Information on EC 3.2.1.78 - mannan endo-1,4-beta-mannosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.78
-
RECOMMENDED NAME
GeneOntology No.
mannan endo-1,4-beta-mannosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mannan degradation
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Fructose and mannose metabolism
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SYSTEMATIC NAME
IUBMB Comments
4-beta-D-mannan mannanohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
37288-54-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
anearogenes
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Manually annotated by BRENDA team
strain Tc-12-31
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Manually annotated by BRENDA team
strain Tc-12-31
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
MRC 11624
Uniprot
Manually annotated by BRENDA team
strain MRC11624
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Manually annotated by BRENDA team
gene man5P1
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-
Manually annotated by BRENDA team
member of glycosyl hydrolase family 5
UniProt
Manually annotated by BRENDA team
gene man5XZ3
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Manually annotated by BRENDA team
stran BK01
UniProt
Manually annotated by BRENDA team
strain FTCC 5003
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain CGMCC 1416
UniProt
Manually annotated by BRENDA team
strain CGMCC1554
Uniprot
Manually annotated by BRENDA team
strain MSK-5
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Manually annotated by BRENDA team
strain N16-5
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Manually annotated by BRENDA team
strain B36
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Manually annotated by BRENDA team
gene manA
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Manually annotated by BRENDA team
KK01
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Manually annotated by BRENDA team
KU-1
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Manually annotated by BRENDA team
beta-mannanase; strain MA139
UniProt
Manually annotated by BRENDA team
isolated from soil sample of Heilongjiang Liangshui National Nature Reserve
UniProt
Manually annotated by BRENDA team
strain WY34, induced by growth on konjac powder as carbon source at 50C
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
commercial preparation
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Manually annotated by BRENDA team
Bispora sp.
strain MEY-1
UniProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Caldicellulosiruptor sp.
Rt8B.4
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Manually annotated by BRENDA team
member of glycosyl hydrolase family 5
UniProt
Manually annotated by BRENDA team
member of glycosyl hydrolase family 5
UniProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
member of glycoyl hydrolase family 26
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Rt46B.1
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Fusicoccum sp.
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Gyoerffyella speciosa
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
snail
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Manually annotated by BRENDA team
gene HhMan
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Lilium testaceum
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
i.e. Neurospora sitophila, gene man5
UniProt
Manually annotated by BRENDA team
no activity in Escherichia coli
harboring a manA-containing plasmid
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Manually annotated by BRENDA team
Orpinomyces sp.
strain PC-2
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
strain GS01, enzyme belongs to glycosyl hydrolase family 26. Protein additionally contains a glycosyl hydroxylase family 44 catalytic domain, a fibronectin domain type 3, and a cellulose-binding module type 3
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Manually annotated by BRENDA team
sequence consists of a carbohydrate binding module belonging to family 6 and a family 26 catalytic domain
UniProt
Manually annotated by BRENDA team
sequence consists of a carbohydrate binding module belonging to family 6 and a family 26 catalytic domain
UniProt
Manually annotated by BRENDA team
gene man5F63
UniProt
Manually annotated by BRENDA team
gene man5F63
UniProt
Manually annotated by BRENDA team
Pol6
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Manually annotated by BRENDA team
gene poman5A
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Manually annotated by BRENDA team
Penicillium wortmanni
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Reinekea sp. KIT-YO10
isolated from the seashore of Kanazawa Port in Japan
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Manually annotated by BRENDA team
gene RmMan5A
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Manually annotated by BRENDA team
ATCC 43812
Swissprot
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
thermophilic anaerobic bacterium
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
5068
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Manually annotated by BRENDA team
Tyromyces palustris
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
yeasts
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Automatic Mining of ENzyme DAta
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,4-beta-D-mannan + H2O
?
show the reaction diagram
2,4-dinitrophenyl-beta-mannobioside + H2O
2,4-dinitrophenol + beta-mannobiose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranose
show the reaction diagram
azo-carob galactomannan + H2O
?
show the reaction diagram
azurine-crosslinked galactomannan + H2O
?
show the reaction diagram
-
hydrolysis
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?
beta-1,4-D-mannan + H2O
?
show the reaction diagram
166% of the activity with locust bean gum
main products are manno-oligosaccharides of 2-6 residues and mannose
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?
beta-1,4-mannan + H2O
beta-1,4-mannotriose + beta-1,4-mannotetraose
show the reaction diagram
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?
birch wood xylan + H2O
?
show the reaction diagram
carboxymethyl cellulose + H2O
?
show the reaction diagram
carob galactomannan + H2O
?
show the reaction diagram
-
-
-
?
carob-galactomannan + H2O
?
show the reaction diagram
i.e. locust bean gum
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?
copra mannan + H2O
?
show the reaction diagram
-
90% of the activity with locust bean gum
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?
copra meal defatted with n-hexane + H2O
?
show the reaction diagram
galactoglucomannan + H2O
?
show the reaction diagram
galactoglucomannan + oligosaccharide
[3H]-labelled polysaccharide
show the reaction diagram
-
transglucosylation
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?
galactomannan + H2O
?
show the reaction diagram
galactomannan + H2O
galactosyl-mannosyl oligosaccharides
show the reaction diagram
galactomannan + H2O
hydrolyzed galactomannan
show the reaction diagram
galactomannan + H2O
mannose + mannobiose + mannotriose + mannotetraose + galactose-linked mannotetraose + mannopentaose + mannohexaose
show the reaction diagram
-
different contents of glucose and mannose, overview
product ratio overview
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?
glucomannan + H2O
?
show the reaction diagram
glucomannan + H2O
glucosyl-mannosyl oligosaccharides
show the reaction diagram
glucomannan + H2O
oligosaccharides
show the reaction diagram
guar gum + H2O
?
show the reaction diagram
guar gum + H2O
mannobiose + mannotriose
show the reaction diagram
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-
mannobiose, mannotriose, and a mixture of various mannose-linked oligosaccharides
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?
guar gum + H2O
mannose + mannobiose + mannotriose
show the reaction diagram
guar gum + H2O
mannose + mannobiose + mannotriose + mannotetraose + galactose-linked mannotetraose + mannopentaose + mannohexaose
show the reaction diagram
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product ratio overview
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?
guar gum galactomannan + H2O
?
show the reaction diagram
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-
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?
guar gum glucomannan + H2O
?
show the reaction diagram
ivory nut mannan + H2O
?
show the reaction diagram
ivory nut mannan + H2O
mannobiose + mannotriose
show the reaction diagram
ivory nut mannan + H2O
mannotriose + mannotetraose + mannopentaose + mannohexaose
show the reaction diagram
konjac glucomannan + H2O
?
show the reaction diagram
locust bean gum + H2O
?
show the reaction diagram
locust bean gum + H2O
mannobiose + mannotriose
show the reaction diagram
locust bean gum + H2O
mannobiose + mannotriose + mannotetraose
show the reaction diagram
locust bean gum + H2O
mannopentaose
show the reaction diagram
locust bean gum + H2O
mannose + galactose
show the reaction diagram
locust bean gum + H2O
mannose + mannobiose + mannotriose + mannotetraose + galactose-linked mannotetraose + mannopentaose + mannohexaose
show the reaction diagram
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-
product ratio overview
-
?
locust bean gum galactomannan + H2O
mannobiose
show the reaction diagram
-
main product, with low amounts of mannotriose and higher manno-oligosaccharides
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?
locust bean gum glucomannan + H2O
?
show the reaction diagram
low viscosity locust bean galactomannan + H2O
?
show the reaction diagram
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-
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?
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O
2 Man-beta-1,4-Man
show the reaction diagram
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O
Man-beta-1,4-Man-beta-1,4-Man + Man-beta-1,4-Man
show the reaction diagram
Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man + H2O
Man-beta-1,4-Man + Man-beta-1,4-Man-beta-1,4-Man-beta-1,4-Man
show the reaction diagram
Manbeta(1-4)Manbeta(1-4)Man + H2O
?
show the reaction diagram
-
-
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?
Manbeta(1-4)Manbeta(1-4)Man + H2O
mannose + Manbeta(1-4)Man
show the reaction diagram
-
-
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?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + mannose
show the reaction diagram
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-
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?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
Manbeta(1-4)Manbeta(1-4)Man + mannose + Manbeta(1-4)Man
show the reaction diagram
-
-
-
?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
mannose + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Man
show the reaction diagram
-
-
-
-
?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man
show the reaction diagram
-
-
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-
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + mannose
show the reaction diagram
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + H2O
D-mannitol + Manbeta(1-4)Man-ol + D-mannose + Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Manbeta(1-4)Man
show the reaction diagram
-
-
-
-
?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + H2O
Manbeta(1-4)Man-ol + Manbeta(1-4)Manbeta(1-4)Man + mannose + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Man
show the reaction diagram
-
-
-
?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + mannose
show the reaction diagram
-
-
-
?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Man + mannose + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
show the reaction diagram
-
-
-
-
?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + H2O
mannose + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man-ol + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
show the reaction diagram
-
-
-
?
mannan + H2O
?
show the reaction diagram
mannan + H2O
mannosyl oligosaccharides
show the reaction diagram
-
products show a degree of polymerization of 2-6
-
?
mannan + H2O
mannotetraose + mannobiose + mannotriose
show the reaction diagram
mannan + H2O
oligosaccharides
show the reaction diagram
mannan A + H2O
?
show the reaction diagram
-
-
-
-
?
mannohexaose + H2O
?
show the reaction diagram
-
-
-
-
?
mannohexaose + H2O
mannotriose
show the reaction diagram
-
-
main product
-
?
mannopentaose + H2O
?
show the reaction diagram
mannopentaose + H2O
mannose + mannobiose + mannotriose + mannotetraose
show the reaction diagram
mannopentaose + H2O
mannose + mannotetraose
show the reaction diagram
-
-
-
-
?
mannopentaose + H2O
mannotriose + mannobiose
show the reaction diagram
-
-
main products
-
?
mannotetraose + H2O
?
show the reaction diagram
mannotetraose + H2O
mannobiose + mannose + mannotriose
show the reaction diagram
mannotriose + H2O
?
show the reaction diagram
mannotriose + H2O
mannobiose + mannose
show the reaction diagram
palm kernel cake + H2O
?
show the reaction diagram
starch + H2O
?
show the reaction diagram
tara gum galactomannan + H2O
?
show the reaction diagram
-
-
-
-
?
xylan + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
guar gum + H2O
mannose + mannobiose + mannotriose
show the reaction diagram
ivory nut mannan + H2O
mannobiose + mannotriose
show the reaction diagram
konjac glucomannan + H2O
?
show the reaction diagram
Reinekea sp. KIT-YO10
-
-
-
-
?
locust bean gum + H2O
mannose + galactose
show the reaction diagram
mannan + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ag+
Bispora sp.
stimulation
EDTA
64% residual activity
Fe2+
-
1 mM, 124% of initial activity
Fe3+
Bispora sp.
stimulation
Li+
1 mM, 112% of initial activity
NaCl
activates 150% at 100 mg/ml
Ni2+
Bispora sp.
stimulation
Pb2+
slight stimulation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
1 mM, 54% residual activity
4-nitrophenyl-thio-beta-D-mannopyranoside
-
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abscisic acid
-
presence of exogenous abscisic acid provokes a delay in the cellular disassembly of the endosperm and disappearance of endo-beta-mannanase in the tissue, resulting in delayed galactomannan degradation
dithiothreitol
-
1 mM, 25% loss of activity
N-bromosuccinimide
n-butanol
-
complete inhibition at 20-30% v/v
p-chloromercuribenzoate
-
-
Sodium dodecyl sulfate
sodium dodecylsulfate
Triton X-100
-
-
additional information
-