Application | Comment | Organism |
---|---|---|
industry | the enzyme is highly useful in the food/feed, paper and pulp, and detergent industries | Rhizomucor miehei |
Cloned (Comment) | Organism |
---|---|
gene RmMan5A, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged enzyme in Escherichia coli strain BL21 | Rhizomucor miehei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values of the recombinant enzyme are 3.78 mg/ml for locust bean gum, and 7.75 mg/ml for guar gum | Rhizomucor miehei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, recombinant enzyme, SDS-PAGE | Rhizomucor miehei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizomucor miehei | - |
gene RmMan5A | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Rhizomucor miehei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10760 | - |
purified recombinant enzyme, pH 7.0, 55°C, substrate locust bean gum | Rhizomucor miehei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guar gum + H2O | - |
Rhizomucor miehei | mannobiose + mannotriose | mannobiose, mannotriose, and a mixture of various mannose-linked oligosaccharides | ? | |
locust bean gum + H2O | best substrate | Rhizomucor miehei | mannobiose + mannotriose | mannobiose, mannotriose, and a mixture of various mannose-linked oligosaccharides | ? | |
additional information | the enzyme shows requirement of four mannose residues for hydrolysis, and is also capable of catalyzing transglycosylation reactions | Rhizomucor miehei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, recombinant enzyme, SDS-PAGE | Rhizomucor miehei |
Synonyms | Comment | Organism |
---|---|---|
endo-1,4-beta-mannanase | - |
Rhizomucor miehei |
endo-acting beta-1,4-mannanase | - |
Rhizomucor miehei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Rhizomucor miehei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | activity range, recombinant enzyme, profile overview | Rhizomucor miehei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
purified recombinant enzyme, without substrate, stable up to, 90% activity remaning at 55°C after 30 min | Rhizomucor miehei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.77 | - |
guar gum | pH 7.0, 55°C, recombinant enzyme | Rhizomucor miehei | |
14.9 | - |
locust bean gum | pH 7.0, 55°C, recombinant enzyme | Rhizomucor miehei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhizomucor miehei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | activity range, recombinant enzyme, profile overview | Rhizomucor miehei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | purified recombinant enzyme, 30 min, without substrate, over 80% activity remaining | Rhizomucor miehei |