Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudoalteromonas sp. ANT506 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chloroform | recombinant PsSOD is sensitive to a mixture of chloroform and ethanol in the ratio 3:5. Mixing the recombinant PsSOD enzyme and the solvent mixture by 2:1 and 1:1, the relative activity is 36.4 and 13.6%, respectively. In addition, no enzymatic activity remains after incubation at 1:2 mixture | Pseudoalteromonas sp. ANT506 | |
ethanol | recombinant PsSOD is sensitive to a mixture of chloroform and ethanol in the ratio 3:5. Mixing the recombinant PsSOD enzyme and the solvent mixture by 2:1 and 1:1, the relative activity is 36.4 and 13.6%, respectively. In addition, no enzymatic activity remains after incubation at 1:2 mixture | Pseudoalteromonas sp. ANT506 | |
Fe3+ | 9.7% inhibition | Pseudoalteromonas sp. ANT506 | |
H2O2 | preincubation in 5 mM H2O2 for 30 min results in a loss of 91% activity | Pseudoalteromonas sp. ANT506 | |
NaCl | 60% activity remains at 2.5 M NaCl | Pseudoalteromonas sp. ANT506 | |
Ni2+ | 33.7% inhibition | Pseudoalteromonas sp. ANT506 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Pseudoalteromonas sp. ANT506 | |
Cu2+ | activates | Pseudoalteromonas sp. ANT506 | |
Fe2+ | a Fe/Mn-SOD | Pseudoalteromonas sp. ANT506 | |
Mg2+ | activates | Pseudoalteromonas sp. ANT506 | |
Mn2+ | a Fe/Mn-SOD | Pseudoalteromonas sp. ANT506 | |
Zn2+ | activates | Pseudoalteromonas sp. ANT506 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | Pseudoalteromonas sp. ANT506 | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. ANT506 | A0A0S2E8T0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 12.6fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudoalteromonas sp. ANT506 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | - |
Pseudoalteromonas sp. ANT506 | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 21400, about, sequence calculation, 1 * 27000, recombinant His-tagged enzyme, SDS-PAGE | Pseudoalteromonas sp. ANT506 |
Synonyms | Comment | Organism |
---|---|---|
Fe/Mn-SOD | - |
Pseudoalteromonas sp. ANT506 |
PsSOD | - |
Pseudoalteromonas sp. ANT506 |
SOD | - |
Pseudoalteromonas sp. ANT506 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant His-tagged enzyme | Pseudoalteromonas sp. ANT506 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
50 | activity range, 13.9% of maximal activity at 0°C, 25.7% at 10°C | Pseudoalteromonas sp. ANT506 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant His-tagged enzyme, 40 min, 82.2% activity remains | Pseudoalteromonas sp. ANT506 |
50 | - |
purified recombinant His-tagged enzyme, 40 min, 59.4% activity remains | Pseudoalteromonas sp. ANT506 |
70 | - |
purified recombinant His-tagged enzyme, 20 min, almost no activity remains | Pseudoalteromonas sp. ANT506 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant His-tagged enzyme | Pseudoalteromonas sp. ANT506 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | over 90% of maximal enzyme activity at pH 7.0-9.0, rapid loss of activity below pH 5.0 and above pH 10.0 | Pseudoalteromonas sp. ANT506 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudoalteromonas sp. ANT506 | sequence calculation | - |
5.09 |