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Literature summary for 1.15.1.1 extracted from

  • Wakadkar, S.; Zhang, L.Q.; Li, D.C.; Haikarainen, T.; Dhavala, P.; Papageorgiou, A.C.
    Expression, purification and crystallization of Chaetomium thermophilum Cu,Zn superoxide dismutase (2010), Acta Crystallogr. Sect. F, 66, 1089-1092.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene cz1, expression of Cu,ZnSOD in Pichia pastoris strain GS115 Thermochaetoides thermophila

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging-drop vapour-diffusion, 0.0025 ml of 10.5 mg/ml protein in 20 mM Tris-HCl, pH 8.2, are mixed with 0.0025 ml of reservoir solution containing 1.4 M sodium potassium phosphate, pH 8.2, equilibration against 0.8 ml of reservoir solution, 16°C, 4 days, method screening and optimization, X-ray diffraction structure determination and analysis at 1.9 A resolution Thermochaetoides thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a CuZn-superoxide dismutase Thermochaetoides thermophila
Zn2+ a CuZn-superoxide dismutase Thermochaetoides thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17700
-
x * 17700, recombinant enzyme, SDS-PAGE Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila Q1HEQ0 gene cz1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Pichia pastoris strain GS115 by dialysis and anion exchange chromatography Thermochaetoides thermophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
127.4
-
purified recombinant enzyme, pH 6.5, 60°C Thermochaetoides thermophila

Subunits

Subunits Comment Organism
? x * 17700, recombinant enzyme, SDS-PAGE Thermochaetoides thermophila

Synonyms

Synonyms Comment Organism
Cu,Zn superoxide dismutase
-
Thermochaetoides thermophila
Cu,ZnSOD
-
Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
recombinant enzyme Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
the puurified recombinant enzyme retains 93% of maximal activity after 60 min Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
recombinant enzyme Thermochaetoides thermophila