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Literature summary for 1.1.1.50 extracted from

  • Papari-Zareei, M.; Brandmaier, A.; Auchus, R.J.
    Arginine 276 controls the directional preference of AKR1C9 (rat liver 3alpha-hydroxysteroid dehydrogenase) in human embryonic kidney 293 cells (2006), Endocrinology, 147, 1591-1597.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
stable expression of wild-type and mutant enzymes in HEK-293 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
R276E site-directed mutagenesis, the mutant shows increased preference for the oxidation reaction compared to the wild-type enzyme Rattus norvegicus
R276G site-directed mutagenesis, the mutant shows slightly increased preference for the reduction reaction compared to the wild-type enzyme Rattus norvegicus
R276M site-directed mutagenesis, the mutant shows slightly increased preference for the reduction reaction compared to the wild-type enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information equilibrium kinetics of wild-type and mutant enzymes Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5alpha-dihydrotestosterone + NADPH Rattus norvegicus
-
5alpha-androstane-3alpha,17beta-diol + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ the reduction reaction is slightly preferred by the wild-type enzyme, which is determined by residue Arg276 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-dihydrotestosterone + NADPH
-
Rattus norvegicus 5alpha-androstane-3alpha,17beta-diol + NADP+
-
?
5alpha-dihydrotestosterone + NADPH i.e. DHT or 5alpha-androstane-17beta-ol-3-one, the reduction reaction is slightly preferred by the wild-type enzyme, which is determined by residue Arg276 Rattus norvegicus 5alpha-androstane-3alpha,17beta-diol + NADP+ i.e. 5alpha-androstane-3alpha,17beta-diol ?

Synonyms

Synonyms Comment Organism
3alphaHSD
-
Rattus norvegicus
AKR1C9
-
Rattus norvegicus
More cf. EC 1.1.1.213 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD(P)H 1000fold lower activity with NADH compared to NADPH Rattus norvegicus