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Literature summary for 3.2.1.78 extracted from

  • Kim, d.o..Y.; Ham, S.J.; Lee, H.J.; Cho, H.Y.; Kim, J.H.; Kim, Y.J.; Shin, D.H.; Rhee, Y.H.; Son, K.H.; Park, H.Y.
    Cloning and characterization of a modular GH5 beta-1,4-mannanase with high specific activity from the fibrolytic bacterium Cellulosimicrobium sp. strain HY-13 (2011), Biores. Technol., 102, 9185-9192.
    View publication on PubMed
Search for more literature for 3.2.1.78

Activating Compound

Activating Compound Comment Organism Structure
Sodium azide hydrolysis of locust bean gum is enhanced by approximately 1.45fold in poresence of sodium azide Cellulosimicrobium sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cellulosimicrobium sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ more than 50% inhibition Cellulosimicrobium sp.
EDTA 5 mM, 20% residaul activity Cellulosimicrobium sp.
Fe2+ more than 50% inhibition Cellulosimicrobium sp.
Zn2+ more than 50% inhibition Cellulosimicrobium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43767
-
 x * 44000, SDS-PAGE, x * 43767, calculated Cellulosimicrobium sp.
44000
-
 x * 44000, SDS-PAGE, x * 43767, calculated Cellulosimicrobium sp.

Organism

Organism UniProt Comment Textmining
Cellulosimicrobium sp. G3FBL1 member of glycosyl hydrolase family 5
-
Cellulosimicrobium sp. HY-13 G3FBL1 member of glycosyl hydrolase family 5
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a N-terminal signal peptide of 24 amino acids Cellulosimicrobium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8498
-
 substrate locust bean gum, pH 6.0, 50°C Cellulosimicrobium sp.
14710
-
 substrate ivory nut mannan, pH 6.0, 50°C Cellulosimicrobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ivory nut mannan + H2O
-
 Cellulosimicrobium sp. mannotriose + mannotetraose + mannopentaose + mannohexaose main products, at 14%, 25%, 20%, and 13%, respectively ?
ivory nut mannan + H2O
-
 Cellulosimicrobium sp. HY-13 mannotriose + mannotetraose + mannopentaose + mannohexaose main products, at 14%, 25%, 20%, and 13%, respectively ?
locust bean gum + H2O
-
 Cellulosimicrobium sp. mannopentaose 44%, main product ?
locust bean gum + H2O
-
 Cellulosimicrobium sp. HY-13 mannopentaose 44%, main product ?
additional information no substrate: mannobiose, or 4-nitrophenyl sugar derivatives Cellulosimicrobium sp. ?
-
 ?
additional information no substrate: mannobiose, or 4-nitrophenyl sugar derivatives Cellulosimicrobium sp. HY-13 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE, x * 43767, calculated Cellulosimicrobium sp.

Synonyms

Synonyms Comment Organism
ManH
-
 Cellulosimicrobium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Cellulosimicrobium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life 15 min Cellulosimicrobium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Cellulosimicrobium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 1 h, more than 80% residual activity Cellulosimicrobium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Cellulosimicrobium sp. calculated
-
 4.6

General Information

General Information Comment Organism
physiological function enzyme strongly binds to ivory nut mannan, Avicel, chitosan, and chitin, but does not attach to curdlan, insoluble oat spelt xylan, lignin, or poly(3-hydroxybutyrate) Cellulosimicrobium sp.