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Literature summary for 3.2.1.78 extracted from
- A novel beta-mannanase with high specific activity from Bacillus circulans CGMCC1554: gene cloning, expression and enzymatic characterization (2008), Appl. Biochem. Biotechnol., 159, 85-94.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris and Escherichia coli | Niallia circulans |
General Stability
| General Stability | Organism |
|---|---|
| enzyme is resistant to digestion by trypsin | Niallia circulans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | strong inhibition | Niallia circulans |  |
| Hg2+ | strong inhibition | Niallia circulans | |
| Sodium dodecyl sulfate | strong inhibition | Niallia circulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, calculated and SDS-PAGE | Niallia circulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | Q0IJR6 | - |
- |
| Niallia circulans CGMCC1554 | Q0IJR6 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4839 | - |
pH 7.6, 60°C | Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| locust bean gum + H2O | - |
Niallia circulans | ? | 76% of products are oligomannosides | ? | |
| locust bean gum + H2O | - |
Niallia circulans CGMCC1554 | ? | 76% of products are oligomannosides | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, calculated and SDS-PAGE | Niallia circulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Niallia circulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
60 min, 90% residual activity | Niallia circulans |
| 60 | - |
20 min, complete loss of activity | Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Niallia circulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | more than 75% of maximum activity within | Niallia circulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | retains more than 75% of maximum activity after 1 h incubation | Niallia circulans |
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