Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and anaylsis, expression of wild-type and mutant in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
Y88F | site-directed mutagenesis, substitution of Tyr88 to Phe does not affect the metal specificity of the enzyme | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fluoride | recombinant Fe-reconstituted SOD | Saccharolobus solfataricus | |
H2O2 | distinguishes Fe-SOD from Mn-SOD, since it inactivates only Fe-SOD | Saccharolobus solfataricus | |
additional information | recombinant Fe-reconstituted SOD is not inhibited by azide, steric hindrance in the substrate funnel of the enzyme prevents the access of N3- but allows O2- and F- access to the active site | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe/Mn-type SOD | Saccharolobus solfataricus | |
Mn2+ | Fe/Mn-type SOD | Saccharolobus solfataricus | |
additional information | metal content analysis of the recombinant enzyme | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | P80857 | DSM 1616 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant from Escherichia coli | Saccharolobus solfataricus |
Renatured (Comment) | Organism |
---|---|
reconstitution of Fe-SOD from purified recombinant apo-enzyme, apo-enzyme preparation: purified recombinant enzyme is denatured in buffer containing 50 mM acetate buffer, pH 3.8, 6 M guanidine hydrochloride, and 10 mM EDTA for 16 h at 50°C, followed by dialysis against the same buffer containing MnSO4 instead of EDTA, and removal of guanidine hydrochloride in a second dialysis step, followed by gel filtration, overview | Saccharolobus solfataricus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Sulfolobus solfataricus grows between 50°C and 87°C with an optimum growing temperature of 87°C, but it neither grows nor survives at 90°C | Saccharolobus solfataricus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
reconstituted Mn-SOD | Saccharolobus solfataricus |
4.3 | - |
reconstituted Mn-SOD mutant Y88F | Saccharolobus solfataricus |
481 | - |
reconstituted Fe-SOD | Saccharolobus solfataricus |
755 | - |
reconstituted Fe-SOD mutant Y88F | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
SOD | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
2 h, purified recombinant enzyme, 96% remaining activity, half-life is 33 h, inactivation according to first order kinetics | Saccharolobus solfataricus |
100 | - |
purified recombinant enzyme, half-life is 8.7 h, inactivation according to first order kinetics | Saccharolobus solfataricus |
105 | - |
purified recombinant enzyme, half-life is 1.5 h, inactivation according to first order kinetics | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Saccharolobus solfataricus |