BRENDA - Enzyme Database show
show all sequences of 1.1.1.50

Rat NAD+-dependent 3alpha-hydroxysteroid dehydrogenase (AKR1C17): a member of the aldo-keto reductase family highly expressed in kidney cytosol

Sanai, M.; Endo, S.; Matsunaga, T.; Ishikura, S.; Tajima, K.; El-Kabbani, O.; Hara, A.; Arch. Biochem. Biophys. 464, 122-129 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of wild-type and mutant AKR1C17s in Escherichia coli strain BL21(DE3)
Rattus norvegicus
Engineering
Amino acid exchange
Commentary
Organism
E276R
site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKR1C9 and its cofactor specificity, overview
Rattus norvegicus
Q270K
site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKR1C9 and its cofactor specificity, overview
Rattus norvegicus
Q270K/E276R
site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKR1C9 and its cofactor specificity, overview
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ketamine
inhibition of AKR1C17
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
cofactor kinetics of wild-type and mutant enzymes, overview
Rattus norvegicus
0.0021
-
5beta-androstane-3,17-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.0024
-
5beta-pregnane-3,20-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.0027
-
(5beta,20R)-20-hydroxypregnan-3-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.004
-
Dehydrolithocholic acid
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.022
-
isatin
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.023
-
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.038
-
9,10-phenanthrenequinone
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.42
-
diacetyl
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
AKR1C17 and AKR1C9
Rattus norvegicus
5829
-
additional information
subcellular localization of isozymes, overview
Rattus norvegicus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
recombinant AKR1C17, gel filtration
Rattus norvegicus
37000
-
1 * 37000, recombinant AKR1C17, SDS-PAGE
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
female Wistar rats
-
Purification (Commentary)
Commentary
Organism
recombinant wild-type and mutant AKR1C17s from Escherichia coli strain BL21(DE3)
Rattus norvegicus
Reaction
Reaction
Commentary
Organism
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+
the enzyme shows a sequential ordered reaction mechanism, where NAD+ binds to the enzyme first and NADH leaves last
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
specific expression of AKR1C17, two enzymes are expressed more highly in renal cortex than in the medulla
Rattus norvegicus
-
additional information
tissue distribution of isozymes, overview
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.07
-
recombinant AKR1C17 in cell extract, substrate 4-androsten-3alpha-ol-17-one
Rattus norvegicus
0.23
-
purified recombinant AKR1C17, substrate 4-androsten-3alpha-ol-17-one
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-androsten-3alpha-ol-17-one + NADH + H+
AKR1C17
684676
Rattus norvegicus
4-androsten-3alpha,17beta-diol + NAD+
-
-
-
r
4-androstene-3,17-dione + NADH + H+
low activity
684676
Rattus norvegicus
4-androsten-3alpha-ol-17-one + NAD+
-
-
-
r
5alpha-androstan-3alpha-ol-17-one + NADH + H+
-
684676
Rattus norvegicus
5beta-androstan-3alpha,17beta-diol + NAD+
-
-
-
r
5alpha-androstane-3,17-dione + NADH
low activity
684676
Rattus norvegicus
5alpha-androstan-3alpha-ol-17-one + NAD+
AKR1C9
-
-
r
5alpha-dihydrotestosterone + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
5alpha-pregnane-20alpha-ol-3-one + NADH
-
684676
Rattus norvegicus
5alpha-pregnane-3,20alpha-diol + NAD+
-
-
-
?
5alpha-pregnane-3,20-dione + NADH
low activity
684676
Rattus norvegicus
5alpha-pregnan-3alpha-ol-20-one + NAD+
-
-
-
r
5beta-androstan-3alpha-ol-17-one + NADH
-
684676
Rattus norvegicus
5beta-androstane-3alpha,17beta-diol + NAD+
-
-
-
r
5beta-androstane-3,17-dione + NADH
-
684676
Rattus norvegicus
5beta-androstan-3alpha-ol-17-one + NAD+
-
-
-
r
5beta-pregnane-20alpha-ol-3-one + NADH + H+
-
684676
Rattus norvegicus
5beta-pregnane-3,20alpha-diol + NAD+
-
-
-
?
5beta-pregnane-3,20-dione + NADH + H+
-
684676
Rattus norvegicus
5beta-pregnan-3alpha-ol-20-one + NAD+
-
-
-
r
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one + NADH
the enzyme produces both R- and S-form products in a ratio of 2.3 : 1
684676
Rattus norvegicus
?
-
-
-
?
9,10-phenanthrenequinone + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
beta-muricholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
chenodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
cholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
dehydrolithocholic acid + NADH + H+
-
684676
Rattus norvegicus
?
-
-
-
?
deoxycholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
diacetyl + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
glycochenodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
glycolithocholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
glycoursodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
hyodeoxycholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
isatin + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
lithocholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
additional information
substrate specificty, overview, recombinant AKR1C17 efficiently oxidizes 3alpha-hydroxysteroids and diverse bile acids using NAD+ as the preferred coenzyme, no activity with progesterone, cortisone, and aldosterone. The enzyme also reduces non-steroidal alpha-dicarbonyl compounds such as 9,10-phenanethrenequinone, isatin, 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one and diacetyl, but does not show significant activities towards 4-nitrobenzaldehyde and 4-nitroacetophenone
684676
Rattus norvegicus
?
-
-
-
-
murocholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
taurolithocholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
tauroursodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
testosterone + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
ursodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 37000, recombinant AKR1C17, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Rattus norvegicus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
cofactor kinetics of wild-type and mutant enzymes, overview
Rattus norvegicus
0.022
-
Dehydrolithocholic acid
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.047
-
5beta-androstane-3,17-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.1
-
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.105
-
5beta-pregnane-3,20-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.115
-
(5beta,20R)-20-hydroxypregnan-3-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.167
-
diacetyl
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.183
-
9,10-phenanthrenequinone
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.283
-
isatin
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
9.5
recombinant enzyme
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
11
-
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
preferred by AKR1C17, dependent on, residues Gln270 and Glu276 are important for cofactor specificity, overview
Rattus norvegicus
NADH
-
Rattus norvegicus
NADP+
preferred by AKR1C9, dependent on, residues Gln270 and Glu276 are important for cofactor specificity, overview
Rattus norvegicus
NADPH
-
Rattus norvegicus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
inhibition kinetics, overview
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of wild-type and mutant AKR1C17s in Escherichia coli strain BL21(DE3)
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
preferred by AKR1C17, dependent on, residues Gln270 and Glu276 are important for cofactor specificity, overview
Rattus norvegicus
NADH
-
Rattus norvegicus
NADP+
preferred by AKR1C9, dependent on, residues Gln270 and Glu276 are important for cofactor specificity, overview
Rattus norvegicus
NADPH
-
Rattus norvegicus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E276R
site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKR1C9 and its cofactor specificity, overview
Rattus norvegicus
Q270K
site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKR1C9 and its cofactor specificity, overview
Rattus norvegicus
Q270K/E276R
site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKR1C9 and its cofactor specificity, overview
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ketamine
inhibition of AKR1C17
Rattus norvegicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
inhibition kinetics, overview
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
cofactor kinetics of wild-type and mutant enzymes, overview
Rattus norvegicus
0.0021
-
5beta-androstane-3,17-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.0024
-
5beta-pregnane-3,20-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.0027
-
(5beta,20R)-20-hydroxypregnan-3-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.004
-
Dehydrolithocholic acid
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.022
-
isatin
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.023
-
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.038
-
9,10-phenanthrenequinone
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.42
-
diacetyl
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
AKR1C17 and AKR1C9
Rattus norvegicus
5829
-
additional information
subcellular localization of isozymes, overview
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
recombinant AKR1C17, gel filtration
Rattus norvegicus
37000
-
1 * 37000, recombinant AKR1C17, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant wild-type and mutant AKR1C17s from Escherichia coli strain BL21(DE3)
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
specific expression of AKR1C17, two enzymes are expressed more highly in renal cortex than in the medulla
Rattus norvegicus
-
additional information
tissue distribution of isozymes, overview
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.07
-
recombinant AKR1C17 in cell extract, substrate 4-androsten-3alpha-ol-17-one
Rattus norvegicus
0.23
-
purified recombinant AKR1C17, substrate 4-androsten-3alpha-ol-17-one
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-androsten-3alpha-ol-17-one + NADH + H+
AKR1C17
684676
Rattus norvegicus
4-androsten-3alpha,17beta-diol + NAD+
-
-
-
r
4-androstene-3,17-dione + NADH + H+
low activity
684676
Rattus norvegicus
4-androsten-3alpha-ol-17-one + NAD+
-
-
-
r
5alpha-androstan-3alpha-ol-17-one + NADH + H+
-
684676
Rattus norvegicus
5beta-androstan-3alpha,17beta-diol + NAD+
-
-
-
r
5alpha-androstane-3,17-dione + NADH
low activity
684676
Rattus norvegicus
5alpha-androstan-3alpha-ol-17-one + NAD+
AKR1C9
-
-
r
5alpha-dihydrotestosterone + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
5alpha-pregnane-20alpha-ol-3-one + NADH
-
684676
Rattus norvegicus
5alpha-pregnane-3,20alpha-diol + NAD+
-
-
-
?
5alpha-pregnane-3,20-dione + NADH
low activity
684676
Rattus norvegicus
5alpha-pregnan-3alpha-ol-20-one + NAD+
-
-
-
r
5beta-androstan-3alpha-ol-17-one + NADH
-
684676
Rattus norvegicus
5beta-androstane-3alpha,17beta-diol + NAD+
-
-
-
r
5beta-androstane-3,17-dione + NADH
-
684676
Rattus norvegicus
5beta-androstan-3alpha-ol-17-one + NAD+
-
-
-
r
5beta-pregnane-20alpha-ol-3-one + NADH + H+
-
684676
Rattus norvegicus
5beta-pregnane-3,20alpha-diol + NAD+
-
-
-
?
5beta-pregnane-3,20-dione + NADH + H+
-
684676
Rattus norvegicus
5beta-pregnan-3alpha-ol-20-one + NAD+
-
-
-
r
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one + NADH
the enzyme produces both R- and S-form products in a ratio of 2.3 : 1
684676
Rattus norvegicus
?
-
-
-
?
9,10-phenanthrenequinone + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
beta-muricholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
chenodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
cholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
dehydrolithocholic acid + NADH + H+
-
684676
Rattus norvegicus
?
-
-
-
?
deoxycholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
diacetyl + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
glycochenodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
glycolithocholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
glycoursodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
hyodeoxycholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
isatin + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
lithocholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
additional information
substrate specificty, overview, recombinant AKR1C17 efficiently oxidizes 3alpha-hydroxysteroids and diverse bile acids using NAD+ as the preferred coenzyme, no activity with progesterone, cortisone, and aldosterone. The enzyme also reduces non-steroidal alpha-dicarbonyl compounds such as 9,10-phenanethrenequinone, isatin, 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one and diacetyl, but does not show significant activities towards 4-nitrobenzaldehyde and 4-nitroacetophenone
684676
Rattus norvegicus
?
-
-
-
-
murocholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
taurolithocholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
tauroursodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
testosterone + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
ursodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 37000, recombinant AKR1C17, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Rattus norvegicus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
cofactor kinetics of wild-type and mutant enzymes, overview
Rattus norvegicus
0.022
-
Dehydrolithocholic acid
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.047
-
5beta-androstane-3,17-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.1
-
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.105
-
5beta-pregnane-3,20-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.115
-
(5beta,20R)-20-hydroxypregnan-3-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.167
-
diacetyl
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.183
-
9,10-phenanthrenequinone
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.283
-
isatin
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
9.5
recombinant enzyme
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
11
-
Rattus norvegicus
Other publictions for EC 1.1.1.50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726505
Mundaca
Enzyme biosensor for androster ...
Comamonas testosteroni
Talanta
99
697-702
2012
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711310
Chang
Contributions of active site r ...
Comamonas testosteroni
Biochim. Biophys. Acta
1804
235-241
2010
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695886
Hwang
Interactions across the interf ...
Comamonas testosteroni
Arch. Biochem. Biophys.
490
36-41
2009
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696459
Chang
Role of S114 in the NADH-induc ...
Comamonas testosteroni
Biochim. Biophys. Acta
1794
1459-1466
2009
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1
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1
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6
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6
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4
4
696786
Hidai
The Del1 deposition domain can ...
Homo sapiens, Mus musculus
Bioprocess Biosyst. Eng.
32
569-573
2009
-
1
1
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697268
Xiong
3alpha-Hydroxysteroid dehydrog ...
Comamonas testosteroni
Chem. Biol. Interact.
178
206-210
2009
1
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697270
Xiong
Cis- and trans-regulatory elem ...
Comamonas testosteroni
Chem. Biol. Interact.
178
215-220
2009
-
2
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1
1
1
1
-
-
685529
Uwai
Electronic effects of para-sub ...
Rattus norvegicus
Bioorg. Med. Chem.
16
1084-1089
2008
-
-
-
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1
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1
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8
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1
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1
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-
688137
Azzarello
Tissue distribution of human A ...
Homo sapiens, Rattus norvegicus
J. Histochem. Cytochem.
56
853-861
2008
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1
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6
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2
2
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2
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14
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2
1
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1
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6
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2
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14
-
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2
1
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-
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-
-
-
688983
Wang
AKR1C2 and AKR1C3 mediated pro ...
Homo sapiens
Mol. Cell. Endocrinol.
289
60-66
2008
-
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1
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1
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689076
Stayrook
Regulation of human 3 alpha-hy ...
Homo sapiens
Mol. Pharmacol.
73
607-612
2008
1
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1
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689169
Meyer
The biological activity of 3al ...
Rattus norvegicus
Neurobiol. Dis.
30
30-41
2008
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1
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2
1
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684676
Sanai
Rat NAD+-dependent 3alpha-hydr ...
Rattus norvegicus
Arch. Biochem. Biophys.
464
122-129
2007
-
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1
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3
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1
9
2
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2
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1
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1
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2
2
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31
1
1
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9
1
1
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4
1
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1
4
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3
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1
1
9
2
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2
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1
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2
2
-
31
1
1
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9
1
1
-
-
-
-
-
-
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-
687635
Cooper
Elucidation of a complete kine ...
Rattus norvegicus
J. Biol. Chem.
282
33484-33493
2007
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-
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3
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2
1
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687642
Chang
Mechanism of proton transfer i ...
Comamonas testosteroni
J. Biol. Chem.
282
34306-34314
2007
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1
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2
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3
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687870
Hoffmann
Understanding oligomerization ...
Comamonas testosteroni
J. Biotechnol.
129
131-139
2007
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671112
Kataoka
Crystallization and preliminar ...
Pseudomonas sp., Pseudomonas sp. B-0831
Acta Crystallogr. Sect. F
62
569-571
2006
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1
1
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6
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672088
Jin
Multiple steps determine the o ...
Homo sapiens
Biochemistry
45
13054-13063
2006
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3
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3
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2
1
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673349
Papari-Zareei
Arginine 276 controls the dire ...
Rattus norvegicus
Endocrinology
147
1591-1597
2006
-
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1
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3
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1
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3
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1
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674674
Nakamura
Apo- and holo-structures of 3a ...
Pseudomonas sp., Pseudomonas sp. B-0831
J. Biol. Chem.
281
31876-31884
2006
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1
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2
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2
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2
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674981
Okochi
Enhanced activity of 3alpha-hy ...
Comamonas testosteroni
J. Biotechnol.
128
376-382
2006
3
1
-
-
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1
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1
1
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1
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1
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675595
Steckelbroeck
Tibolone metabolism in human l ...
Homo sapiens
J. Pharmacol. Exp. Ther.
316
1300-1309
2006
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1
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1
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3
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1
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676812
Yee
Fluorogenic metabolic probes f ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
103
13304-13309
2006
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1
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2
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1
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6
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4
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669389
Hwang
Mechanistic roles of Ser-114, ...
Comamonas testosteroni
J. Biol. Chem.
280
3522-3528
2005
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1
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5
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7
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1
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1
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1
1
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6
1
1
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2
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2
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5
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7
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1
1
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1
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2
1
1
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6
1
1
-
-
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654186
Ueda
Kinetic study of the enzymatic ...
Pseudomonas sp., Pseudomonas sp. B-0831
Anal. Biochem.
332
84-89
2004
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1
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4
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4
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655790
Yee
New tools for molecular imagin ...
Comamonas testosteroni, Homo sapiens, Rattus norvegicus
J. Am. Chem. Soc.
126
2282-2283
2004
-
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6
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4
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2
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656200
Steckelbroeck
Human cytosolic 3alpha-hydroxy ...
Homo sapiens
J. Biol. Chem.
279
10784-10795
2004
-
-
1
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2
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2
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1
4
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7
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4
1
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3
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1
3
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4
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1
4
-
7
-
1
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4
1
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-
656300
Ueda
-
Steady-state kinetic propertie ...
Pseudomonas sp., Pseudomonas sp. B-0831
J. Biol. Macromol.
4
23-28
2004
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15
-
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10
1
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5
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5
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11
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1
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1
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1
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1
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12
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3
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1
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13
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14
1
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Crystal structure of human typ ...
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Crystallization and preliminar ...
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Expression and characterizatio ...
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Purification and properties of ...
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1
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2
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Regional distribution of cytos ...
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389563
Lindemann
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Biosynthesis of pregnane deriv ...
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Phytochemistry
46
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3
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Expression in Escherichia coli ...
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Enzymes in cardenolide-accumul ...
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Isolation and partial characte ...
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Purification of the NADPH:5alp ...
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Purification and characterizat ...
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-
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1
-
3
1
1
1
1
1
-
-
-
-
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389527
Hara
Purification and characterizat ...
Mus musculus
J. Biochem.
103
1027-1034
1988
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1
28
11
2
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2
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-
3
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1
-
-
2
1
1
12
1
-
1
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-
3
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-
-
-
-
-
1
-
28
-
11
2
-
2
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-
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-
1
-
2
1
1
12
1
-
1
-
-
3
-
-
-
-
-
-
-
-
-
389556
Stolz
3alpha-hydroxysteroid dehydrog ...
Rattus norvegicus
J. Clin. Invest.
79
427-434
1987
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-
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1
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2
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1
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2
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1
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3
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2
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1
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1
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1
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1
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2
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1
1
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1
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3
-
-
2
-
-
-
-
-
1
-
-
-
-
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-
-
-
-
349245
Boutin
Rat liver microsomal 3alpha-hy ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
135
795-801
1986
-
-
-
-
-
-
1
-
1
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2
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1
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2
1
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1
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1
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1
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2
1
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-
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389525
Penning
Purification and properties of ...
Rattus norvegicus
J. Biol. Chem.
260
15266-15272
1985
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-
-
-
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-
9
1
2
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2
1
-
3
-
-
1
-
-
2
1
1
3
1
-
-
-
-
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-
-
-
-
-
-
9
-
1
2
-
2
1
-
-
-
1
-
2
1
1
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389526
Ikeda
Purification and characterizat ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
365
377-391
1984
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-
-
-
-
-
2
1
2
-
1
-
-
2
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-
1
-
-
2
-
1
5
-
-
-
-
2
2
-
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-
-
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-
-
-
-
-
-
2
-
1
2
-
1
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-
-
1
-
2
-
1
5
-
-
-
-
2
2
-
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-
-
-
-
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389561
Bertics
Potent inhibition of the hypot ...
Rattus norvegicus
J. Biol. Chem.
259
107-111
1984
-
-
-
-
-
-
1
-
3
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-
1
-
1
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-
1
-
-
1
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-
-
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2
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2
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1
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3
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1
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1
-
-
1
-
-
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-
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-
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-
389528
Hastings
Kinetic properties of the solu ...
Rattus norvegicus
J. Steroid Biochem.
14
705-711
1981
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-
-
-
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1
2
1
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1
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2
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-
-
4
-
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4
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1
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2
1
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1
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-
4
-
-
4
-
-
-
-
-
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-
-
-
-
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-
-
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389558
Krause
Subcellular location of hypoth ...
Rattus norvegicus
J. Steroid Biochem.
13
271-280
1980
-
-
-
-
-
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-
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2
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2
-
1
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1
1
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4
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2
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2
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2
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2
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1
1
-
4
-
-
-
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-
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-
-
-
347740
MacDonald
Bile salt 3alpha- and 12alpha- ...
Eggerthella lenta
Appl. Environ. Microbiol.
37
992-1000
1979
-
-
-
-
-
-
-
-
-
-
1
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1
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1
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-
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-
389522
Uwajima
-
Properties of crystalline 3alp ...
Pseudomonas putida
Agric. Biol. Chem.
43
1521-1528
1979
-
-
-
1
-
1
2
2
-
-
1
-
-
1
-
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-
-
-
-
-
1
12
1
-
-
1
-
1
-
1
1
-
-
-
-
-
-
1
1
-
1
-
2
-
2
-
-
1
-
-
-
-
-
-
-
-
1
12
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
389523
Uwajima
-
Production, purification and c ...
Pseudomonas putida
Agric. Biol. Chem.
42
1577-1583
1978
-
-
-
1
-
-
-
-
-
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-
1
-
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1
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-
1
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-
1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347742
Mahony
Bile salt degradation by nonfe ...
Asaccharospora irregularis
Appl. Environ. Microbiol.
34
419-423
1977
-
-
-
-
-
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-
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1
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347743
MacDonald
NAD-dependent 3alpha- and 12al ...
Eggerthella lenta
Biochim. Biophys. Acta
489
466-476
1977
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-
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3
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1
7
-
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1
-
1
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-
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3
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-
1
7
-
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-
1
-
1
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389529
Jacobi
Characterization of the 3alpha ...
Canis lupus familiaris
J. Steroid Biochem.
8
719-723
1977
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1
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1
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2
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-
17
-
1
2
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1
-
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2
-
-
-
-
-
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389531
Inano
Prostate 3alpha-hydroxysteroid ...
Rattus norvegicus
J. Steroid Biochem.
8
41-46
1977
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-
-
-
-
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-
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1
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1
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1
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1
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-
1
1
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2
-
1
-
1
-
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-
-
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-
1
-
1
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1
-
1
1
-
2
-
1
-
1
-
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-
-
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389532
Golf
Solubilization and purificatio ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
357
35-40
1976
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1
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1
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1
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1
3
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-
-
-
-
-
-
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-
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-
-
-
-
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-
1
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-
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1
-
1
-
1
3
-
-
-
-
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389530
Nimrod
Separation and characterizatio ...
Rattus norvegicus
J. Steroid Biochem.
6
1205-1209
1975
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1
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1
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2
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2
1
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-
1
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2
-
-
-
-
-
-
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-
-
-
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-
-
-
389533
Skalhegg
3alpha-Hydroxysteroid dehydrog ...
Comamonas testosteroni
Eur. J. Biochem.
50
603-609
1975
-
-
-
-
-
-
1
1
-
-
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2
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1
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1
-
1
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-
1
-
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-
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389524
Skalhegg
On the 3alpha-hydroxysteroid d ...
Comamonas testosteroni
Eur. J. Biochem.
46
117-125
1974
-
-
-
-
-
-
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2
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2
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2
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2
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2
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389536
Squire
Physical and chemical characte ...
Comamonas testosteroni
Biochim. Biophys. Acta
89
409-421
1964
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1
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1
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1
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1
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389534
Jarabak
Stereospecificity of hydrogen ...
Comamonas testosteroni
J. Biol. Chem.
235
2147-2151
1960
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1
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1
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1
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