Literature summary for 3.2.1.78 extracted from
Guo, N.; Zheng, J.; Wu, L.; Tian, J.; Zhou, H.
Engineered bifunctional enzymes of endo-1,4-beta-xylanase/endo-1,4-beta-mannanase were constructed for synergistically hydrolyzing hemicellulose (2013), J. Mol. Catal. B, 97, 311-318.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
gene An15g07760, cloning in Escherichia coli, and expression of recombinant chimeric xylanase/mannanase fusion enzymes in Pichia pastoris strain X33 using GAP promoter, secretion to the medium |
Aspergillus niger |
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
construction of a chimeric enzyme of xylanase and mannanase with higher catalytic efficiency and improved properties, the two enzymes are fused together with twelve fusion types |
Aspergillus niger |
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
additional information |
- |
additional information |
kinetics of recombinant chimeric enzymes, overview |
Aspergillus niger |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Aspergillus niger |
A2R6F5 |
gene An15g07760 |
- |
Aspergillus niger CBS 513.88 |
A2R6F5 |
gene An15g07760 |
- |
Posttranslational Modification
Posttranslational Modification |
Comment |
Organism |
glycoprotein |
deglycosylation by endo-beta-N-acetylglucosaminidase H |
Aspergillus niger |
Purification (Commentary)
Purification (Comment) |
Organism |
recombinant chimeric enzymes from Pichia pastoris strain X33 culture supernatant by ammonium sulfate fractionation and gel filtration |
Aspergillus niger |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
768.5 |
1249 |
purified recombinant chimeric enzymes, pH 5.0, 50°C |
Aspergillus niger |
Synonyms
Synonyms |
Comment |
Organism |
Beta-mannanase |
- |
Aspergillus niger |
endo-1,4-beta-mannanase |
- |
Aspergillus niger |
Man |
- |
Aspergillus niger |
mannan endo-1,4-beta-mannanase |
- |
Aspergillus niger |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
45 |
55 |
recombinant chimeric enzymes |
Aspergillus niger |
Temperature Range [°C]
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
10 |
80 |
activity range, recombinant chimeric enzymes, profiles overview |
Aspergillus niger |
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
10 |
80 |
varying residual activities of recombinant chimeric enzymes, overview |
Aspergillus niger |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
5 |
- |
recombinant chimeric enzymes |
Aspergillus niger |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
2 |
7 |
activity range, recombinant chimeric enzymes, profiles overview |
Aspergillus niger |
pH Stability
pH Stability |
pH Stability Maximum |
Comment |
Organism |
2.2 |
11 |
purified recombinant chimeric enzymes display similar high pH stability as the wild-type mannanase with over 80% activity in this range, profiles overview |
Aspergillus niger |