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Literature summary for 1.15.1.1 extracted from

  • Reinards, R.; Altdorf, R.; Ohlenbusch, H.D.
    Purification and properties of a manganese-containing superoxide dismutase from Acholeplasma laidlawii (1984), Hoppe-Seyler's Z. Physiol. Chem., 365, 577-585.
    View publication on PubMed
Search for more literature for 1.15.1.1

General Stability

General Stability Organism
lyophilization: less than 10% loss of activity Acholeplasma laidlawii

Inhibitors

Inhibitors Comment Organism Structure
H2O2 no inhibition: Mn-SOD Acholeplasma laidlawii

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.5 mol per mol of subunit Acholeplasma laidlawii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21600
-
 2 * 21600, SDS-PAGE after denaturation in boiling SDS Acholeplasma laidlawii
41500
-
 gel filtration Acholeplasma laidlawii
41500
-
 Mn-SOD Acholeplasma laidlawii

Organism

Organism UniProt Comment Textmining
Acholeplasma laidlawii
-
 Mn-SOD
-
Acholeplasma laidlawii Mn-SOD
-
 Mn-SOD
-

Purification (Commentary)

Purification (Comment) Organism
Mn-SOD Acholeplasma laidlawii

Reaction

Reaction Comment Organism Reaction ID
2 superoxide + 2 H+ = O2 + H2O2 amino acid composition, comparison Acholeplasma laidlawii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Acholeplasma laidlawii

Storage Stability

Storage Stability Organism
4°C, 10 mM phosphate buffer, pH 7.2, 4 weeks Acholeplasma laidlawii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O2- + H+
-
 Acholeplasma laidlawii O2 + H2O2
-
 ?
O2- + H+
-
 Acholeplasma laidlawii Mn-SOD O2 + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 21600, SDS-PAGE after denaturation in boiling SDS Acholeplasma laidlawii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 1 h, about 20% loss of activity Acholeplasma laidlawii
50
-
 1 h, about 55% loss of activity Acholeplasma laidlawii
55
-
 10 min, about 45% loss of activity, 30 min, 75% loss of activity Acholeplasma laidlawii
60
-
 10 min, about 75% loss of activity Acholeplasma laidlawii