Application | Comment | Organism |
---|---|---|
diagnostics | the enzyme might be used to develop a biosensor for the detection of antioxidants and free radical activity | Bacillus sp. (in: Bacteria) |
Cloned (Comment) | Organism |
---|---|
gene sodA, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by hydrogen peroxide or potassium cyanide | Bacillus sp. (in: Bacteria) | |
Sodium azide | - |
Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | a Mn-superoxide dismutase, conserved manganese-binding site residues are H28, H83, D165, and H169 | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22650 | - |
x * 22650, sequence calculation | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 O2.- + 2 H+ | Bacillus sp. (in: Bacteria) | - |
O2 + H2O2 | - |
? | |
2 O2.- + 2 H+ | Bacillus sp. (in: Bacteria) MHS47 | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | E9LK91 | collected from soil and water samples from hot springs in Mae Hong Son Province of Thailand, gene sodA | - |
Bacillus sp. (in: Bacteria) MHS47 | E9LK91 | collected from soil and water samples from hot springs in Mae Hong Son Province of Thailand, gene sodA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 14fold from Escherichia coli strain strain BL21(DE3) by nickel affinity chromatography | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3538 | - |
pH 7.5, 37°C, recombinant enzyme | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 O2.- + 2 H+ | - |
Bacillus sp. (in: Bacteria) | O2 + H2O2 | - |
? | |
2 O2.- + 2 H+ | - |
Bacillus sp. (in: Bacteria) MHS47 | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22650, sequence calculation | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
MnSOD47 | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 80 | activity range, profile overview | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
purified recombinant His-tagged enzyme, 40 min, 50% activity remaining | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | activity range, profile overview | Bacillus sp. (in: Bacteria) |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus sp. (in: Bacteria) | sequence calculation | - |
5.31 |
General Information | Comment | Organism |
---|---|---|
additional information | MnSOD47 contains the decapod crustacean signature (DXWEHXXY), which is a specific characteristic of Mn-superoxide dismutase | Bacillus sp. (in: Bacteria) |
physiological function | superoxide dismutases are considered key enzymes in the control of oxidative stress because they can protect oxygen-metabolizing cells against the harmful effects of superoxide free radicals | Bacillus sp. (in: Bacteria) |