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Literature summary for 1.15.1.1 extracted from
- Isolation and characterization of cytosolic and chloroplast isoenzymes of Cu,Zn-superoxide dismutase from tomato leaves and their relationship to other Cu,Zn-superoxide dismutases (1986), Biochim. Biophys. Acta, 874, 99-115.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| urea: 8 M, 0°C, isoenzyme I unfolds immediately, isoenzyme II stays folded | Solanum lycopersicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | Cu,Zn-SOD | Solanum lycopersicum |  |
| N3- | - |
Solanum lycopersicum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Solanum lycopersicum | 9507 | - |
| cytoplasm | - |
Solanum lycopersicum | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1.63-1.78 mol per mol of isoenzyme I | Solanum lycopersicum | |
| Cu2+ | 1.86-1.97 mol per mol of isoenzyme II | Solanum lycopersicum | |
| Zn2+ | 1.34-1.81 mol per mol of isoenzyme I | Solanum lycopersicum | |
| Zn2+ | 1.9-20.0 mol per mol of isoenzyme II | Solanum lycopersicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15100 | - |
2 * 15100, isoenzyme II, SDS-PAGE | Solanum lycopersicum |
| 30000 | - |
Cu,Zn-SOD isoenzyme I, gel filtration, sedimentation equilibrium centrifugation | Solanum lycopersicum |
| 33000 | - |
Cu,Zn-SOD isoenzyme II, gel filtration, sedimentation equilibrium analysis | Solanum lycopersicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | - |
Cu,Zn-SOD: 2 isozymes I and II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzymes I and II | Solanum lycopersicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Solanum lycopersicum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Solanum lycopersicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O2- + H+ | - |
Solanum lycopersicum | O2 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 15100, isoenzyme II, SDS-PAGE | Solanum lycopersicum |
| dimer | isoenzyme I, SDS-PAGE | Solanum lycopersicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 7.8, half-life: 70 min, isoenzyme I, 177 min, isoenzyme II | Solanum lycopersicum |
| 60 | - |
pH 7. 8, half-life: 5 min, isoenzyme I, 34 min, isoenzyme II | Solanum lycopersicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
isoenzyme I | Solanum lycopersicum |
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