Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 3% residual activity | Bacillus subtilis | |
Hg2+ | 1 mM, 2% residual activity | Bacillus subtilis | |
additional information | not inhibitory: EDTA, 2-mercaptoethanol | Bacillus subtilis | |
sodium dodecylsulfate | 1 mM, 79% residual activity | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38013, calculated, x * 38000, SDS-PAGE, mature protein | Bacillus subtilis |
38013 | - |
x * 38013, calculated, x * 38000, SDS-PAGE, mature protein | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis B36 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from culture supernatant | Bacillus subtilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture fluid | - |
Bacillus subtilis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
928 | - |
pH 6.4, 50°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guar gum + H2O | 21% of the activity with locust bean gum | Bacillus subtilis | ? | - |
? | |
guar gum + H2O | 21% of the activity with locust bean gum | Bacillus subtilis B36 | ? | - |
? | |
locust bean gum + H2O | enzyme is highly specific for substrate | Bacillus subtilis | ? | - |
? | |
locust bean gum + H2O | enzyme is highly specific for substrate | Bacillus subtilis B36 | ? | - |
? | |
additional information | no substrate: carboxymethyl cellulose | Bacillus subtilis | ? | - |
? | |
additional information | no substrate: carboxymethyl cellulose | Bacillus subtilis B36 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38013, calculated, x * 38000, SDS-PAGE, mature protein | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
60 min, 90% residual activity | Bacillus subtilis |
70 | - |
30 min, 20% residual activity | Bacillus subtilis |
80 | - |
20 min, 18% residual activity, 30 min, complete loss of activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | more than 70% of maximum activity | Bacillus subtilis |
10 | - |
44% of maximum activity | Bacillus subtilis |