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Literature summary for 1.15.1.1 extracted from
- A superoxide dismutase from the archaeon Sulfolobus solfataricus is an extracellular enzyme and prevents the deactivation by superoxide of cell-bound proteins (2000), Eur. J. Biochem., 267, 235-243.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | extracellular Fe-SOD, associated with cell-surface | Saccharolobus solfataricus | 5618 | - |
| extracellular | - |
Saccharolobus solfataricus | - |
- |
| extracellular | Fe-SOD in culture fluid | Saccharolobus solfataricus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24140 | - |
x * 24140, mass spectrometry | Saccharolobus solfataricus |
| 63000 | - |
gel filtration | Saccharolobus solfataricus |
| 63000 | - |
Fe-SOD, gel filtration | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 O2.- + 2 H+ | Saccharolobus solfataricus | - |
O2 + H2O2 | - |
? |  |
| 2 O2.- + 2 H+ | Saccharolobus solfataricus Gtheta | - |
O2 + H2O2 | - |
? | |
| O2- + H+ | Saccharolobus solfataricus | defense against oxidants | O2 + H2O2 | - |
? | |
| O2- + H+ | Saccharolobus solfataricus Gtheta | defense against oxidants | O2 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
| Saccharolobus solfataricus | - |
oxygen-inducible, extracellular Fe-SOD | - |
| Saccharolobus solfataricus Gtheta | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Fe-SOD | Saccharolobus solfataricus |
| native extracellular enzyme to homogeneity from the culture medium by ultrafiltration, gel filtration, dialysis, and anion exchange chromatography | Saccharolobus solfataricus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharolobus solfataricus |
| 17.3 | - |
purified extracellular enzyme, pyrogallol autoxidation inhibition assay method | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 O2.- + 2 H+ | - |
Saccharolobus solfataricus | O2 + H2O2 | - |
? | |
| 2 O2.- + 2 H+ | - |
Saccharolobus solfataricus Gtheta | O2 + H2O2 | - |
? | |
| O2- + H+ | - |
Saccharolobus solfataricus | O2 + H2O2 | - |
? | |
| O2- + H+ | defense against oxidants | Saccharolobus solfataricus | O2 + H2O2 | - |
? | |
| O2- + H+ | - |
Saccharolobus solfataricus Gtheta | O2 + H2O2 | - |
? | |
| O2- + H+ | defense against oxidants | Saccharolobus solfataricus Gtheta | O2 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 22500-24000, Fe-SOD, SDS-PAGE | Saccharolobus solfataricus |
| trimer or tetramer | x * 24140, mass spectrometry | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SOD | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
assay at | Saccharolobus solfataricus |
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