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Literature summary for 3.2.1.78 extracted from
- Recombinant production and characterisation of two related GH5 endo-beta-1,4-mannanases from Aspergillus nidulans FGSC A4 showing distinctly different transglycosylation capacity (2011), Biochim. Biophys. Acta, 1814, 1720-1729.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Aspergillus nidulans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S289W | mutation lowers KM for mannooligosaccharides by 30-45% and increases transglycosylation yield by 50% compared to wild-type | Aspergillus nidulans |
| W283S | mutation in subsite +1, mutation results in increase in KM value, reduction in the transglycosylation yield by 30-45% and decrease in activity towards mannans | Aspergillus nidulans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | wild-type, pH 5.5, 37°C | Aspergillus nidulans |  |
| 1 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
| 1.3 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
| 1.6 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
| 1.8 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | pH 5.5, 37°C | Aspergillus nidulans | |
| 1.8 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | wild-type, pH 5.5, 37°C | Aspergillus nidulans | |
| 2.8 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
| 2.9 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | pH 5.5, 37°C | Aspergillus nidulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43847 | - |
x * 56000, SDS-PAGE, x * 43847, calculated | Aspergillus nidulans |
| 46644 | - |
x * 56000, SDS-PAGE, x * 46644, calculated | Aspergillus nidulans |
| 56000 | - |
x * 56000, SDS-PAGE, x * 43847, calculated | Aspergillus nidulans |
| 56000 | - |
x * 56000, SDS-PAGE, x * 46644, calculated | Aspergillus nidulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | Q5AZ53 | member of glycosyl hydrolase family 5 | - |
| Aspergillus nidulans | Q5B7X2 | member of glycosyl hydrolase family 5 | - |
| Aspergillus nidulans FGSC A4 | Q5AZ53 | member of glycosyl hydrolase family 5 | - |
| Aspergillus nidulans FGSC A4 | Q5B7X2 | member of glycosyl hydrolase family 5 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | treatment by endoglycosidase H decreases molecular mass to 46000 Da | Aspergillus nidulans |
| glycoprotein | treatment by endoglycosidase H decreases molecular mass to 51000 Da | Aspergillus nidulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guar gum glucomannan + H2O | - |
Aspergillus nidulans | ? | - |
? | |
| guar gum glucomannan + H2O | - |
Aspergillus nidulans FGSC A4 | ? | - |
? | |
| konjac glucomannan + H2O | - |
Aspergillus nidulans | ? | - |
? | |
| konjac glucomannan + H2O | - |
Aspergillus nidulans FGSC A4 | ? | - |
? | |
| locust bean gum glucomannan + H2O | - |
Aspergillus nidulans | ? | - |
? | |
| locust bean gum glucomannan + H2O | - |
Aspergillus nidulans FGSC A4 | ? | - |
? | |
| Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4 mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans | 2 Man-beta-1,4-Man | 64% of product | ? | |
| Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4-mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans | 2 Man-beta-1,4-Man | 68% of product | ? | |
| Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4-mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans FGSC A4 | 2 Man-beta-1,4-Man | 68% of product | ? | |
| Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4 mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans FGSC A4 | 2 Man-beta-1,4-Man | 64% of product | ? | |
| Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4 mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans | Man-beta-1,4-Man-beta-1,4-Man + Man-beta-1,4-Man | sole products | ? | |
| Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4-mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans | Man-beta-1,4-Man-beta-1,4-Man + Man-beta-1,4-Man | sole products | ? | |
| Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4 mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans | Man-beta-1,4-Man + Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | 88% of products | ? | |
| Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O | increasing catalytic efficiency towards beta-1,4-mannooligosaccharides with the degree of polymerisation from 4 to 6 | Aspergillus nidulans | Man-beta-1,4-Man + Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | 88% of products | ? | |
| additional information | enzyme displays good transgycosylation activity using mannotriose, melezitose and isomaltotriose as acceptors | Aspergillus nidulans | ? | - |
? | |
| additional information | enzyme displays transglycosylation activity using only mannotriose as acceptor | Aspergillus nidulans | ? | - |
? | |
| additional information | enzyme displays good transgycosylation activity using mannotriose, melezitose and isomaltotriose as acceptors | Aspergillus nidulans FGSC A4 | ? | - |
? | |
| additional information | enzyme displays transglycosylation activity using only mannotriose as acceptor | Aspergillus nidulans FGSC A4 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, SDS-PAGE, x * 43847, calculated | Aspergillus nidulans |
| ? | x * 56000, SDS-PAGE, x * 46644, calculated | Aspergillus nidulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ManA | - |
Aspergillus nidulans |
| ManC | - |
Aspergillus nidulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus nidulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
3 h, 95% residual activity | Aspergillus nidulans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
| 67 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | pH 5.5, 37°C | Aspergillus nidulans | |
| 98 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
| 112 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | wild-type, pH 5.5, 37°C | Aspergillus nidulans | |
| 117 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
| 134 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | wild-type, pH 5.5, 37°C | Aspergillus nidulans | |
| 148 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
| 193 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | pH 5.5, 37°C | Aspergillus nidulans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Aspergillus nidulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | more than 95% of maximum activity | Aspergillus nidulans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 23 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | pH 5.5, 37°C | Aspergillus nidulans | |
| 27 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
| 36 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
| 61 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | wild-type, pH 5.5, 37°C | Aspergillus nidulans | |
| 109 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | pH 5.5, 37°C | Aspergillus nidulans | |
| 115 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
| 118 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | mutant W283S, pH 5.5, 37°C | Aspergillus nidulans | |
| 215 | - |
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man | wild-type, pH 5.5, 37°C | Aspergillus nidulans | |
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