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show all sequences of 1.1.1.50

Mechanistic roles of Ser-114, Tyr-155, and Lys-159 in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni

Hwang, C.; Chang, Y.; Hsu, C.; Hsu, H.; Li, C.; Pon, H.; J. Biol. Chem. 280, 3522-3528 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Comamonas testosteroni
Engineering
Amino acid exchange
Commentary
Organism
K159A
site-directed mutagenesis, the mutant shows altered kinetics and pH profile, and 200fold reduced activity compared to the wild-type
Comamonas testosteroni
S114A
site-directed mutagenesis, the mutant shows altered kinetics and pH profile, and 3400fold reduced activity compared to the wild-type
Comamonas testosteroni
S114A/Y155F
site-directed mutagenesis, the mutant shows altered kinetics and pH profile, and 200000fold reduced activity compared to the wild-type
Comamonas testosteroni
Y155F
site-directed mutagenesis, the mutant shows altered kinetics and pH profile, and 2800fold reduced activity compared to the wild-type
Comamonas testosteroni
Y155F/K159A
site-directed mutagenesis, the mutant shows altered kinetics and pH profile, and 9400fold reduced activity compared to the wild-type
Comamonas testosteroni
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetics
Comamonas testosteroni
0.00044
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant S114A/Y155F
Comamonas testosteroni
0.0055
-
androsterone
pH 9.0, 25C, recombinant His-tagged wild-type
Comamonas testosteroni
0.007
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant S114A
Comamonas testosteroni
0.065
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant K159A
Comamonas testosteroni
0.074
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant Y155F
Comamonas testosteroni
0.1
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant Y155F/K159A
Comamonas testosteroni
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
about, recombinant His-tagged wild-type and mutant enzymes, gel filtration
Comamonas testosteroni
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
androsterone + NAD+
Comamonas testosteroni
-
5alpha-androstane-3,17-dione + NADH
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Comamonas testosteroni
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, and gel filtration to homogeneity
Comamonas testosteroni
Reaction
Reaction
Commentary
Organism
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+
the catalytic triad is composed of Ser-114, Tyr-155, and Lys-159, structural analysis and sequence alignment of the short chain dehydrogenase/reductase family
Comamonas testosteroni
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Comamonas testosteroni
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
androsterone + NAD+
-
669389
Comamonas testosteroni
5alpha-androstane-3,17-dione + NADH
-
-
-
r
additional information
bifunctional enzyme also catalyzing the reaction of carbonyl reductase, EC 1.1.1.184
669389
Comamonas testosteroni
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 28349-28425, recombinant His-tagged wild-type and mutant enzymes, mass spectrometry
Comamonas testosteroni
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Comamonas testosteroni
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0028
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant S114A/Y155F
Comamonas testosteroni
0.003
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant Y155F/K159A
Comamonas testosteroni
0.12
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant S114A
Comamonas testosteroni
1.5
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant Y155F
Comamonas testosteroni
18.9
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant K159A
Comamonas testosteroni
318
-
androsterone
pH 9.0, 25C, recombinant His-tagged wild-type
Comamonas testosteroni
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Comamonas testosteroni
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
11
pH profile, recombinant His-tagged wild-type and mutant enzymes
Comamonas testosteroni
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Comamonas testosteroni
NADH
-
Comamonas testosteroni
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Comamonas testosteroni
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Comamonas testosteroni
NADH
-
Comamonas testosteroni
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
K159A
site-directed mutagenesis, the mutant shows altered kinetics and pH profile, and 200fold reduced activity compared to the wild-type
Comamonas testosteroni
S114A
site-directed mutagenesis, the mutant shows altered kinetics and pH profile, and 3400fold reduced activity compared to the wild-type
Comamonas testosteroni
S114A/Y155F
site-directed mutagenesis, the mutant shows altered kinetics and pH profile, and 200000fold reduced activity compared to the wild-type
Comamonas testosteroni
Y155F
site-directed mutagenesis, the mutant shows altered kinetics and pH profile, and 2800fold reduced activity compared to the wild-type
Comamonas testosteroni
Y155F/K159A
site-directed mutagenesis, the mutant shows altered kinetics and pH profile, and 9400fold reduced activity compared to the wild-type
Comamonas testosteroni
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetics
Comamonas testosteroni
0.00044
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant S114A/Y155F
Comamonas testosteroni
0.0055
-
androsterone
pH 9.0, 25C, recombinant His-tagged wild-type
Comamonas testosteroni
0.007
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant S114A
Comamonas testosteroni
0.065
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant K159A
Comamonas testosteroni
0.074
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant Y155F
Comamonas testosteroni
0.1
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant Y155F/K159A
Comamonas testosteroni
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
-
about, recombinant His-tagged wild-type and mutant enzymes, gel filtration
Comamonas testosteroni
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
androsterone + NAD+
Comamonas testosteroni
-
5alpha-androstane-3,17-dione + NADH
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, and gel filtration to homogeneity
Comamonas testosteroni
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Comamonas testosteroni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
androsterone + NAD+
-
669389
Comamonas testosteroni
5alpha-androstane-3,17-dione + NADH
-
-
-
r
additional information
bifunctional enzyme also catalyzing the reaction of carbonyl reductase, EC 1.1.1.184
669389
Comamonas testosteroni
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 28349-28425, recombinant His-tagged wild-type and mutant enzymes, mass spectrometry
Comamonas testosteroni
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Comamonas testosteroni
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0028
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant S114A/Y155F
Comamonas testosteroni
0.003
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant Y155F/K159A
Comamonas testosteroni
0.12
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant S114A
Comamonas testosteroni
1.5
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant Y155F
Comamonas testosteroni
18.9
-
androsterone
pH 9.0, 25C, recombinant His-tagged mutant K159A
Comamonas testosteroni
318
-
androsterone
pH 9.0, 25C, recombinant His-tagged wild-type
Comamonas testosteroni
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Comamonas testosteroni
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
11
pH profile, recombinant His-tagged wild-type and mutant enzymes
Comamonas testosteroni
Other publictions for EC 1.1.1.50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726505
Mundaca
Enzyme biosensor for androster ...
Comamonas testosteroni
Talanta
99
697-702
2012
-
1
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711310
Chang
Contributions of active site r ...
Comamonas testosteroni
Biochim. Biophys. Acta
1804
235-241
2010
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1
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3
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1
11
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1
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1
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4
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2
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6
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4
2
8
14
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2
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695886
Hwang
Interactions across the interf ...
Comamonas testosteroni
Arch. Biochem. Biophys.
490
36-41
2009
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1
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3
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5
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1
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1
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1
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1
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2
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5
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2
2
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5
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-
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-
-
-
-
-
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696459
Chang
Role of S114 in the NADH-induc ...
Comamonas testosteroni
Biochim. Biophys. Acta
1794
1459-1466
2009
-
-
1
-
1
-
1
6
-
-
-
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1
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1
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-
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2
-
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2
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2
2
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1
2
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1
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1
2
6
-
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-
-
1
-
-
-
-
2
-
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-
-
2
-
-
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-
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-
4
4
696786
Hidai
The Del1 deposition domain can ...
Homo sapiens, Mus musculus
Bioprocess Biosyst. Eng.
32
569-573
2009
-
1
1
-
-
-
-
-
-
-
2
-
-
2
-
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1
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1
1
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1
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1
1
1
-
-
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2
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1
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1
1
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-
-
-
-
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-
-
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697268
Xiong
3alpha-Hydroxysteroid dehydrog ...
Comamonas testosteroni
Chem. Biol. Interact.
178
206-210
2009
1
-
-
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-
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1
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1
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-
-
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697270
Xiong
Cis- and trans-regulatory elem ...
Comamonas testosteroni
Chem. Biol. Interact.
178
215-220
2009
-
2
-
-
-
-
-
-
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2
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-
-
-
-
-
-
1
1
1
1
-
-
685529
Uwai
Electronic effects of para-sub ...
Rattus norvegicus
Bioorg. Med. Chem.
16
1084-1089
2008
-
-
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1
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1
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1
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8
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1
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1
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688137
Azzarello
Tissue distribution of human A ...
Homo sapiens, Rattus norvegicus
J. Histochem. Cytochem.
56
853-861
2008
-
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1
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-
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6
-
2
2
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2
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14
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2
1
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1
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2
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14
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2
1
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688983
Wang
AKR1C2 and AKR1C3 mediated pro ...
Homo sapiens
Mol. Cell. Endocrinol.
289
60-66
2008
-
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1
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1
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689076
Stayrook
Regulation of human 3 alpha-hy ...
Homo sapiens
Mol. Pharmacol.
73
607-612
2008
1
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1
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1
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689169
Meyer
The biological activity of 3al ...
Rattus norvegicus
Neurobiol. Dis.
30
30-41
2008
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1
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1
1
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1
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1
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1
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2
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1
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684676
Sanai
Rat NAD+-dependent 3alpha-hydr ...
Rattus norvegicus
Arch. Biochem. Biophys.
464
122-129
2007
-
-
1
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3
-
1
9
2
-
2
-
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1
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1
1
-
2
2
-
31
1
1
-
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9
1
1
-
4
1
-
-
-
-
1
4
-
3
-
-
1
1
9
2
-
2
-
-
-
-
1
-
2
2
-
31
1
1
-
-
9
1
1
-
-
-
-
-
-
-
-
687635
Cooper
Elucidation of a complete kine ...
Rattus norvegicus
J. Biol. Chem.
282
33484-33493
2007
-
-
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3
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1
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1
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1
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1
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1
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1
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1
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2
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2
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3
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1
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1
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1
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1
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2
1
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687642
Chang
Mechanism of proton transfer i ...
Comamonas testosteroni
J. Biol. Chem.
282
34306-34314
2007
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1
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2
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3
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1
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1
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1
1
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1
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1
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1
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2
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1
2
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2
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3
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1
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1
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1
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2
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1
-
-
-
1
-
-
-
-
-
-
-
-
-
687870
Hoffmann
Understanding oligomerization ...
Comamonas testosteroni
J. Biotechnol.
129
131-139
2007
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
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-
-
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-
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1
-
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-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671112
Kataoka
Crystallization and preliminar ...
Pseudomonas sp., Pseudomonas sp. B-0831
Acta Crystallogr. Sect. F
62
569-571
2006
-
-
1
1
-
-
-
-
-
-
1
-
-
6
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672088
Jin
Multiple steps determine the o ...
Homo sapiens
Biochemistry
45
13054-13063
2006
-
-
1
-
-
-
-
3
-
-
-
1
-
1
-
-
1
-
-
1
-
-
3
-
1
-
-
2
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
-
-
1
-
-
-
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-
1
-
-
3
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
673349
Papari-Zareei
Arginine 276 controls the dire ...
Rattus norvegicus
Endocrinology
147
1591-1597
2006
-
-
1
-
3
-
-
1
-
-
-
1
-
3
-
-
-
1
-
1
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674674
Nakamura
Apo- and holo-structures of 3a ...
Pseudomonas sp., Pseudomonas sp. B-0831
J. Biol. Chem.
281
31876-31884
2006
-
-
-
1
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674981
Okochi
Enhanced activity of 3alpha-hy ...
Comamonas testosteroni
J. Biotechnol.
128
376-382
2006
3
1
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
1
-
-
-
1
-
-
2
-
-
-
3
1
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
675595
Steckelbroeck
Tibolone metabolism in human l ...
Homo sapiens
J. Pharmacol. Exp. Ther.
316
1300-1309
2006
-
-
-
-
-
-
3
-
2
-
-
1
-
1
-
-
-
-
-
3
1
-
3
-
1
-
-
-
1
-
-
1
-
-
2
-
-
-
1
-
-
-
2
3
-
-
2
-
-
1
-
-
-
-
-
3
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
676812
Yee
Fluorogenic metabolic probes f ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
103
13304-13309
2006
-
-
1
-
-
-
6
2
-
-
-
2
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
-
5
-
-
1
1
-
-
-
5
6
-
2
-
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669389
Hwang
Mechanistic roles of Ser-114, ...
Comamonas testosteroni
J. Biol. Chem.
280
3522-3528
2005
-
-
1
-
5
-
-
7
-
-
1
1
-
1
-
-
1
1
-
-
1
-
2
1
1
-
-
6
1
1
-
2
-
-
-
-
-
1
2
-
5
-
-
-
-
7
-
-
1
1
-
-
-
1
-
-
1
-
2
1
1
-
-
6
1
1
-
-
-
-
-
-
-
-
654186
Ueda
Kinetic study of the enzymatic ...
Pseudomonas sp., Pseudomonas sp. B-0831
Anal. Biochem.
332
84-89
2004
-
-
1
-
-
-
-
1
-
-
-
-
-
6
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
4
-
-
-
-
-
1
4
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
655790
Yee
New tools for molecular imagin ...
Comamonas testosteroni, Homo sapiens, Rattus norvegicus
J. Am. Chem. Soc.
126
2282-2283
2004
-
-
-
-
-
-
-
2
-
-
-
1
-
3
-
-
-
-
-
-
3
-
4
-
-
-
-
2
-
-
-
6
-
-
-
-
-
-
6
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
3
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
656200
Steckelbroeck
Human cytosolic 3alpha-hydroxy ...
Homo sapiens
J. Biol. Chem.
279
10784-10795
2004
-
-
1
-
-
-
2
-
1
-
-
4
-
2
-
-
1
-
-
1
4
-
7
-
1
-
-
4
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
2
-
-
1
-
-
4
-
-
-
1
-
1
4
-
7
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
656300
Ueda
-
Steady-state kinetic propertie ...
Pseudomonas sp., Pseudomonas sp. B-0831
J. Biol. Macromol.
4
23-28
2004
-
-
-
-
-
-
-
11
-
-
-
-
-
6
-
-
-
-
-
-
-
-
15
-
1
-
-
10
1
-
-
5
-
-
-
-
-
-
5
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
15
-
1
-
-
10
1
-
-
-
-
-
-
-
-
-
656787
Penning
Structure-function of human 3a ...
Homo sapiens
Mol. Cell. Endocrinol.
215
63-72
2004
-
-
1
-
-
-
1
12
-
-
-
3
-
3
-
-
-
1
-
5
1
-
13
-
1
-
-
14
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
12
-
-
-
3
-
-
-
-
-
5
1
-
13
-
1
-
-
14
1
-
-
-
-
-
-
-
-
-
656838
Steckelbroeck
Tibolone is metabolized by the ...
Homo sapiens
Mol. Pharmacol.
66
1702-1711
2004
-
-
1
-
-
-
2
1
-
-
-
1
-
3
-
-
1
-
-
1
6
-
7
-
1
-
-
6
1
-
-
4
-
-
-
-
-
1
4
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
-
1
6
-
7
-
1
-
-
6
1
-
-
-
-
-
-
-
-
-
655214
Jin
Examination of the differences ...
Homo sapiens, Rattus norvegicus
Chem. Biol. Interact.
143-144
383-392
2003
-
1
1
2
-
-
-
1
-
-
-
4
-
4
-
-
-
2
-
2
2
-
7
1
-
-
-
2
-
-
-
4
-
-
-
-
1
1
4
2
-
-
-
-
-
1
-
-
-
4
-
-
-
-
-
2
2
-
7
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
655215
Heredia
Steroid-binding site residues ...
Rattus norvegicus
Chem. Biol. Interact.
143-144
393-400
2003
-
-
-
-
8
-
-
1
-
-
-
1
-
1
-
-
-
1
-
1
-
-
4
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
8
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
655348
Rizner
Human type 3 3alpha-hydroxyste ...
Homo sapiens
Endocrinology
144
2922-2932
2003
-
-
1
-
-
-
1
3
-
-
-
1
-
2
-
-
-
-
-
1
1
-
2
-
1
-
-
3
1
-
-
4
-
-
-
-
-
1
4
-
-
-
-
1
-
3
-
-
-
1
-
-
-
-
-
1
1
-
2
-
1
-
-
3
1
-
-
-
-
-
-
-
-
-
656651
Penning
Structure-function relationshi ...
Homo sapiens, Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
85
247-255
2003
-
-
2
1
7
-
2
2
-
-
-
2
-
2
-
-
1
1
-
8
2
-
9
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
1
1
7
-
-
2
-
2
-
-
-
2
-
-
-
1
-
8
2
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654626
Trauger
Kinetics of allopregnanolone f ...
Homo sapiens
Biochemistry
41
13451-13459
2002
2
-
1
-
-
-
1
6
-
-
-
1
-
2
-
-
1
1
-
-
-
-
3
-
1
-
-
5
1
-
-
1
5
-
-
2
-
1
1
-
-
-
-
1
5
6
-
-
-
1
-
-
-
1
-
-
-
-
3
-
1
-
-
5
1
-
-
-
-
-
-
-
-
-
654924
Usami
Substrate specificity of human ...
Homo sapiens
Biol. Pharm. Bull.
25
441-445
2002
-
-
-
-
-
-
8
4
-
-
-
1
-
1
-
-
-
1
-
-
-
-
4
-
1
-
-
4
1
-
-
1
10
-
-
-
-
-
1
-
-
-
-
8
10
4
-
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
656642
Gao
Hypothalamic 5alpha-reductase ...
Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
80
91-98
2002
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
1
1
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
1
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
656648
Lou
Intestinal and hepatic microso ...
Ictalurus punctatus
J. Steroid Biochem. Mol. Biol.
82
413-424
2002
-
-
-
-
-
-
-
2
2
-
-
-
-
4
-
-
-
-
-
5
3
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
5
3
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
389541
Chetyrkin
Characterization of a novel ty ...
Homo sapiens
J. Biol. Chem.
276
22278-22286
2001
-
-
1
-
-
-
-
3
1
-
-
-
-
1
-
-
1
-
-
1
2
-
7
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
3
1
-
-
-
-
-
-
1
-
1
2
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389550
Jin
Crystal structure of human typ ...
Homo sapiens
Biochemistry
40
10161-10168
2001
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389551
Zhu
Crystallization and preliminar ...
Homo sapiens
Acta Crystallogr. Sect. D
57
589-591
2001
-
-
-
1
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389546
Maser
Functional expression, purific ...
Comamonas testosteroni
Biochem. Biophys. Res. Commun.
272
622-628
2000
-
-
1
-
-
-
-
5
-
-
2
-
-
1
-
-
1
-
-
-
5
-
12
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
-
-
2
-
-
-
-
1
-
-
5
-
12
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389552
Gao
Purification and properties of ...
Pseudomonas sp.
Ann. N. Y. Acad. Sci.
864
383-392
1998
-
1
-
-
-
-
-
1
1
-
1
1
-
1
-
-
1
-
-
-
-
-
1
-
1
1
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
1
1
-
-
-
1
-
-
-
-
1
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
389540
Biswas
Expression cloning and charact ...
Homo sapiens, Rattus norvegicus
J. Biol. Chem.
272
15959-15966
1997
-
-
2
-
-
-
-
6
-
-
-
2
-
2
-
-
-
-
-
4
2
-
16
-
-
-
-
5
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
6
-
-
-
2
-
-
-
-
-
4
2
-
16
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
389547
Lin
Expression and characterizatio ...
Homo sapiens
Mol. Endocrinol.
11
1971-1984
1997
-
-
1
-
-
-
-
10
-
-
-
1
-
4
-
-
1
1
-
12
10
-
13
-
-
-
-
11
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
10
-
-
-
1
-
-
-
1
-
12
10
-
13
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
389548
Adachi
-
Purification and properties of ...
Pseudomonas putida
Biosci. Biotechnol. Biochem.
61
131-137
1997
-
-
-
1
-
1
-
3
1
-
1
1
-
1
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1
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1
2
9
1
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1
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1
-
1
1
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-
1
1
-
1
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-
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3
1
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1
1
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1
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1
2
9
1
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-
1
-
1
-
1
-
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-
-
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-
389557
Li
Regional distribution of cytos ...
Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
60
311-318
1997
-
-
-
-
-
-
-
-
2
-
-
3
-
3
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1
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6
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2
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2
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2
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1
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6
-
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389563
Lindemann
-
Biosynthesis of pregnane deriv ...
Digitalis lanata
Phytochemistry
46
507-513
1997
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
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1
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2
1
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2
-
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-
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-
-
-
-
389562
Stuhlemmer
-
Cardenolide formation and acti ...
Digitalis lanata
Plant Physiol. Biochem.
34
85-91
1996
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
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3
2
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3
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1
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1
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1
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1
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1
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1
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3
2
-
3
-
1
-
-
-
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-
-
-
-
-
-
-
-
-
347986
Mallonee
Expression in Escherichia coli ...
Eubacterium sp., Eubacterium sp. VPI 12708
Curr. Microbiol.
30
259-263
1995
-
-
1
-
-
-
-
-
-
-
1
-
-
14
-
-
1
-
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2
1
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2
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1
2
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1
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-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389560
Seitz
-
Enzymes in cardenolide-accumul ...
Digitalis purpurea
Plant Cell Tissue Organ Cult.
38
337-344
1994
-
-
-
-
-
-
-
4
2
-
-
2
-
1
-
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1
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3
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3
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3
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4
2
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2
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1
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3
-
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-
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-
-
-
-
-
-
-
-
389559
Karavolas
Changes in pituitary, hypothal ...
Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
44
299-303
1993
-
-
-
-
-
-
-
-
2
-
-
3
-
1
-
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3
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5
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2
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3
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5
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-
-
-
-
-
389545
Iyer
Purification and properties of ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
43
343-349
1992
-
-
-
-
-
1
4
1
2
-
1
-
-
2
-
-
1
-
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2
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6
1
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1
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1
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1
-
-
1
-
4
-
1
2
-
1
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1
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2
-
-
6
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
389549
Nanjo
Characterization of bovine liv ...
Bos taurus
Int. J. Biochem.
24
815-820
1992
-
-
-
-
-
-
7
-
2
-
1
1
-
3
-
-
1
-
-
1
1
-
13
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
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7
-
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2
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1
1
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-
1
-
1
1
-
13
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389554
Pawlowski
Isolation and partial characte ...
Rattus norvegicus
Agents Actions
34
289-293
1991
-
-
1
-
-
-
-
-
2
-
1
-
-
3
-
-
-
-
-
2
-
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1
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1
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-
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2
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1
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2
-
-
-
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
389544
Campbell
Purification of the NADPH:5alp ...
Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
37
215-222
1990
-
-
-
-
-
-
-
4
2
-
1
-
-
2
-
-
1
-
-
2
2
-
2
1
-
-
-
-
-
-
-
1
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-
1
-
-
-
-
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4
2
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1
-
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1
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2
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389555
Sugimoto
In vitro metabolism of testost ...
Gallus gallus
J. Steroid Biochem.
35
271-279
1990
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
1
-
-
1
-
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1
1
1
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-
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-
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-
-
-
-
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-
1
1
-
1
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1
-
1
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
587068
Morikawa
Purification and characterizat ...
Gallus gallus
J. Steroid Biochem. Mol. Biol.
37
569-574
1990
-
-
-
-
-
1
-
1
2
-
1
-
-
2
-
-
1
-
-
3
1
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
1
2
-
1
-
-
-
-
1
-
3
1
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
389527
Hara
Purification and characterizat ...
Mus musculus
J. Biochem.
103
1027-1034
1988
-
-
-
-
-
1
28
11
2
-
2
-
-
3
-
-
1
-
-
2
1
1
12
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
28
-
11
2
-
2
-
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-
1
-
2
1
1
12
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
389556
Stolz
3alpha-hydroxysteroid dehydrog ...
Rattus norvegicus
J. Clin. Invest.
79
427-434
1987
-
-
-
-
-
-
1
-
2
-
1
1
-
2
-
-
1
-
-
3
-
-
2
-
-
-
-
-
1
-
-
1
-
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-
1
-
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1
-
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2
-
1
1
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1
-
3
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
349245
Boutin
Rat liver microsomal 3alpha-hy ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
135
795-801
1986
-
-
-
-
-
-
1
-
1
-
-
-
-
2
-
-
1
-
-
2
1
-
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-
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1
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1
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1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389525
Penning
Purification and properties of ...
Rattus norvegicus
J. Biol. Chem.
260
15266-15272
1985
-
-
-
-
-
-
9
1
2
-
2
1
-
3
-
-
1
-
-
2
1
1
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
1
2
-
2
1
-
-
-
1
-
2
1
1
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389526
Ikeda
Purification and characterizat ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
365
377-391
1984
-
-
-
-
-
-
2
1
2
-
1
-
-
2
-
-
1
-
-
2
-
1
5
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
2
-
1
-
-
-
-
1
-
2
-
1
5
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
389561
Bertics
Potent inhibition of the hypot ...
Rattus norvegicus
J. Biol. Chem.
259
107-111
1984
-
-
-
-
-
-
1
-
3
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389528
Hastings
Kinetic properties of the solu ...
Rattus norvegicus
J. Steroid Biochem.
14
705-711
1981
-
-
-
-
-
-
1
2
1
-
-
1
-
2
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
1
-
-
1
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389558
Krause
Subcellular location of hypoth ...
Rattus norvegicus
J. Steroid Biochem.
13
271-280
1980
-
-
-
-
-
-
-
-
2
-
-
2
-
1
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347740
MacDonald
Bile salt 3alpha- and 12alpha- ...
Eggerthella lenta
Appl. Environ. Microbiol.
37
992-1000
1979
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389522
Uwajima
-
Properties of crystalline 3alp ...
Pseudomonas putida
Agric. Biol. Chem.
43
1521-1528
1979
-
-
-
1
-
1
2
2
-
-
1
-
-
1
-
-
-
-
-
-
-
1
12
1
-
-
1
-
1
-
1
1
-
-
-
-
-
-
1
1
-
1
-
2
-
2
-
-
1
-
-
-
-
-
-
-
-
1
12
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
389523
Uwajima
-
Production, purification and c ...
Pseudomonas putida
Agric. Biol. Chem.
42
1577-1583
1978
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347742
Mahony
Bile salt degradation by nonfe ...
Asaccharospora irregularis
Appl. Environ. Microbiol.
34
419-423
1977
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347743
MacDonald
NAD-dependent 3alpha- and 12al ...
Eggerthella lenta
Biochim. Biophys. Acta
489
466-476
1977
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
1
7
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
1
7
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
389529
Jacobi
Characterization of the 3alpha ...
Canis lupus familiaris
J. Steroid Biochem.
8
719-723
1977
-
-
-
-
-
-
17
1
2
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
17
-
1
2
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389531
Inano
Prostate 3alpha-hydroxysteroid ...
Rattus norvegicus
J. Steroid Biochem.
8
41-46
1977
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
1
1
-
2
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
1
1
-
2
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
389532
Golf
Solubilization and purificatio ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
357
35-40
1976
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389530
Nimrod
Separation and characterizatio ...
Rattus norvegicus
J. Steroid Biochem.
6
1205-1209
1975
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389533
Skalhegg
3alpha-Hydroxysteroid dehydrog ...
Comamonas testosteroni
Eur. J. Biochem.
50
603-609
1975
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389524
Skalhegg
On the 3alpha-hydroxysteroid d ...
Comamonas testosteroni
Eur. J. Biochem.
46
117-125
1974
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389536
Squire
Physical and chemical characte ...
Comamonas testosteroni
Biochim. Biophys. Acta
89
409-421
1964
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389534
Jarabak
Stereospecificity of hydrogen ...
Comamonas testosteroni
J. Biol. Chem.
235
2147-2151
1960
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