Any feedback?
Literature summary for 3.2.1.35 extracted from
- A hyaluronidase from Potamotrygon motoro (freshwater stingrays) venom: isolation and characterization (2008), Toxicon, 51, 1060-1067.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Potamotrygon motoro |  |
| Fe2+ | - |
Potamotrygon motoro | |
| heparin | - |
Potamotrygon motoro | |
| Mn2+ | - |
Potamotrygon motoro | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km (hyaluronan) (microgram/ml): 4.91, Vmax (hyaluronan): 2.02 U/min | Potamotrygon motoro |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79000 | - |
gel filtration | Potamotrygon motoro |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Potamotrygon motoro | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified by gel filtration on Sephacryl S-100 and ion-exchange chromatography on SP-Sepharose. Procedure provides 376.4fold purification with a 2.94% yield | Potamotrygon motoro |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronan + H2O | - |
Potamotrygon motoro | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 79000 Da, gel filtration and SDS-PAGE | Potamotrygon motoro |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hyaluronidase | - |
Potamotrygon motoro |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Potamotrygon motoro |
| 40 | - |
maximal activity | Potamotrygon motoro |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
enzyme is stable for at least 30 min when incubated at 20°C and 30°C | Potamotrygon motoro |
| 40 | - |
enzyme loses 70% of the activity at 40°C | Potamotrygon motoro |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
optimal pH | Potamotrygon motoro |
| 6 | - |
assay at | Potamotrygon motoro |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
