Cloned (Comment) | Organism |
---|---|
gene cz1, expression of Cu,ZnSOD in Pichia pastoris strain GS115 | Thermochaetoides thermophila |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging-drop vapour-diffusion, 0.0025 ml of 10.5 mg/ml protein in 20 mM Tris-HCl, pH 8.2, are mixed with 0.0025 ml of reservoir solution containing 1.4 M sodium potassium phosphate, pH 8.2, equilibration against 0.8 ml of reservoir solution, 16°C, 4 days, method screening and optimization, X-ray diffraction structure determination and analysis at 1.9 A resolution | Thermochaetoides thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a CuZn-superoxide dismutase | Thermochaetoides thermophila | |
Zn2+ | a CuZn-superoxide dismutase | Thermochaetoides thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17700 | - |
x * 17700, recombinant enzyme, SDS-PAGE | Thermochaetoides thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermochaetoides thermophila | Q1HEQ0 | gene cz1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Pichia pastoris strain GS115 by dialysis and anion exchange chromatography | Thermochaetoides thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
127.4 | - |
purified recombinant enzyme, pH 6.5, 60°C | Thermochaetoides thermophila |
Subunits | Comment | Organism |
---|---|---|
? | x * 17700, recombinant enzyme, SDS-PAGE | Thermochaetoides thermophila |
Synonyms | Comment | Organism |
---|---|---|
Cu,Zn superoxide dismutase | - |
Thermochaetoides thermophila |
Cu,ZnSOD | - |
Thermochaetoides thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant enzyme | Thermochaetoides thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the puurified recombinant enzyme retains 93% of maximal activity after 60 min | Thermochaetoides thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
recombinant enzyme | Thermochaetoides thermophila |