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Literature summary for 3.2.1.78 extracted from

  • Cho, K.M.; Hong, S.Y.; Lee, S.M.; Kim, Y.H.; Kahng, G.G.; Kim, H.; Yun, H.D.
    A cel44C-man26A gene of endophytic Paenibacillus polymyxa GS01 has multi-glycosyl hydrolases in two catalytic domains (2006), Appl. Microbiol. Biotechnol., 73, 618-630.
    View publication on PubMed
Search for more literature for 3.2.1.78

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paenibacillus polymyxa

Protein Variants

Protein Variants Comment Organism
E222A no catalytic acitivity Paenibacillus polymyxa
E91A no catalytic acitivity Paenibacillus polymyxa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145294
-
 x * 145294, calculated Paenibacillus polymyxa

Organism

Organism UniProt Comment Textmining
Paenibacillus polymyxa
-
 enzyme belongs to glycosyl hydrolase family 26. Protein additionally contains a glycosyl hydroxylase family 44 catalytic domain, a fibronectin domain type 3, and a cellulose-binding module type 3
-
Paenibacillus polymyxa GS01
-
 enzyme belongs to glycosyl hydrolase family 26. Protein additionally contains a glycosyl hydroxylase family 44 catalytic domain, a fibronectin domain type 3, and a cellulose-binding module type 3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Paenibacillus polymyxa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
locust bean gum + H2O
-
 Paenibacillus polymyxa ?
-
 ?
locust bean gum + H2O
-
 Paenibacillus polymyxa GS01 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 145294, calculated Paenibacillus polymyxa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Paenibacillus polymyxa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 xylanase and mannanase activities of enzyme Paenibacillus polymyxa

pI Value

Organism Comment pI Value Maximum pI Value
Paenibacillus polymyxa calculated
-
 5.5