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3.2.1.35: hyaluronoglucosaminidase

This is an abbreviated version!
For detailed information about hyaluronoglucosaminidase, go to the full flat file.

Word Map on EC 3.2.1.35

Reaction

hyaluronic acid hexasaccharide
+
H2O
=
hyaluronic acid disaccharide
 +
hyaluronic acid tetrasaccharide

Synonyms

BmHYA1, BPH-20, BTH, BVH, chondritinase I, chondroitin sulfate hydrolase, chondroitinase, chondroitinase I, Cumulase, Dietrich's hyaluronidase, EC 3.2.1.34, endo-alpha-N-acetyl-hexosaminidase, endo-beta-N-acetyl-D-hexosaminidases hydrolase, endo-beta-N-acetylhexosaminidase, HAase, HPH-20, HU, human PH-20, Hya, Hya-1, Hya1, Hyal, Hyal 1, HYAL 2, HYAL 3, HYAL 4, Hyal-1, Hyal-2, Hyal-3, Hyal-4, Hyal-Ba, HYAL1, HYAL1-v1, HYAL1-v3, Hyal2, HYAL3, HYAL3-v1, HYAL3-v2, HYAL4, HYAL5, hyaluronate 4-glycanohydrolase, hyaluronidase, hyaluronidase 1, hyaluronidase 2, hyaluronidase 3, hyaluronidase PH20, hyaluronidase-1, hyaluronidase-2, hyaluronidase-4, hyaluronidase-5, hyaluronoglucosaminidase, hyaluronoglucosaminidase 1, Hyaluronoglucosaminidase 1-type hyaluronidase, hyaluronoglucosaminidase 2, hyaluronoglucosidase, HYase, HYL, hylB, jaagsiekte sheep retrovirus receptor, LUCA-1, LUCA-2, Luca-3, LUCA2, MGEA5, Mu toxin, Neopermease, NNH1, NNH2, OTH, PH-20, rHuPH20, SFHYA1, SPAM1, sperm hyaluronidase 1, Sperm surface protein PH-20, spreading factor, testicular HAse, testicular hyaluronidase, vitrase, XKH1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.35 hyaluronoglucosaminidase

Purification

Purification on EC 3.2.1.35 - hyaluronoglucosaminidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
25.6fold to homogeneity from venom by gel filtration and ion exchange chromatography
Palamneus gravimanus
-
according to their inhibition activity for hyaluronidases, 6-chloromethyluracil and 2-aminobenzimidazole are coupled with sepharose beads for the affinity adsorption of bovine hyaluronidase-1. In good accordance with structural simulation, the ligands designed from 2-aminobenzimidazole show much higher specificity for the target enzyme, because they had more H bonds and pi-pi interactions with target sites. After optimization, through one step of affinity adsorption with sorbent L-4, bovine hyaluronidase can be isolated from the supernatant of bovine testes extract, with purity and recovery up to 96.4% and 89.4%, respectively. Based on the less steps of purification, short time consuming, higher purity, and higher activity recoveries, industrial application of this method is of great potential
ammonium sulfate precipitation and Ni-ion metal affinity chromatography
-
by ion exchange chromatography at pH 5.0
Chelating Sepharose Fast Flow column chromatography and Sephacryl S200 gel filtration
CM-cellulose-52 column chromatography, phenyl-Sepharose column chromatography, and Sephacryl S-100 gel filtration
commercial product
-
from sperm surface by treatment with phosphatidylinositol-specific phospholipase C
-
further purification of the commercial preparation
-
heparin-Sepharose column chromatography
-
HiTrap HP SP column chromatography
HiTrap Q column chromatography and Superdex 200 HR 10/30 gel filtration
-
Hyal2 is strongly associated with cell membrane fractions from which it can be extracted using a Triton X-114 treatment, but not an osmotic shock or an alkaline carbonate solution. Treatment of membrane preparations with phosphatidylinositol-specific phospholipase C releases immunoreactive Hyal2 into the aqueous phase. The protein is attached to the membrane through a functional GPI anchor. Hyal2 transfected in COS-7 cells is associated with detergent-resistant, cholesterol-rich membranes known as lipid rafts
isozymes NNH1 and NNH2 33fold and 26fold, respectively, from venom by gel filtration and ion exchange chromatography
-
native enzyme from lyophilized venom by affinity chromatography
native isozyme NNH1 33fold to homogeneity from venom by successive gel filtration and ion exchange chromatography
-
native isozyme NNH2 26fold to homogeneity from venom by successive gel filtration and ion exchange chromatography
-
partial
purified by gel filtration on Sephacryl S-100 and ion-exchange chromatography on SP-Sepharose. Procedure provides 376.4fold purification with a 2.94% yield
-
recombinant His-tagged enzyme
recombinant His6-tagged viral enzyme from insect cells by metal affinity chromatography, ultrafiltration, and gel filtration
-
recombinant protein
Sephadex G-100 column chromatography and CM-Sephadex C-25 column chromatography
-
Sephadex G-50 gel filtration
SP Sepharose column chromatography and Sephacryl S-100 gel filtration
-
to homogeneity, 2step chromatography
to homogeneity, chromatography steps
-
to homogeneity, chromatography, isoelectric focusing
-
using successive chromatography: gel filtration, anion exchange chromatography, cation exchange chromatography, reversed-phase HPLC