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Literature summary for 3.2.1.35 extracted from

  • Li, M.W; Yudin, A.I.; Robertson, K.R.; Cherr, G.N.; Overstreet, J.W.
    Importance of glycosylation and disulfide bonds in hyaluronidase activity of macaque sperm surface PH-20 (2002), J. Androl., 23, 211-219.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Macaca sp.
DTT
-
Macaca sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane inner acrosomal membrane Macaca sp. 16020
-
plasma membrane cell surface Macaca sp. 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
x * 64000, SDS-PAGE Macaca sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hyaluronic acid-rich cumulus matrix + H2O Macaca sp. investment of oocyte during sperm invasion ?
-
?

Organism

Organism UniProt Comment Textmining
Macaca sp.
-
6 isozymes in sperm
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylation is absolutely necessary for activity, deglycosylation leads to complete loss of activity, enzyme binds to lectins Con A, LEL, STL, PHA-e, Jacalin, and PSA, mannose is the major sugar within and at the terminal sequence of the glycans Macaca sp.

Purification (Commentary)

Purification (Comment) Organism
from sperm surface by treatment with phosphatidylinositol-specific phospholipase C Macaca sp.

Source Tissue

Source Tissue Comment Organism Textmining
sperm
-
Macaca sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronic acid-rich cumulus matrix + H2O
-
Macaca sp. ?
-
?
hyaluronic acid-rich cumulus matrix + H2O investment of oocyte during sperm invasion Macaca sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Macaca sp.

Synonyms

Synonyms Comment Organism
hyaluronidase
-
Macaca sp.
PH-20
-
Macaca sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Macaca sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Macaca sp.

pI Value

Organism Comment pI Value Maximum pI Value
Macaca sp. isoelectric focusing, 6 isoforms
-
5.1