1.1.1.50: 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific)

This is an abbreviated version, for detailed information about 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific), go to the full flat file.

Reaction

a 3alpha-hydroxysteroid
+
NAD(P)+
=
a 3-oxosteroid
+
NAD(P)H
+
H+

Synonyms

3 alpha-hydroxysteroid dehydrogenase, 3 alphaHD, 3-alpha-HSD, 3alpha-HSD, 3alpha-HSD/CR, 3alpha-HSOR, 3alpha-hydroxysteroid dehydrogenase, 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase, 3alpha-hydroxysteroid oxido-reductase, 3alpha-hydroxysteroid oxidoreductase, 3alpha-hydroxysteroid-5beta-oxidoreductase activity, 3alpha-OR, 3alpha-oxidoreductase, 3alpha/3beta-hydroxysteroid dehydrogenase, 3alphaHSD, 3HSD, 5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase, AKR1C, AKR1C1-4, AKR1C17, AKR1C2, AKR1C3, AKR1C4, AKR1C9, aldo-keto reductase 1C2, bile-acid binding protein, chlordecone reductase, DD2, DD21, dihydrodiol dehydrogenase, HSD, HSD28, HSD29, hsdA, HSDH, hydroxyprostaglandin dehydrogenase, More, NAD(P)+-3alpha-hydroxysteroid dehydrogenase, NAD+-dependent 3alpha-HSD, NADP(H)-dependent 3alpha-HSD, NADPH:5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase, prostaglandin F2alpha-synthase, PT3HSD, sterognost 3alpha dehydrogenase, 3alpha-hydroxy steroid, type 2 3alpha-hydroxysteroid dehydrogenase/type 5 17beta-hydroxysteroid dehydrogenase, type 3 3alpha-hydroxysteroid dehydrogenase, type 5 17beta-hydroxysteroid dehydrogenase, type I 3alpha-HSD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.50 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific)

Specific Activity

Specific Activity on EC 1.1.1.50 - 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000023
-
3alpha-hydroxysteroid dehydrogenase activity in the liver
0.00000027
-
3alpha-hydroxysteroid dehydrogenase activity in the proximal intestine
0.00000032
-
3alpha-hydroxysteroid dehydrogenase activity in the distal intestine
0.00002
-
purified recombinant isozymes AKR1C1 and AKR1C3, substrate 3alpha-hydroxytibolone
0.00015
-
purified recombinant isozyme AKR1C3, substrate 3beta-hydroxytibolone
0.00024
-
purified enzyme, substrate 5beta-androstan-3,17-dione
0.0005
-
substrate androsterone with cofactor NADP+, detection radiometrically
0.00079
-
purified recombinant isozyme AKR1C1, substrate 3beta-hydroxytibolone
0.0013
-
substrate 5alpha-dihydrotestosterone with cofactor NADP+, detection radiometrically
0.0025
-
substrate 5alpha-dihydrotestosterone with cofactor NADPH, detection spectrophotometrically
0.0037
-
purified recombinant isozyme AKR1C3, substrate 4-pregnen-3alpha,20beta-diol
0.0039
-
purified recombinant isozyme AKR1C1, substrate 4-pregnen-3alpha,20beta-diol
0.0042
-
substrate 5alpha-dihydrotestosterone with cofactor NADPH, detection radiometrically
0.0048
-
substrate 3alpha-androstanediol with cofactor NADP+, detection spectrophotometrically
0.00653
-
purified recombinant isozyme AKR1C4, substrate 3beta-hydroxytibolone
0.0085
-
substrate 3alpha-androstanediol with cofactor NADP+, detection radiometrically
0.011
-
-
0.0111
-
substrate androstanedione with cofactor NADPH, detection spectrophotometrically
0.0134
-
substrate 3alpha-androstanediol with cofactor NADP+, detection fluorometrically
0.0135
-
-
0.0188
-
purified recombinant isozyme AKR1C4, substrate 3alpha-hydroxytibolone
0.0253
-
purified recombinant isozyme AKR1C2, substrate 3beta-hydroxytibolone
0.031
-
substrate dihydrotestosterone
0.04
-
5alpha-pregnan-3,20-dione
0.07
-
recombinant AKR1C17 in cell extract, substrate 4-androsten-3alpha-ol-17-one
0.119
-
purified recombinant isozyme AKR1C4, substrate 4-pregnen-3alpha,20beta-diol
0.13
-
substrate 4-nitrobenzaldehyde with cofactor NADPH, detection spectrophotometrically
0.15
-
purified enzyme
0.23
-
purified recombinant AKR1C17, substrate 4-androsten-3alpha-ol-17-one
0.323
-
3alpha-hydroxy-5alpha-pregnan-20-one
0.538
-
substrate 9,10-phenanthrenequinone with cofactor NADPH, detection spectrophotometrically
1.56
-
substrate 5beta-dihydroprogesterone, young leaves
2.5
-
fusidic acid, oxidation
7
-
commercially purified enzyme in Tris-HCl/co-solvent 1-butyl-3-methylimidazolium L-lactate at pH 7.6 and 25°C
23.6
-
purified enzyme, substrate cholate with NAD+
27.4
-
cholic acid, oxidation
64.5
-
5alpha-dihydrotestosterone, reduction
76.1
-
purified recombinant isozyme AKR1C2, substrate 4-pregnen-3alpha,20beta-diol
85.7
-
androstanedione, reduction
119
-
androsteone, oxidation
869.8
-
substrate 5beta-dihydroprogesterone, green shoot culture
2999 - 4199
-
green leaves
additional information