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3.2.1.35: hyaluronoglucosaminidase

This is an abbreviated version!
For detailed information about hyaluronoglucosaminidase, go to the full flat file.

Word Map on EC 3.2.1.35

Reaction

hyaluronic acid hexasaccharide
+
H2O
=
hyaluronic acid disaccharide
 +
hyaluronic acid tetrasaccharide

Synonyms

BmHYA1, BPH-20, BTH, BVH, chondritinase I, chondroitin sulfate hydrolase, chondroitinase, chondroitinase I, Cumulase, Dietrich's hyaluronidase, EC 3.2.1.34, endo-alpha-N-acetyl-hexosaminidase, endo-beta-N-acetyl-D-hexosaminidases hydrolase, endo-beta-N-acetylhexosaminidase, HAase, HPH-20, HU, human PH-20, Hya, Hya-1, Hya1, Hyal, Hyal 1, HYAL 2, HYAL 3, HYAL 4, Hyal-1, Hyal-2, Hyal-3, Hyal-4, Hyal-Ba, HYAL1, HYAL1-v1, HYAL1-v3, Hyal2, HYAL3, HYAL3-v1, HYAL3-v2, HYAL4, HYAL5, hyaluronate 4-glycanohydrolase, hyaluronidase, hyaluronidase 1, hyaluronidase 2, hyaluronidase 3, hyaluronidase PH20, hyaluronidase-1, hyaluronidase-2, hyaluronidase-4, hyaluronidase-5, hyaluronoglucosaminidase, hyaluronoglucosaminidase 1, Hyaluronoglucosaminidase 1-type hyaluronidase, hyaluronoglucosaminidase 2, hyaluronoglucosidase, HYase, HYL, hylB, jaagsiekte sheep retrovirus receptor, LUCA-1, LUCA-2, Luca-3, LUCA2, MGEA5, Mu toxin, Neopermease, NNH1, NNH2, OTH, PH-20, rHuPH20, SFHYA1, SPAM1, sperm hyaluronidase 1, Sperm surface protein PH-20, spreading factor, testicular HAse, testicular hyaluronidase, vitrase, XKH1

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.35 hyaluronoglucosaminidase

Cloned

Cloned on EC 3.2.1.35 - hyaluronoglucosaminidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of core nucleotide sequence in two fused fragments, PH-20 gene DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
DNA and amino acid sequence analysis, expression as active C-terminally His-tagged enzyme in insect cells via baculovirus infection
DNA and amino acid sequence determination and analysis, expressed by injection of mRNA in frog oocytes on the outer surface of the cell membrane
DNA and amino acid sequence determination and analysis, in vitro translation
expressed in 22Rv1 human prostate adenomacarcinoma cells and in HEK-293 human embryonic kidney cells as a N-terminal FLAG fusion protein. Abundant soluble FLAG-tagged Hyal1 is secreted to the conditioned cell culture media of transfectants
expressed in baby hamster kidney cells
expressed in COS-7 cells
expressed in Drosophila melanogaster Schneider 2 cells
expressed in Escherichia coli and in Xenopus laevis oocytes
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli BL21(DE3)RIL cells and Drosophila melanogaster Schneider-2 cells
-
expressed in Pichia pastoris
-
expressed in Pichia pastoris strain GS115
expressed in S-2 cells
-
expression in Escherichia coli
expression in human bronchial epithelial cell (HBE)
expression in Pichia pastoris GS115, expression vector pPIC9, together with human serum albumin, expression in Escherichia coli BL21 DE3, vector pMW172, fusion protein
expression of His6-tagged viral enzyme via baculovirus transfection in Spodoptera frugiperda Sf9 insect cells, the His-tag replaces the GPI anchor of the enzyme to make it soluble
-
gene mgea5, DNA sequence determination and analysis, genomic organization on chromosome 10, 2 splice variants, one missing a putative acetyltransferase domain of the enzyme
genes HYAL1, HYAL2, and HYAL3 are clustered on chromosome SSC13q21, DNA and amino acid sequence determination and analysis
genes HYAL1, HYAL2, and HYAL3 clustered on chromosome SSC13q21, DNA and amino acid sequence determination and analysis
isozymes are clustered on chromosome 9F1-F2 and 6A2, expression profiles
-
isozymes Hyal1-Hyal4, sequence comparison, the genes encoding isozymes Hyal1-Hyal3 are tightly clustered on chromosome 3q21.3, Hyal4 and HPH-20 are clustered on 7q31.3, expression profiles
-
precursor from venom gland, DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence comparisons, functional expression of His-tagged enzyme in insect cells, but not in Escherichia coli, and secretion of the recombinant enzyme, overview
the bovine SPAM1 open reading frame is cloned into the mammalian expression vector pCXN2 and then transfected to the HEK293 cell line
transfection of COS-7 cell
transient expression in Nicotiana benthamiana
using the information of preferred Pichia pastoris codons as provided by Genscript, Inc., the native sequence is de novo synthesized to create a fully codon-optimized cDNA (Hya delta c) and the synthetic gene is expressed in Pichia
EU152302