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Literature summary for 3.2.1.78 extracted from
- Secretory expression and characterization of a novel thermo-stable, salt-tolerant endo-1,4-beta-mannanase of Bacillus subtilis WD23 by Pichia pastoris (2015), Eur. Food Res. Technol., 240, 671-677.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning in Escherichia coli strain JM109, functional overexpression in Pichia pastoris strain GS115, the enzyme is secreted | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | - |
Bacillus subtilis |  |
| Cu2+ | - |
Bacillus subtilis | |
| EDTA | - |
Bacillus subtilis | |
| Fe2+ | - |
Bacillus subtilis | |
| Fe3+ | - |
Bacillus subtilis | |
| Hg2+ | - |
Bacillus subtilis | |
| SDS | - |
Bacillus subtilis | |
| Zn2+ | - |
Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Bacillus subtilis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Bacillus subtilis | |
| additional information | Na+, Li+, NH4+, Ca2+, Mg2+ and Ba2+ have no effect on the recombinant mannanase activity | Bacillus subtilis | |
| NaCl | activates 150% at 100 mg/ml | Bacillus subtilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | D0UH99 | isolated from soil sample of Heilongjiang Liangshui National Nature Reserve | - |
| Bacillus subtilis WD23 | D0UH99 | isolated from soil sample of Heilongjiang Liangshui National Nature Reserve | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 9.7fold from Pichia pastoris strain GS115 culture supernatant to homogeneity by ammonium sulfate fractionation, dialysis, PEG 20000 fractionation, anion exchange chromatography, ultrafiltration, and gel filtration | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4449 | - |
purified recombinant enzyme, pH 6.3, 70°C | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-1,4-beta-mannanase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
recombinant enzyme | Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | recombinant enzyme, over 80% of maximal activity within this range | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
recombinant enzyme | Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 9.1 | recombinant enzyme, more than 80% of the maximum activity at pH 4.8-7.8, the enzyme activity drops rapidly when at below pH 4.8 or above pH 9.1 | Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.8 | purified recombinant enzyme, over 80% remaning after 1 h | Bacillus subtilis |
| 6.3 | 7.1 | purified recombinant enzyme, over 80% remaning after 2 h | Bacillus subtilis |
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