Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain BY4741 | Aspergillus sulphureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus sulphureus | - |
- |
- |
Aspergillus sulphureus CGMCC0608 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme has three N-glycosylation sites (N173, N242, N313) and two O-glycosylation sites (S5, S334) | Aspergillus sulphureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
konjac glucomannan + H2O | - |
Aspergillus sulphureus | mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose | - |
? | |
konjac glucomannan + H2O | - |
Aspergillus sulphureus CGMCC0608 | mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose | - |
? | |
locust bean gum galactomannan + H2O | - |
Aspergillus sulphureus | mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose | - |
? | |
locust bean gum galactomannan + H2O | - |
Aspergillus sulphureus CGMCC0608 | mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Beta-mannanase | - |
Aspergillus sulphureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aspergillus sulphureus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | more than 50% activity between 30 and 70°C | Aspergillus sulphureus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | the enzyme remains stable for 12 h at 40°C and exhibits about 30% activity after 2 h at 50°C. The enzyme shows no activity at 60°C | Aspergillus sulphureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.2 | - |
- |
Aspergillus sulphureus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 7 | more than 80% of enzyme activity is retained in the pH range 2.0-7.0 | Aspergillus sulphureus |