BRENDA - Enzyme Database show
show all sequences of 1.1.1.50

Purification and characterization of NADP+-dependent 3alpha-hydroxysteroid dehydrogenase from mouse liver cytosol

Hara, A.; Inoue, Y.; Nakagawa, M.; Naganeo, F.; Sawada, H.; J. Biochem. 103, 1027-1034 (1988)

Data extracted from this reference:

General Stability
General Stability
Organism
glycerol: 30%, partially stabilizes
Mus musculus
Inhibitors
Inhibitors
Commentary
Organism
Structure
11beta,17alpha,21-trihydroxy-4-pregnen-3,20-dione
-
Mus musculus
11beta,21-dihydroxy-4-pregnen-3,20-dione
-
Mus musculus
17alpha,21-Dihydroxy-1,4-pregnadien-3,11,20-trione
-
Mus musculus
17alpha,21-dihydroxy-4-pregnen-3,11,20-trione
-
Mus musculus
17alpha-Hydroxy-4-androsten-3-one
-
Mus musculus
17alpha-hydroxy-4-pregnen-3,20-dione
-
Mus musculus
17beta-hydroxy-4-androsten-3-one
-
Mus musculus
2alpha-Bromo-17beta-hydroxy-5alpha-androstan-3-one
-
Mus musculus
4-androsten-3,17-dione
-
Mus musculus
4-pregnen-3,20-dione
-
Mus musculus
6alpha-Methylprednisolone
-
Mus musculus
Bethamethasone
-
Mus musculus
deoxycorticosterone
-
Mus musculus
dexamethasone
-
Mus musculus
Dienstrol
-
Mus musculus
Hexestrol
-
Mus musculus
Hg2+
-
Mus musculus
indomethacin
-
Mus musculus
Medroxyprogesterone acetate
-
Mus musculus
additional information
product inhibition
Mus musculus
Prednisolone
-
Mus musculus
prostaglandin A1
-
Mus musculus
Prostaglandin D2
-
Mus musculus
prostaglandin E2
-
Mus musculus
prostaglandin F2alpha
-
Mus musculus
Stilbestrol
-
Mus musculus
Triamcinolone
-
Mus musculus
Zomepirac
-
Mus musculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00035
0.00049
5beta-androstan-3,17-dione
also in this range: 5beta-pregnan-3,20-dione, 5alpha-androstan-3,17-dione
Mus musculus
0.0007
0.00075
3-oxo-5beta-cholan-24-oic acid
-
Mus musculus
0.00097
-
NADPH
-
Mus musculus
0.0012
0.0017
17beta-hydroxy-5beta-androstan-3-one
also in this range: 17alpha,21-dihydroxy-5beta-pregnan-3,11,20-trione, acenaphthequinone
Mus musculus
0.002
0.0024
17beta-hydroxy-5alpha-androstan-3-one
-
Mus musculus
0.0052
0.0059
glycolithocholic acid
-
Mus musculus
0.007
0.0083
5alpha-dihydrotestosterone
also in this range: 3alpha-hydroxy-5beta-pregnan-17-one
Mus musculus
0.0095
-
androsterone
-
Mus musculus
0.01
0.025
cholic acid
3alpha-hydroxy-5beta-androstan-17-one, 3alpha,21-dihydroxy-5beta-pregnan-20-one, 5beta-androstan-3alpha,17beta-diol, 5alpha-androstan-3alpha,17beta-diol, NADP+
Mus musculus
0.03
0.077
Camphorquinone
4-nitrobenzaldehyde, 2,3-heptanedione
Mus musculus
0.2
0.8
4-Nitroacetophenone
also in this range: 2,3-hexanedione, 1-phenyl-1,2-propanedione, 2,3-pentanedione, pyridine-4-aldehyde, 2,3-butanedione
Mus musculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mus musculus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32500
34000
gel filtration
Mus musculus
34000
-
1 * 34000, SDS-PAGE
Mus musculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Mus musculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Mus musculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.1
-
-
Mus musculus
Storage Stability
Storage Stability
Organism
-20°C, purified enzyme, at least 2 weeks, crude extract loses 80% activity at 4°C in 3 days
Mus musculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha,17alpha,21-trihydroxy-5beta-pregnan-11,20-dione + NADP+
-
389527
Mus musculus
17alpha,21-dihydroxy-5beta-pregnan-3,11,20-trione + NADPH
-
389527
Mus musculus
r
3alpha,21-dihydroxy-5beta-pregnan-20-one + NADP+
-
389527
Mus musculus
21-hydroxy-5beta-pregnan-3,20-dione + NADPH
-
-
-
r
3alpha-hydroxy-5beta-androstan-17-one + NADP+
-
389527
Mus musculus
5beta-androstan-3,17-dione + NADPH
-
-
-
r
3alpha-hydroxy-5beta-pregnan-20-one + NADP+
-
389527
Mus musculus
5beta-pregnan-3,20-dione + NADPH
-
-
-
r
4-nitroacetophenone + NADPH + H+
-
389527
Mus musculus
1-(4-nitrophenyl)ethanol + NADP+
-
-
-
-
5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
389527
Mus musculus
17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
389527
Mus musculus
r
5beta-androstan-3alpha,17beta-diol + NADP+
-
389527
Mus musculus
17beta-hydroxy-5beta-androstan-3-one + NADPH
-
-
-
r
androsterone + NAD(P)+
i.e. 3alpha-hydroxy-5alpha-androstan-17-one
389527
Mus musculus
5alpha-androstan-3,17-dione + NAD(P)H
-
-
-
r
camphorquinone + NADPH
-
389527
Mus musculus
?
-
-
-
-
glycolithocholic acid + NADP+
i.e. 3alpha-hydroxy-5beta-cholanoyl glycine
389527
Mus musculus
3-oxo-glycocholanoic acid + NADPH
-
-
-
?
additional information
reduction of carbonyl compounds e.g. quinones, aliphatic-chain diketones, ketones
389527
Mus musculus
?
-
-
-
-
additional information
absolutely specific for 3alpha-hydroxy group of C19, C21 and C24 steroids
389527
Mus musculus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 34000, SDS-PAGE
Mus musculus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
16
36
increase of activity over range
Mus musculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reduction with NADPH
Mus musculus
7
-
reduction of 3-oxo-steroid
Mus musculus
9.5
-
oxidation of 3alpha-hydroxysteroids
Mus musculus
General Stability (protein specific)
General Stability
Organism
glycerol: 30%, partially stabilizes
Mus musculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
11beta,17alpha,21-trihydroxy-4-pregnen-3,20-dione
-
Mus musculus
11beta,21-dihydroxy-4-pregnen-3,20-dione
-
Mus musculus
17alpha,21-Dihydroxy-1,4-pregnadien-3,11,20-trione
-
Mus musculus
17alpha,21-dihydroxy-4-pregnen-3,11,20-trione
-
Mus musculus
17alpha-Hydroxy-4-androsten-3-one
-
Mus musculus
17alpha-hydroxy-4-pregnen-3,20-dione
-
Mus musculus
17beta-hydroxy-4-androsten-3-one
-
Mus musculus
2alpha-Bromo-17beta-hydroxy-5alpha-androstan-3-one
-
Mus musculus
4-androsten-3,17-dione
-
Mus musculus
4-pregnen-3,20-dione
-
Mus musculus
6alpha-Methylprednisolone
-
Mus musculus
Bethamethasone
-
Mus musculus
deoxycorticosterone
-
Mus musculus
dexamethasone
-
Mus musculus
Dienstrol
-
Mus musculus
Hexestrol
-
Mus musculus
Hg2+
-
Mus musculus
indomethacin
-
Mus musculus
Medroxyprogesterone acetate
-
Mus musculus
additional information
product inhibition
Mus musculus
Prednisolone
-
Mus musculus
prostaglandin A1
-
Mus musculus
Prostaglandin D2
-
Mus musculus
prostaglandin E2
-
Mus musculus
prostaglandin F2alpha
-
Mus musculus
Stilbestrol
-
Mus musculus
Triamcinolone
-
Mus musculus
Zomepirac
-
Mus musculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00035
0.00049
5beta-androstan-3,17-dione
also in this range: 5beta-pregnan-3,20-dione, 5alpha-androstan-3,17-dione
Mus musculus
0.0007
0.00075
3-oxo-5beta-cholan-24-oic acid
-
Mus musculus
0.00097
-
NADPH
-
Mus musculus
0.0012
0.0017
17beta-hydroxy-5beta-androstan-3-one
also in this range: 17alpha,21-dihydroxy-5beta-pregnan-3,11,20-trione, acenaphthequinone
Mus musculus
0.002
0.0024
17beta-hydroxy-5alpha-androstan-3-one
-
Mus musculus
0.0052
0.0059
glycolithocholic acid
-
Mus musculus
0.007
0.0083
5alpha-dihydrotestosterone
also in this range: 3alpha-hydroxy-5beta-pregnan-17-one
Mus musculus
0.0095
-
androsterone
-
Mus musculus
0.01
0.025
cholic acid
3alpha-hydroxy-5beta-androstan-17-one, 3alpha,21-dihydroxy-5beta-pregnan-20-one, 5beta-androstan-3alpha,17beta-diol, 5alpha-androstan-3alpha,17beta-diol, NADP+
Mus musculus
0.03
0.077
Camphorquinone
4-nitrobenzaldehyde, 2,3-heptanedione
Mus musculus
0.2
0.8
4-Nitroacetophenone
also in this range: 2,3-hexanedione, 1-phenyl-1,2-propanedione, 2,3-pentanedione, pyridine-4-aldehyde, 2,3-butanedione
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mus musculus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32500
34000
gel filtration
Mus musculus
34000
-
1 * 34000, SDS-PAGE
Mus musculus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Mus musculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.1
-
-
Mus musculus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, purified enzyme, at least 2 weeks, crude extract loses 80% activity at 4°C in 3 days
Mus musculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3alpha,17alpha,21-trihydroxy-5beta-pregnan-11,20-dione + NADP+
-
389527
Mus musculus
17alpha,21-dihydroxy-5beta-pregnan-3,11,20-trione + NADPH
-
389527
Mus musculus
r
3alpha,21-dihydroxy-5beta-pregnan-20-one + NADP+
-
389527
Mus musculus
21-hydroxy-5beta-pregnan-3,20-dione + NADPH
-
-
-
r
3alpha-hydroxy-5beta-androstan-17-one + NADP+
-
389527
Mus musculus
5beta-androstan-3,17-dione + NADPH
-
-
-
r
3alpha-hydroxy-5beta-pregnan-20-one + NADP+
-
389527
Mus musculus
5beta-pregnan-3,20-dione + NADPH
-
-
-
r
4-nitroacetophenone + NADPH + H+
-
389527
Mus musculus
1-(4-nitrophenyl)ethanol + NADP+
-
-
-
-
5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
389527
Mus musculus
17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
389527
Mus musculus
r
5beta-androstan-3alpha,17beta-diol + NADP+
-
389527
Mus musculus
17beta-hydroxy-5beta-androstan-3-one + NADPH
-
-
-
r
androsterone + NAD(P)+
i.e. 3alpha-hydroxy-5alpha-androstan-17-one
389527
Mus musculus
5alpha-androstan-3,17-dione + NAD(P)H
-
-
-
r
camphorquinone + NADPH
-
389527
Mus musculus
?
-
-
-
-
glycolithocholic acid + NADP+
i.e. 3alpha-hydroxy-5beta-cholanoyl glycine
389527
Mus musculus
3-oxo-glycocholanoic acid + NADPH
-
-
-
?
additional information
reduction of carbonyl compounds e.g. quinones, aliphatic-chain diketones, ketones
389527
Mus musculus
?
-
-
-
-
additional information
absolutely specific for 3alpha-hydroxy group of C19, C21 and C24 steroids
389527
Mus musculus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 34000, SDS-PAGE
Mus musculus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
16
36
increase of activity over range
Mus musculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reduction with NADPH
Mus musculus
7
-
reduction of 3-oxo-steroid
Mus musculus
9.5
-
oxidation of 3alpha-hydroxysteroids
Mus musculus
Other publictions for EC 1.1.1.50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726505
Mundaca
Enzyme biosensor for androster ...
Comamonas testosteroni
Talanta
99
697-702
2012
-
1
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1
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1
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1
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1
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711310
Chang
Contributions of active site r ...
Comamonas testosteroni
Biochim. Biophys. Acta
1804
235-241
2010
-
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1
-
3
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1
11
-
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1
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2
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1
8
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4
-
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1
2
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6
-
4
2
8
14
-
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-
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2
-
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695886
Hwang
Interactions across the interf ...
Comamonas testosteroni
Arch. Biochem. Biophys.
490
36-41
2009
-
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1
-
3
-
-
5
-
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1
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1
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1
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1
-
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2
2
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5
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1
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3
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5
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1
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1
1
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2
2
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5
-
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-
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696459
Chang
Role of S114 in the NADH-induc ...
Comamonas testosteroni
Biochim. Biophys. Acta
1794
1459-1466
2009
-
-
1
-
1
-
1
6
-
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1
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1
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2
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2
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2
2
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1
2
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1
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1
2
6
-
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1
-
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-
-
2
-
-
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2
-
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4
4
696786
Hidai
The Del1 deposition domain can ...
Homo sapiens, Mus musculus
Bioprocess Biosyst. Eng.
32
569-573
2009
-
1
1
-
-
-
-
-
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2
-
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2
-
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1
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1
1
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1
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1
1
1
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2
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1
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1
1
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-
-
-
697268
Xiong
3alpha-Hydroxysteroid dehydrog ...
Comamonas testosteroni
Chem. Biol. Interact.
178
206-210
2009
1
-
-
-
-
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-
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1
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1
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697270
Xiong
Cis- and trans-regulatory elem ...
Comamonas testosteroni
Chem. Biol. Interact.
178
215-220
2009
-
2
-
-
-
-
-
-
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2
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2
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1
1
1
1
-
-
685529
Uwai
Electronic effects of para-sub ...
Rattus norvegicus
Bioorg. Med. Chem.
16
1084-1089
2008
-
-
-
-
-
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1
-
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1
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1
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8
-
1
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1
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1
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1
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1
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1
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8
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1
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1
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688137
Azzarello
Tissue distribution of human A ...
Homo sapiens, Rattus norvegicus
J. Histochem. Cytochem.
56
853-861
2008
-
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1
-
-
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-
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6
-
2
2
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2
-
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14
-
-
2
1
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1
-
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6
-
2
2
-
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-
-
-
14
-
-
2
1
-
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Crystallization and preliminar ...
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Multiple steps determine the o ...
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Papari-Zareei
Arginine 276 controls the dire ...
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Yee
Fluorogenic metabolic probes f ...
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Kinetic study of the enzymatic ...
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Steckelbroeck
Human cytosolic 3alpha-hydroxy ...
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Steady-state kinetic propertie ...
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11
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Penning
Structure-function of human 3a ...
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1
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12
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1
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13
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14
1
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Steckelbroeck
Tibolone is metabolized by the ...
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Jin
Examination of the differences ...
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2
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2
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1
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655215
Heredia
Steroid-binding site residues ...
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1
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655348
Rizner
Human type 3 3alpha-hydroxyste ...
Homo sapiens
Endocrinology
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2003
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1
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1
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2
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1
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3
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Penning
Structure-function relationshi ...
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1
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8
2
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654626
Trauger
Kinetics of allopregnanolone f ...
Homo sapiens
Biochemistry
41
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654924
Usami
Substrate specificity of human ...
Homo sapiens
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Hypothalamic 5alpha-reductase ...
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389541
Chetyrkin
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1
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1
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7
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Crystal structure of human typ ...
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Crystallization and preliminar ...
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Functional expression, purific ...
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Lindemann
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Biosynthesis of pregnane deriv ...
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Expression in Escherichia coli ...
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Purification of the NADPH:5alp ...
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Purification and characterizat ...
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Rat liver microsomal 3alpha-hy ...
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Purification and properties of ...
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Ikeda
Purification and characterizat ...
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389561
Bertics
Potent inhibition of the hypot ...
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Hastings
Kinetic properties of the solu ...
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Krause
Subcellular location of hypoth ...
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347740
MacDonald
Bile salt 3alpha- and 12alpha- ...
Eggerthella lenta
Appl. Environ. Microbiol.
37
992-1000
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389522
Uwajima
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Properties of crystalline 3alp ...
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12
1
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389523
Uwajima
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Production, purification and c ...
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347742
Mahony
Bile salt degradation by nonfe ...
Asaccharospora irregularis
Appl. Environ. Microbiol.
34
419-423
1977
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347743
MacDonald
NAD-dependent 3alpha- and 12al ...
Eggerthella lenta
Biochim. Biophys. Acta
489
466-476
1977
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3
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7
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389529
Jacobi
Characterization of the 3alpha ...
Canis lupus familiaris
J. Steroid Biochem.
8
719-723
1977
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1
2
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17
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389531
Inano
Prostate 3alpha-hydroxysteroid ...
Rattus norvegicus
J. Steroid Biochem.
8
41-46
1977
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389532
Golf
Solubilization and purificatio ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
357
35-40
1976
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3
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389530
Nimrod
Separation and characterizatio ...
Rattus norvegicus
J. Steroid Biochem.
6
1205-1209
1975
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389533
Skalhegg
3alpha-Hydroxysteroid dehydrog ...
Comamonas testosteroni
Eur. J. Biochem.
50
603-609
1975
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389524
Skalhegg
On the 3alpha-hydroxysteroid d ...
Comamonas testosteroni
Eur. J. Biochem.
46
117-125
1974
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389536
Squire
Physical and chemical characte ...
Comamonas testosteroni
Biochim. Biophys. Acta
89
409-421
1964
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389534
Jarabak
Stereospecificity of hydrogen ...
Comamonas testosteroni
J. Biol. Chem.
235
2147-2151
1960
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