General Stability | Organism |
---|---|
glycerol: 30%, partially stabilizes | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
11beta,17alpha,21-trihydroxy-4-pregnen-3,20-dione | - |
Mus musculus | |
11beta,21-dihydroxy-4-pregnen-3,20-dione | - |
Mus musculus | |
17alpha,21-Dihydroxy-1,4-pregnadien-3,11,20-trione | - |
Mus musculus | |
17alpha,21-dihydroxy-4-pregnen-3,11,20-trione | - |
Mus musculus | |
17alpha-Hydroxy-4-androsten-3-one | - |
Mus musculus | |
17alpha-hydroxy-4-pregnen-3,20-dione | - |
Mus musculus | |
17beta-hydroxy-4-androsten-3-one | - |
Mus musculus | |
2alpha-Bromo-17beta-hydroxy-5alpha-androstan-3-one | - |
Mus musculus | |
4-androsten-3,17-dione | - |
Mus musculus | |
4-pregnen-3,20-dione | - |
Mus musculus | |
6alpha-Methylprednisolone | - |
Mus musculus | |
Bethamethasone | - |
Mus musculus | |
deoxycorticosterone | - |
Mus musculus | |
dexamethasone | - |
Mus musculus | |
Dienstrol | - |
Mus musculus | |
Hexestrol | - |
Mus musculus | |
Hg2+ | - |
Mus musculus | |
indomethacin | - |
Mus musculus | |
Medroxyprogesterone acetate | - |
Mus musculus | |
additional information | product inhibition | Mus musculus | |
Prednisolone | - |
Mus musculus | |
prostaglandin A1 | - |
Mus musculus | |
Prostaglandin D2 | - |
Mus musculus | |
prostaglandin E2 | - |
Mus musculus | |
prostaglandin F2alpha | - |
Mus musculus | |
Stilbestrol | - |
Mus musculus | |
Triamcinolone | - |
Mus musculus | |
Zomepirac | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00035 | 0.00049 | 5beta-androstan-3,17-dione | also in this range: 5beta-pregnan-3,20-dione, 5alpha-androstan-3,17-dione | Mus musculus | |
0.0007 | 0.00075 | 3-oxo-5beta-cholan-24-oic acid | - |
Mus musculus | |
0.00097 | - |
NADPH | - |
Mus musculus | |
0.0012 | 0.0017 | 17beta-hydroxy-5beta-androstan-3-one | also in this range: 17alpha,21-dihydroxy-5beta-pregnan-3,11,20-trione, acenaphthequinone | Mus musculus | |
0.002 | 0.0024 | 17beta-hydroxy-5alpha-androstan-3-one | - |
Mus musculus | |
0.0052 | 0.0059 | glycolithocholic acid | - |
Mus musculus | |
0.007 | 0.0083 | 5alpha-dihydrotestosterone | also in this range: 3alpha-hydroxy-5beta-pregnan-17-one | Mus musculus | |
0.0095 | - |
androsterone | - |
Mus musculus | |
0.01 | 0.025 | cholic acid | 3alpha-hydroxy-5beta-androstan-17-one, 3alpha,21-dihydroxy-5beta-pregnan-20-one, 5beta-androstan-3alpha,17beta-diol, 5alpha-androstan-3alpha,17beta-diol, NADP+ | Mus musculus | |
0.03 | 0.077 | Camphorquinone | 4-nitrobenzaldehyde, 2,3-heptanedione | Mus musculus | |
0.2 | 0.8 | 4-Nitroacetophenone | also in this range: 2,3-hexanedione, 1-phenyl-1,2-propanedione, 2,3-pentanedione, pyridine-4-aldehyde, 2,3-butanedione | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32500 | 34000 | gel filtration | Mus musculus |
34000 | - |
1 * 34000, SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.1 | - |
- |
Mus musculus |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, at least 2 weeks, crude extract loses 80% activity at 4°C in 3 days | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3alpha,17alpha,21-trihydroxy-5beta-pregnan-11,20-dione + NADP+ | - |
Mus musculus | 17alpha,21-dihydroxy-5beta-pregnan-3,11,20-trione + NADPH | - |
r | |
3alpha,21-dihydroxy-5beta-pregnan-20-one + NADP+ | - |
Mus musculus | 21-hydroxy-5beta-pregnan-3,20-dione + NADPH | - |
r | |
3alpha-hydroxy-5beta-androstan-17-one + NADP+ | - |
Mus musculus | 5beta-androstan-3,17-dione + NADPH | - |
r | |
3alpha-hydroxy-5beta-pregnan-20-one + NADP+ | - |
Mus musculus | 5beta-pregnan-3,20-dione + NADPH | - |
r | |
4-nitroacetophenone + NADPH + H+ | - |
Mus musculus | 1-(4-nitrophenyl)ethanol + NADP+ | - |
? | |
5alpha-androstan-3alpha,17beta-diol + NAD(P)+ | - |
Mus musculus | 17beta-hydroxy-5alpha-androstan-3-one + NADPH | - |
r | |
5beta-androstan-3alpha,17beta-diol + NADP+ | - |
Mus musculus | 17beta-hydroxy-5beta-androstan-3-one + NADPH | - |
r | |
androsterone + NAD(P)+ | i.e. 3alpha-hydroxy-5alpha-androstan-17-one | Mus musculus | 5alpha-androstan-3,17-dione + NAD(P)H | - |
r | |
camphorquinone + NADPH | - |
Mus musculus | ? | - |
? | |
cholic acid + NADPH + H+ | - |
Mus musculus | ? | - |
r | |
glycolithocholic acid + NADP+ | i.e. 3alpha-hydroxy-5beta-cholanoyl glycine | Mus musculus | 3-oxo-glycocholanoic acid + NADPH | - |
? | |
additional information | reduction of carbonyl compounds e.g. quinones, aliphatic-chain diketones, ketones | Mus musculus | ? | - |
? | |
additional information | absolutely specific for 3alpha-hydroxy group of C19, C21 and C24 steroids | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, SDS-PAGE | Mus musculus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
16 | 36 | increase of activity over range | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reduction with NADPH | Mus musculus |
7 | - |
reduction of 3-oxo-steroid | Mus musculus |
9.5 | - |
oxidation of 3alpha-hydroxysteroids | Mus musculus |