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1.15.1.1: superoxide dismutase

This is an abbreviated version!
For detailed information about superoxide dismutase, go to the full flat file.

Word Map on EC 1.15.1.1

Reaction

2 superoxide + 2 H+ =

O2
+
H2O2

Synonyms

AhSOD, alr2938, AmSOD, APE0743, ApMn-SOD1, ApMn-SOD2, ASAC_0498, Ca-Cu,Zn SOD, cambialistic superoxide dismutase, Cg-EcSOD, chloroplastic Fe-SOD, Cj-Cu, Zn SOD, cMn-SOD, cMnSOD, cold-active superoxide dismutase, copper, zinc superoxide dismutase, copper-zinc superoxide dismutase, copper/zinc superoxide dismutase, copper/zinc-superoxide dismutase, CpSOD, CSD1, CSD2, CtSOD, Cu, Zn SOD, Cu, Zn superoxide dismutase, Cu, Zn-superoxide dismutase, Cu, ZnSOD, Cu,Zn superoxide dismutase, Cu,Zn-SOD, Cu,Zn-superoxide dismutase, Cu,ZnSOD, Cu-Zn SOD, Cu-Zn superoxide dismutase, Cu-Zn-SOD, Cu/Zn SOD, Cu/Zn superoxide dismutase, Cu/Zn superoxide dismutase 1, Cu/Zn-SOD, Cu/Zn-SODI, Cu/Zn-SODII, Cu/Zn-superoxide dismutase, Cu/ZnSOD, cuprein, CuZn superoxide dismutase, CuZn superoxide dismutase 1, CuZn-SOD, CuZn-superoxide dismutase, CuZnSOD, cytMnSOD, cytocuprein, cytoplasmic manganese SOD, cytosolic Cu/Zn superoxide dismutase, cytosolic Cu/Zn-SOD, cytosolic manganese superoxide dismutase, DaSOD, dhsod-1, dismutase, superoxide, EC-SOD, ecCuZnSOD, ECSOD, ElFe-SOD, ElSOD, erythrocuprein, erythrocyte superoxide dismutase, eSOD, extracellular copper-zinc superoxide dismutase, extracellular CuZnSOD, extracellular SOD, extracellular superoxide dismutase, Fe-SOD, Fe-SODe, Fe-SOD_ASAC, Fe-superoxide dismutase, Fe-type SOD, Fe/Mn-SOD, Fe/Mn-type SOD, Fe/MnSOD, ferrisuperoxide dismutase, FeSOD, GTNG_2884, hEC-SOD, hemocuprein, hepatocuprein, high isoelectric point superoxide dismutase, intracellular Cu-Zn superoxide dismutase, iron SOD, iron SOD type, iron superoxide dismutase, iron-containing superoxide dismutase, iron-dependent superoxide dismutase, iron-superoxide dismutase, KmSod1p, LSOD, manganese superoxide dismutase, manganese-containing SOD, manganese-containing superoxide dismutase, MgMnSOD1, MgMnSOD2, mitMn-SOD, mitochondrial manganese superoxide dismutase, mMnSOD, Mn-containing superoxide dismutase, Mn-SOD, Mn-type SOD, Mn/Fe superoxide dismutase, MnSOD, MnSOD-2, MnSOD-3, MnSOD1, MnSOD47, More, mtMnSOD, mtSOD, nectarin I, neelaredoxin, Nlr, Of-cCu/ZnSOD, PASOD, perMn-SOD, pfSOD, PschSOD, PsSOD, PthipI-SODC, PthipI-SODC1, PthipI-SODC2, RmFeSOD, RsrSOD, SaCSD1, SaFe-SOD, sdB, SOD, SOD 1, SOD I, SOD-1, SOD-2, SOD-3, SOD-4, SOD1, SOD2, SOD3, SODA, SodB, SODB1, SODB2, SodC, SODF, SODI, SODII, SODIII, SODIV, SODS, SSO0316, superoxide dismutase, superoxide dismutase 1, superoxide dismutase I, superoxide dismutase II, superoxide dismutase [Cu-Zn]

ECTree

     1 Oxidoreductases
         1.15 Acting on superoxide as acceptor
             1.15.1 Acting on superoxide as acceptor (only sub-subclass identified to date)
                1.15.1.1 superoxide dismutase

Purification

Purification on EC 1.15.1.1 - superoxide dismutase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 forms, 1 dimer and 1 monomer
2 isoenzymes: superoxide dismutase I and II
-
3 electromorphs: AA, BB, AB
-
3 isoenzymes of anaero-SOD and 3 isoenzymes of aero-SOD
-
3 isoenzymes: 1, 2 and 3
-
5 isoenzymes
-
after overexpression in Escherichia coli
allozyme variants: DSDS and DSDF
-
analytical isolation of the enzyme in a 2D electrophoresis from strains KT2440 and PAO1, overview
-
both isozymes SODI, SODII
-
Cu, Zn-SOD and Mn-SOD
-
Cu,Zn-SOD
Cu,Zn-SOD from erythrocytes
-
Cu,Zn-SOD from liver
-
Cu,Zn-SOD from shoots and cotyledons
-
Cu,Zn-SOD wild-type and mutant recombinant from Escherichia coli
-
Cu,Zn-SOD, amino acid analysis
-
Cu,Zn-SOD, native and recombinant from Pichia pastoris
Cu,Zn-SOD, recombinant from Escherichia coli and native enzyme
-
Cu,Zn-SOD, wild-type and mutants recombinant from Spodoptera frugiperda cells
-
Cu/Zn-SOD from digestive gland and gills
-
Cu/Zn-SOD isozyme
DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant His6-and thioredoxin-tagged enzyme from Escherichia coli, 12.2fold by two steps of nickel affinity chromatography and desalting gel filtration
EC-SOD from aorta
-
EC-SOD recombinant from Escherichia coli as His-tagged protein and partially from insect cells
-
enzyme is stable under aerobic conditions
-
extracellular
-
extracellular enzyme
Fe-SOD
Fe-SOD and Mn-SOD
-
Fe-SOD and reconstructed Fe-/Mn-SOD
Fe-SOD, 2 isozymes
-
four isozymes by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration. SODI is purified 424fold, SODII 635fold, SODIII 336fold, and SODIV 489fold
-
from liver
-
from milk
-
intermediate between Fe- and Mn-SOD, contains Zn2+ as well
-
isoenzymes I and II
-
large scale immunoisolation of native mutant and wildtype SOD1
-
Mn-SOD
Mn-SOD from liver
Mn-SOD recombinant from Escherichia coli
native Cu,Zn-SOD 6.1fold from mycelial extracts
-
native Cu,Zn-SOD about 13fold by two steps of hydrophobic interaction chromatography and two steps of anion exchange chromatography
-
native Cu/Zn-SOD and Mn-SOD partially by subcellular fractionation, further purfication of mitochondrial Cu/Zn-SOD by anion exchange chromatography and, for analysis, by RP-HPLC
-
native EC-SOD from mouse lungs by hyaluronic acid affinity chromatography
-
native enzyme 115fold by heat treatment at 60°C for 10 min, gel filtration, anion exchange and hydrophobic interaction chromatography
native enzyme 28.5fold from strain NBIMCC 1984 by thermal treatment, dialysis, ion exchange chromatography, and chromatofocusing
-
native enzyme 308.5fold by ammonium sulfate precipitation, cation and anion ion exchange chromatography, and gel filtration
-
native enzyme 3fold from roots by ammonium sulfate fractionation and anion exchange chromatography
-
native enzyme 50fold from strain OS-77 by hydrophobic interaction and anion exchange chromatography, followed by gel filtration
-
native enzyme 61.5fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, hydrophobic interaction chromatography and again gel filtration, to homogeneity
-
native enzyme 7.49fold to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and hydrophobic interaction chromatography
Thermochaetoides thermophila
-
native enzyme by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration
-
native enzyme from bulbs by ammonium sulfate fractionation, dialysis, gel filtration, and anion exchange chromatography
-
native enzyme from hepatopancreas 6781fold by anion exchange chromatography and gel filtration
native enzyme from leaves to apparent homogeneity
-
native enzyme from mitochondrial intermembrane space of livber microsomes
-
native enzyme from plants 13.7fold by ammonium sulfate fractionation, cation and anion exchange chromatography, and gel filtration
-
native enzyme from post-ribosomal supernatant by anion exchange, hydrophobic interaction, and hydroxyapatite chromatography, followed by gel filtration, to homogeneity
-
native enzyme from sardinelle viscera to homogeneity 7.17fold by heat treatment at 65°C for 15 min, ammonium sulfate fractionation, and gel filtration
-
native enzyme from sperm 7.5-38fold, superoxide dismutase release from spermatozoa after cold shock and homogenization, followed by ion exchange chromatography and gel filtration
-
native enzyme from tubers/roots 3fold by ammonium sulfate fractionation and anion exchange chromatography
-
native enzyme to homogeneity
-
native enzyme to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography
native enzyme, and recombinant enzyme from Escherichia coli strain JM109(DE3)
native extracellular enzyme to homogeneity from the culture medium by ultrafiltration, gel filtration, dialysis, and anion exchange chromatography
-
native isozyme SODI by 23fold gel filtration, hydrophobic interaction chromatography, and anion-exchange chromatography
native isozyme SODII by 153fold gel filtration, hydrophobic interaction chromatography, and anion-exchange chromatography to homogeneity
native isozymes SODI and SODII 154fold and 98fold, respectively, from seedlings by heat treatment at 40°C for 15 min, ammonium sulfate fractionation, anionic exchange chromatography, and gel filtration
-
native peroxisomal Mn-SOD 5600fold from peroxisomal membranes, to homogeneity by ammonium sulfate fractionation, batch anion-exchange chromatography, and anion-exchange and gel filtration, mitochondrial Mn-SOD partially
nectarin I: Mn-SOD from nectar
-
overview: purification of extracellular superoxide dismutases
partially
purification of extracellular enzyme Fe-SOD 101.62fold from human blood serum by anion exchange chromatography and gel filtration
-
purification of extracellular enzyme Fe-SOD 103.73fold from human blood serum by anion exchange chromatography and gel filtration
-
recombinant chimera MnSOD-VHb from Escherichia coli strain BL21(DE3)
-
recombinant Cu, ZnSOD from Pichia pastoris strain GS115 by anion exchange chromatography
Thermochaetoides thermophila
recombinant Cu,Zn-SOD
-
recombinant Cu,ZnSOD from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, cleavage of the GST fusion protein
recombinant enzyme 3.4fold from Escherichia coli strain BL21 (DE3) by two-stage ultrafiltration to 92.6% purity
-
recombinant enzyme expressed in Escherichia coli
recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, to homogeneity
-
recombinant enzyme from Escherichia coli strain BL21(DE3)
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant enzyme from Escherichia coli strain BL21(DE3) to homogeneity
-
recombinant enzyme from Escherichia coli strain BL21(DE3)pLys to near homogeneity
recombinant enzyme from Escherichia coli strain QC779
recombinant enzyme from Pichia pastoris strain GS115 by anion exchange chromatography
recombinant enzyme from Pichia pastoris strain GS115 by dialysis and anion exchange chromatography
Thermochaetoides thermophila
recombinant enzyme with his-tag
recombinant from Escherichia coli
-
recombinant GST-tagged SeCuZnSOD from Escherichia coli by glutathione affinity chromatography
recombinant His-tagged Cu,Zn-SOD from Escherichia coli
recombinant His-tagged enzyme 12.6fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme 14fold from Escherichia coli strain strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme 25fold from Escherichia coli strain BL21(DE3) by immobilized metal-ion affinity and ion exchange chromatography, and isoelectric focusing to over 95% purity
recombinant His-tagged enzyme 3.2fold from Escherichia coli strain Rosetta-gami by nickel affinity chromatography
recombinant His-tagged enzyme by nickel affinity chromatography from Escherichia coli strain Rosetta-gami(DE3)
recombinant His-tagged enzyme from Escherichia coli
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and dialysis, anion exchange chromatography and dialysis, and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to near homogeneity
recombinant His-tagged enzyme from Escherichia coli strain BL21-SI by nickel affinity chromatography, dialysis, and ultrafiltration to over 97% purity
recombinant His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography, dialysis, and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain Rosetta-gami by nickel affinity chromatgraphy
-
recombinant His-tagged enzyme from Escherichia coli strain Rosetta-gami by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strains DH5alpha and M15 by nickel affinity chromatography
recombinant His-tagged MnSOD-2 and MnSOD-3 by nickel affinity and anion exchange chromatography, followed by gel filtration
-
recombinant His-tagged SOD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged soluble chloroplastic isoform CuZn-SOD from Escherichia coli by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes and His-tagged SOD1-Lys7 from Escherichia coli BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type enzyme SODAp and NTD-fused N-terminal domain ntdSODAp from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His6-tagged type A isozyme from Escherichia coli strain BL21(DE3) by His-trap affinity chromatography and gel filtration
-
recombinant MBP-tagged enzyme from Escherichia coli strain BL21(DE3) by amylose affinity chromatography
recombinant Mn-SOD from Escherichia coli strain QC774 by anion exchange chromatography
-
recombinant MnSOD from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, cleavage of the GST fusion protein
recombinant SOD1 from Leishmania tarentolae strain P10 to 90% purity by ultracentrifugation, hydrophobic interaction chromatography and dialysis
recombinant soluble Cp-icCuZnSOD by nickel affinity chromatography
-
recombinant soluble enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant wild-type and mutant from Escherichia coli
recombinant wild-type and truncated mutant FLAG-tagged hEC-SOD enzymes from Spodoptera frugiperda Sf9 cells by affinity chromatography
SOD-I
-
SOD-I and SOD-III, of 4 isoenzymes
-
SOD-II, SOD-III, SOD-IV
-
soluble recombinant enzyme from Escherichia coli by ammonium sulfate fractionation and anion exchange chromatography to homogeneity
soluble recombinant enzyme SOD2 from Escherichia coli cell-free extract by dialysis, anion exchange chromatography, again dialysis, and ultrafiltration
structural intermediate between Mn-SOD and Fe-SOD
-
var. gemmifera, 3 isoenzymes
-