Cloned (Comment) | Organism |
---|---|
overexpression as His-tagged fusion protein in Escherichia coli | Comamonas testosteroni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0061 | - |
Fusidic acid | - |
Comamonas testosteroni | |
0.0184 | - |
cholic acid | - |
Comamonas testosteroni | |
0.0223 | - |
5alpha-dihydrotestosterone | - |
Comamonas testosteroni | |
0.0311 | - |
androsterone | - |
Comamonas testosteroni | |
0.0422 | - |
androstanedione | - |
Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26400 | - |
2 * 26400, SDS-PAGE and sequence analysis | Comamonas testosteroni |
49400 | - |
gel filtration | Comamonas testosteroni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant, His-tagged from Escherichia coli | Comamonas testosteroni |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.5 | - |
fusidic acid, oxidation | Comamonas testosteroni |
27.4 | - |
cholic acid, oxidation | Comamonas testosteroni |
64.5 | - |
5alpha-dihydrotestosterone, reduction | Comamonas testosteroni |
85.7 | - |
androstanedione, reduction | Comamonas testosteroni |
119 | - |
androsteone, oxidation | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydrotestosterone + NAD(P)H + H+ | - |
Comamonas testosteroni | 5alpha-androstan-3alpha,17beta-diol + NAD(P)+ | - |
r | |
5alpha-dihydrotestosterone + NAD(P)H + H+ | - |
Comamonas testosteroni | (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+ | - |
r | |
5beta-androstan-3,17-dione + NAD(P)H | - |
Comamonas testosteroni | 5beta-androstan-17one-3-ol + NAD(P)+ | - |
r | |
androsterone + NAD(P)+ | - |
Comamonas testosteroni | 5alpha-androstan-3,17-dione + NAD(P)H | - |
r | |
androsterone + NAD+ | - |
Comamonas testosteroni | androstanedione + NADH | - |
? | |
cholic acid + NAD(P)+ | - |
Comamonas testosteroni | 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
r | |
fusidic acid + NAD+ | - |
Comamonas testosteroni | ? | - |
r | |
metapyrone + NADH | - |
Comamonas testosteroni | ? | - |
? | |
p-nitrobenzaldehyde + NAD(P)H | - |
Comamonas testosteroni | p-nitrobenzylalcohol + NAD(P)+ | - |
r | |
p-nitrobenzylalcohol + NAD+ | - |
Comamonas testosteroni | p-nitrobenzaldehyde + NADH | - |
r | |
progesterone + NADH | - |
Comamonas testosteroni | ? | - |
r | |
testosterone + NAD(P)+ | - |
Comamonas testosteroni | androstenedione + NAD(P)H + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26400, SDS-PAGE and sequence analysis | Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Comamonas testosteroni |