1.1.1.47: glucose 1-dehydrogenase [NAD(P)+]

This is an abbreviated version, for detailed information about glucose 1-dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

D-glucose
+
NAD(P)+
=
D-glucono-1,5-lactone
+
NAD(P)H
+
H+

Synonyms

alkali-resistant glucose 1-dehydrogenase, beta-D-glucose:NAD(P)+ 1-oxido-reductase, beta-D-glucose:NAD(P)+ 1-oxidoreductase, BmGlcDH-III, BmGlcDH-IV, D-glucose dehydrogenase (NAD(P)), EC 1.1.5.2, GDH, gdh-2, general stress protein 74, GlcDH, GlcDH-I, GlcDH-II, GlcDH-IWG3, glucose 1-dehydrogenase, glucose 1-dehydrogenase 3, glucose 1-dehydrogenase III, glucose 1-dehydrogenase IV, glucose dehydrogenase, glucose1-dehydrogenase, GSP74, hexose phosphate dehydrogenase, hexose-6-phosphate dehydrogenase, LsGDH, More, NAD(P)-dependent glucose-1-dehydrogenase, NAD-GDH, nicotinamide adenine dinucleotide (NAD)-linked glucose dehydrogenase, SsGDH, SSO3204

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.47 glucose 1-dehydrogenase [NAD(P)+]

Crystallization

Crystallization on EC 1.1.1.47 - glucose 1-dehydrogenase [NAD(P)+]

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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1.7 A resoluton, four subunits are interrelated by three mutually perpendicular diad axes P,Q and R; micro-seedingtechnique using PEG 2000 as a precipitant
-
batch method using ammonium sulfate as precipitant at pH 6.5 and microdialysis technique with 60% ammonium sulfate in 334 mM NAD, pH 5.8-6.8
-
hanging drop vapour diffusion method with 28% (w/v) PEG 2000, 4 mM NAD+, 40 mM sodium phosphate pH 6.0
-
the crystal structures of BmGlcDH-IV in ligand-free, NADH-bound and beta-D-glucose-bound forms is determined to a resolution of 2.0 A; the crystal structures of BmGlcDH-IV in ligand-free, NADH-bound and beta-D-glucose-bound forms is determined to a resolution of 2.0 A
precipitate collected by centrifugation and dissolved in a minimal volume of 0.1 M potassium phosphate buffer, pH 7.5, 1 mM 2-mercaptoethanol
-
hanging-drop vapour diffusion
-
hanging-drop vapour diffusion using a mother liquor of 12% (v/v) polyethylene glycol 4000, 0.1 M MES (pH 5.8), and 3% (v/v) propan-2-ol
-
precipitate is dissolved in deionized water and ammonium sulfate added to 23% saturation
-
2.9 A resolution, monomer comprises a central nucleotide-binding domain
-
hanging-drop vapour diffusion, 2% polyethylene glycol, pH 5.3, 0.4 mM NADP+, 3.0 A resolution, space group P6122
-
space group P21, the asymmetric unit contains two monomers
-