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Literature summary for 3.2.1.78 extracted from

  • Guo, N.; Zheng, J.; Wu, L.; Tian, J.; Zhou, H.
    Engineered bifunctional enzymes of endo-1,4-beta-xylanase/endo-1,4-beta-mannanase were constructed for synergistically hydrolyzing hemicellulose (2013), J. Mol. Catal. B, 97, 311-318.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene An15g07760, cloning in Escherichia coli, and expression of recombinant chimeric xylanase/mannanase fusion enzymes in Pichia pastoris strain X33 using GAP promoter, secretion to the medium Aspergillus niger

Protein Variants

Protein Variants Comment Organism
additional information construction of a chimeric enzyme of xylanase and mannanase with higher catalytic efficiency and improved properties, the two enzymes are fused together with twelve fusion types Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of recombinant chimeric enzymes, overview Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger A2R6F5 gene An15g07760
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Aspergillus niger CBS 513.88 A2R6F5 gene An15g07760
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Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein deglycosylation by endo-beta-N-acetylglucosaminidase H Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
recombinant chimeric enzymes from Pichia pastoris strain X33 culture supernatant by ammonium sulfate fractionation and gel filtration Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
768.5 1249 purified recombinant chimeric enzymes, pH 5.0, 50°C Aspergillus niger

Synonyms

Synonyms Comment Organism
Beta-mannanase
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Aspergillus niger
endo-1,4-beta-mannanase
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Aspergillus niger
Man
-
Aspergillus niger
mannan endo-1,4-beta-mannanase
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 55 recombinant chimeric enzymes Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 80 activity range, recombinant chimeric enzymes, profiles overview Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 80 varying residual activities of recombinant chimeric enzymes, overview Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
recombinant chimeric enzymes Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
2 7 activity range, recombinant chimeric enzymes, profiles overview Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.2 11 purified recombinant chimeric enzymes display similar high pH stability as the wild-type mannanase with over 80% activity in this range, profiles overview Aspergillus niger