EC Number |
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3.2.1.35 | - |
3.2.1.35 | 25.6fold to homogeneity from venom by gel filtration and ion exchange chromatography |
3.2.1.35 | according to their inhibition activity for hyaluronidases, 6-chloromethyluracil and 2-aminobenzimidazole are coupled with sepharose beads for the affinity adsorption of bovine hyaluronidase-1. In good accordance with structural simulation, the ligands designed from 2-aminobenzimidazole show much higher specificity for the target enzyme, because they had more H bonds and pi-pi interactions with target sites. After optimization, through one step of affinity adsorption with sorbent L-4, bovine hyaluronidase can be isolated from the supernatant of bovine testes extract, with purity and recovery up to 96.4% and 89.4%, respectively. Based on the less steps of purification, short time consuming, higher purity, and higher activity recoveries, industrial application of this method is of great potential |
3.2.1.35 | ammonium sulfate precipitation and Ni-ion metal affinity chromatography |
3.2.1.35 | by ion exchange chromatography at pH 5.0 |
3.2.1.35 | Chelating Sepharose Fast Flow column chromatography and Sephacryl S200 gel filtration |
3.2.1.35 | CM-cellulose-52 column chromatography, phenyl-Sepharose column chromatography, and Sephacryl S-100 gel filtration |
3.2.1.35 | commercial product |
3.2.1.35 | from sperm surface by treatment with phosphatidylinositol-specific phospholipase C |
3.2.1.35 | from venom |