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Literature summary for 3.2.1.78 extracted from

  • Katrolia, P.; Yan, Q.; Zhang, P.; Zhou, P.; Yang, S.; Jiang, Z.
    Gene cloning and enzymatic characterization of an alkali-tolerant endo-1,4-beta-mannanase from Rhizomucor miehei (2013), J. Agric. Food Chem., 61, 394-401.
    View publication on PubMed

Application

Application Comment Organism
industry the enzyme is highly useful in the food/feed, paper and pulp, and detergent industries Rhizomucor miehei

Cloned(Commentary)

Cloned (Comment) Organism
gene RmMan5A, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His-tagged enzyme in Escherichia coli strain BL21 Rhizomucor miehei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km values of the recombinant enzyme are 3.78 mg/ml for locust bean gum, and 7.75 mg/ml for guar gum Rhizomucor miehei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, recombinant enzyme, SDS-PAGE Rhizomucor miehei

Organism

Organism UniProt Comment Textmining
Rhizomucor miehei
-
gene RmMan5A
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Rhizomucor miehei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10760
-
purified recombinant enzyme, pH 7.0, 55°C, substrate locust bean gum Rhizomucor miehei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guar gum + H2O
-
Rhizomucor miehei mannobiose + mannotriose mannobiose, mannotriose, and a mixture of various mannose-linked oligosaccharides ?
locust bean gum + H2O best substrate Rhizomucor miehei mannobiose + mannotriose mannobiose, mannotriose, and a mixture of various mannose-linked oligosaccharides ?
additional information the enzyme shows requirement of four mannose residues for hydrolysis, and is also capable of catalyzing transglycosylation reactions Rhizomucor miehei ?
-
?

Subunits

Subunits Comment Organism
? x * 43000, recombinant enzyme, SDS-PAGE Rhizomucor miehei

Synonyms

Synonyms Comment Organism
endo-1,4-beta-mannanase
-
Rhizomucor miehei
endo-acting beta-1,4-mannanase
-
Rhizomucor miehei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Rhizomucor miehei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 activity range, recombinant enzyme, profile overview Rhizomucor miehei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
purified recombinant enzyme, without substrate, stable up to, 90% activity remaning at 55°C after 30 min Rhizomucor miehei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.77
-
guar gum pH 7.0, 55°C, recombinant enzyme Rhizomucor miehei
14.9
-
locust bean gum pH 7.0, 55°C, recombinant enzyme Rhizomucor miehei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Rhizomucor miehei

pH Range

pH Minimum pH Maximum Comment Organism
4 10 activity range, recombinant enzyme, profile overview Rhizomucor miehei

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 purified recombinant enzyme, 30 min, without substrate, over 80% activity remaining Rhizomucor miehei