1.1.1.47: glucose 1-dehydrogenase [NAD(P)+]

This is an abbreviated version, for detailed information about glucose 1-dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

D-glucose
+
NAD(P)+
=
D-glucono-1,5-lactone
+
NAD(P)H
+
H+

Synonyms

alkali-resistant glucose 1-dehydrogenase, beta-D-glucose:NAD(P)+ 1-oxido-reductase, beta-D-glucose:NAD(P)+ 1-oxidoreductase, BmGlcDH-III, BmGlcDH-IV, D-glucose dehydrogenase (NAD(P)), EC 1.1.5.2, GDH, gdh-2, general stress protein 74, GlcDH, GlcDH-I, GlcDH-II, GlcDH-IWG3, glucose 1-dehydrogenase, glucose 1-dehydrogenase 3, glucose 1-dehydrogenase III, glucose 1-dehydrogenase IV, glucose dehydrogenase, glucose1-dehydrogenase, GSP74, hexose phosphate dehydrogenase, hexose-6-phosphate dehydrogenase, LsGDH, More, NAD(P)-dependent glucose-1-dehydrogenase, NAD-GDH, nicotinamide adenine dinucleotide (NAD)-linked glucose dehydrogenase, SsGDH, SSO3204

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.47 glucose 1-dehydrogenase [NAD(P)+]

Temperature Stability

Temperature Stability on EC 1.1.1.47 - glucose 1-dehydrogenase [NAD(P)+]

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 35
stable for 1 week at 30C (pH 6.5), completely inactivated in 30 min at 55C, limited protection by glycerol, 84% activity after 30 min at 65C in the presence of 2 M NaCl,
30
-
20 min, isoenzyme GlcDH-III, without NaCl, about 55% loss of activity
50.2
-
wild-type, melting temperature
52.9
-
mutant L252Q, melting temperature
63.2
-
mutant E170K, melting temperature
66
-
half-life: mutant Q252L 1.3 min, mutant Q252L/E170K 540 min
67.5
-
mutant Q252L, melting temperature
67.6
-
mutant K252Q, melting temperature
70
-
20 min, presence of 2 mM NaCl, complete inactivation of isoenzyme GlcDH-III and GlcDH-IV, isoenzyme GlcDH-IWG3 loses 30% of initial activity
70.3
-
wild-type, melting temperature
72.1
-
mutant F155Y, melting temperature
72.2
-
wild-type, melting temperature
75
-
half-life: approximately 3 h
75.9
-
mutant E170L/K252L, melting temperature
76.1
-
mutant E170K, melting temperature
76.7
-
mutant F155Y, melting temperature
79.2
-
mutant K252L, melting temperature
80
-
15 min, more than 90% remaining activity, pH 8.0
84.9
-
mutant E170K/Q252L, melting temperature
90.7
-
mutant E170K, melting temperature
103
-
half-life: 10 min
additional information