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Literature summary for 3.2.1.78 extracted from

  • Li, Y.N.; Meng, K.; Wang, Y.R.; Yao, B.
    A beta-mannanase from Bacillus subtilis B36: purification, properties, sequencing, gene cloning and expression in Escherichia coli (2007), Z. Naturforsch. C, 61, 840-846.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 3% residual activity Bacillus subtilis
Hg2+ 1 mM, 2% residual activity Bacillus subtilis
additional information not inhibitory: EDTA, 2-mercaptoethanol Bacillus subtilis
sodium dodecylsulfate 1 mM, 79% residual activity Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus subtilis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
x * 38013, calculated, x * 38000, SDS-PAGE, mature protein Bacillus subtilis
38013
-
x * 38013, calculated, x * 38000, SDS-PAGE, mature protein Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis B36
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from culture supernatant Bacillus subtilis

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
Bacillus subtilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
928
-
pH 6.4, 50°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guar gum + H2O 21% of the activity with locust bean gum Bacillus subtilis ?
-
?
guar gum + H2O 21% of the activity with locust bean gum Bacillus subtilis B36 ?
-
?
locust bean gum + H2O enzyme is highly specific for substrate Bacillus subtilis ?
-
?
locust bean gum + H2O enzyme is highly specific for substrate Bacillus subtilis B36 ?
-
?
additional information no substrate: carboxymethyl cellulose Bacillus subtilis ?
-
?
additional information no substrate: carboxymethyl cellulose Bacillus subtilis B36 ?
-
?

Subunits

Subunits Comment Organism
? x * 38013, calculated, x * 38000, SDS-PAGE, mature protein Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
60 min, 90% residual activity Bacillus subtilis
70
-
30 min, 20% residual activity Bacillus subtilis
80
-
20 min, 18% residual activity, 30 min, complete loss of activity Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
5 8 more than 70% of maximum activity Bacillus subtilis
10
-
44% of maximum activity Bacillus subtilis