an assembly of ManB with mini-CbpA, which contains a carbohydrate-binding module that provides proximity to insoluble substrates, increases the activity toward galactomannan from locust bean gum and guar gum 1.7- and 2.0fold over those without mini-CbpA | Clostridium cellulovorans |
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | when assembled with the mini-CbpA, which contains a carbohydrate-binding module that provides proximity to insoluble substrates, a mixture of endoglucanase E and ManB at a molar ratio of 1:2 shows the highest synergistic effect of 2.4fold on locust bean gum degradation. The mixture at a ratio of 1:3 shows the highest synergistic effect of 2.8fold on guar gum | Clostridium cellulovorans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Clostridium cellulovorans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 70000, SDS-PAGE, recombinant protein including His-tag | Clostridium cellulovorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium cellulovorans | - |
member of glycoyl hydrolase family 26 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium cellulovorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.2 | - |
substrate guar gum, pH 7.0, 40°C | Clostridium cellulovorans |
| 12.2 | - |
substrate locust bean gum, pH 7.0, 40°C | Clostridium cellulovorans |
| 18.5 | - |
substrate glucomannan, pH 7.0, 40°C | Clostridium cellulovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| galactoglucomannan + H2O | - |
Clostridium cellulovorans | ? | - |
? |  |
| guar gum + H2O | - |
Clostridium cellulovorans | ? | - |
? | |
| locust bean gum + H2O | - |
Clostridium cellulovorans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, SDS-PAGE, recombinant protein including His-tag | Clostridium cellulovorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ManB | - |
Clostridium cellulovorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Clostridium cellulovorans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | more than 80% of maximum activity | Clostridium cellulovorans |
| 50 | - |
rapid decrease in activity above | Clostridium cellulovorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Clostridium cellulovorans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
