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Literature summary for 1.15.1.1 extracted from
- Characterization of a new Cu/Zn-superoxide dismutase from Beauveria bassiana and two site-directed mutations crucial to its antioxidation activity without chaperon (2010), Enzyme Microb. Technol., 46, 217-222.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene BbSod1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, expression as His-tagged wild-type and mutant enzymes and as His-tagged Saccharomyces cerevisiae superoxide dismutase 1 copper chaperone-fusion enzyme, i.e. SOD1-Lys7, in Escherichia coli BL21(DE3) | Beauveria bassiana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a chimeric SOD1 mutant fused to Saccharomyces cerevisiae superoxide dismutase 1 copper chaperone-fusion enzyme, Lys7, the chimeric mutant SOD1-Lys7 shows increased activirty compared to the wild-type SOD1 and SOD1 mutant P143S/P145L | Beauveria bassiana |
| P143S/P145L | site-directed mutagenesis, gain-of-function, the mutant shows increased activirty compared to the wild-type SOD1 | Beauveria bassiana |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a CuZn-superoxide dismutase | Beauveria bassiana |  |
| Zn2+ | a CuZn-superoxide dismutase | Beauveria bassiana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x *17000, recombinant SOD1, SDS-PAGE, x * 45000, recombinant SOD1-Lys7, SDS-PAGE | Beauveria bassiana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Beauveria bassiana | C7EXL1 | gene BbSod1 | - |
| Beauveria bassiana 2860 | C7EXL1 | gene BbSod1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes and His-tagged SOD1-Lys7 from Escherichia coli BL21(DE3) by nickel affinity chromatography | Beauveria bassiana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 178.7 | - |
recombinant wild-type SOD1, pH not specified in the publication, temperature not specified in the publication | Beauveria bassiana |
| 1331 | - |
recombinant SOD1-Lys7 chimera, pH not specified in the publication, temperature not specified in the publication | Beauveria bassiana |
| 3788 | - |
recombinant SOD1 mutant P143S/P145L, pH not specified in the publication, temperature not specified in the publication | Beauveria bassiana |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x *17000, recombinant SOD1, SDS-PAGE, x * 45000, recombinant SOD1-Lys7, SDS-PAGE | Beauveria bassiana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cu/Zn-superoxide dismutase | - |
Beauveria bassiana |
| SOD1 | - |
Beauveria bassiana |
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Release 2023.1 (January 2023)
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