1.1.1.50: 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific)

This is an abbreviated version, for detailed information about 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific), go to the full flat file.

Reaction

a 3alpha-hydroxysteroid
+
NAD(P)+
=
a 3-oxosteroid
+
NAD(P)H
+
H+

Synonyms

3 alpha-hydroxysteroid dehydrogenase, 3 alphaHD, 3-alpha-HSD, 3alpha-HSD, 3alpha-HSD/CR, 3alpha-HSOR, 3alpha-hydroxysteroid dehydrogenase, 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase, 3alpha-hydroxysteroid oxido-reductase, 3alpha-hydroxysteroid oxidoreductase, 3alpha-hydroxysteroid-5beta-oxidoreductase activity, 3alpha-OR, 3alpha-oxidoreductase, 3alpha/3beta-hydroxysteroid dehydrogenase, 3alphaHSD, 3HSD, 5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase, AKR1C, AKR1C1-4, AKR1C17, AKR1C2, AKR1C3, AKR1C4, AKR1C9, aldo-keto reductase 1C2, bile-acid binding protein, chlordecone reductase, DD2, DD21, dihydrodiol dehydrogenase, HSD, HSD28, HSD29, hsdA, HSDH, hydroxyprostaglandin dehydrogenase, More, NAD(P)+-3alpha-hydroxysteroid dehydrogenase, NAD+-dependent 3alpha-HSD, NADP(H)-dependent 3alpha-HSD, NADPH:5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase, prostaglandin F2alpha-synthase, PT3HSD, sterognost 3alpha dehydrogenase, 3alpha-hydroxy steroid, type 2 3alpha-hydroxysteroid dehydrogenase/type 5 17beta-hydroxysteroid dehydrogenase, type 3 3alpha-hydroxysteroid dehydrogenase, type 5 17beta-hydroxysteroid dehydrogenase, type I 3alpha-HSD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.50 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific)

KM Value

KM Value on EC 1.1.1.50 - 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific)

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.017 - 0.042
(2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
0.069
(3alpha,5beta,7beta)-3,7-dihydroxycholan-24-oic acid
-
pH 8.5, 37°C, with cofactor NAD+
0.0027
(5beta,20R)-20-hydroxypregnan-3-one
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.022 - 0.14
1-Acenaphthenol
0.048 - 1.4
1-Indanol
0.002 - 0.0024
17beta-hydroxy-5alpha-androstan-3-one
0.0007 - 0.0017
17beta-hydroxy-5beta-androstan-3-one
0.0007 - 0.00075
3-oxo-5beta-cholan-24-oic acid
-
-
0.0001
3alpha-adiol
-
-
-
0.0031
3alpha-androstanediol
-
pH 7.0, 25°C, recombinant enzyme
0.0014
3alpha-hydroxy-5alpha-pregnan-20-one
-
oxidation
0.005
3alpha-tetrahydroprogesterone
-
-
0.2 - 0.8
4-Nitroacetophenone
-
also in this range: 2,3-hexanedione, 1-phenyl-1,2-propanedione, 2,3-pentanedione, pyridine-4-aldehyde, 2,3-butanedione
0.087
4-Nitrobenzaldehyde
-
NADPH, detection spectrophotometrically
0.0012 - 0.007
5alpha-Androstan-3,17-dione
0.00065 - 0.0031
5alpha-androstane-3,17-dione
0.0006 - 0.026
5alpha-dihydroprogesterone
0.0001 - 0.026
5alpha-dihydrotestosterone
0.000082
5alpha-pregnan-3,20-dione
-
reduction
0.00016 - 0.0016
5beta-androstan-3,17-dione
0.0012 - 0.007
5beta-androstane-3,17-dione
0.018
5beta-Dihydrocortisol
-
-
0.00051 - 0.0012
5beta-dihydroprogesterone
0.235
5beta-Pregnan-3alpha-ol-20-one
-
cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
0.0024
5beta-pregnane-3,20-dione
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.023
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.021 - 0.038
9,10-phenanthrenequinone
0.003
9-(phenylcarbonyl)-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one
-
recombinant enzyme in intact COS-1 cells
0.0015
allopregnanolone
-
pH 7.0, 25°C
0.004 - 0.0422
androstanedione
0.0001 - 0.21
androsterone
0.017 - 1.8
benzenedihydrodiol
0.03 - 0.077
Camphorquinone
-
4-nitrobenzaldehyde, 2,3-heptanedione
0.063
chenodeoxycholic acid
-
pH 8.5, 37°C, with cofactor NAD+
0.015
cholate
-
-
0.01 - 0.084
cholic acid
0.004
Dehydrolithocholic acid
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.0136
deoxycholate
-
-
0.42
diacetyl
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.0061 - 0.037
Fusidic acid
0.038
glycocholic acid
-
pH 8.5, 37°C, with cofactor NAD+
0.0052 - 0.0059
glycolithocholic acid
0.022
isatin
-
pH 7.4, 25°C, recombinant wild-type AKR1C17
0.068
lithocholic acid
-
pH 8.5, 37°C, with cofactor NAD+
0.00305 - 0.87
NAD+
0.004 - 0.4
NADH
0.00023 - 0.0016
NADP+
0.00007 - 1.33
NADPH
0.0025
oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one
-
isozyme AKR1C3
0.043
taurocholic acid
-
pH 8.5, 37°C, with cofactor NAD+
0.0028 - 0.119
testosterone
additional information
additional information
-