BRENDA - Enzyme Database show
show all sequences of 1.1.1.47

Significantly enhanced stability of glucose dehydrogenase by directed evolution

Baik, S.H.; Ide, T.; Yoshida, H.; Kagami, O.; Harayama, S.; Appl. Microbiol. Biotechnol. 61, 329-335 (2003)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
usage for quantitative determination of glucose in clinical tests and in the food industry
Bacillus megaterium
diagnostics
usage for quantitative determination of glucose in clinical tests and in the food industry
Bacillus megaterium
synthesis
usage as NADP+ cofactor regenerator for enzymatic synthesis of chiral compounds such as ethyl-(S)-4-chloro-3-hydroxybutanoate and ethyl 4-chloro-3-oxobutanoate
Bacillus megaterium
Cloned(Commentary)
Commentary
Organism
DNA sequence determination and analysis, expression in Escherichia coli strain JM109
Bacillus licheniformis
DNA sequence determination and analysis, expression in Escherichia coli strain JM109
Bacillus megaterium
DNA sequence determination and analysis, expression in Escherichia coli strain JM109
Bacillus subtilis
Engineering
Amino acid exchange
Commentary
Organism
Q252L
natural mutant strain IWG3, Leu252 increases enzyme stability, slightly increased activity compared to the wild-type enzyme
Bacillus megaterium
Q252L/E170K
site-directed mutagenesis of the mutant strain IWG3, the mutant is insensitive against NaCl concentration and pH value, slightly increased activity compared to the wild-type enzyme
Bacillus megaterium
General Stability
General Stability
Organism
NaCl stabilizes the enzyme at alkline pH and elevated temperatures
Bacillus megaterium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
8
-
beta-D-glucose
mutant Q252L, pH 8.0, 25C
Bacillus megaterium
8.5
-
beta-D-glucose
mutant Q252L/E170K, pH 8.0, 25C
Bacillus megaterium
14
-
beta-D-glucose
wild-type enzyme, pH 8.0, 25C
Bacillus megaterium
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
NaCl
stabilizes the enzyme at alkline pH and elevated temperatures
Bacillus megaterium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28200
-
4 * 28200
Bacillus megaterium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus licheniformis
-
IFO 12200
-
Bacillus megaterium
-
mutant strain IWG3, wild-type strain IFO 15308
-
Bacillus subtilis
-
IFO 13719
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli
Bacillus licheniformis
recombinant enzyme from Escherichia coli
Bacillus subtilis
recombinant wild-type and mutant enzymes from Escherichia coli
Bacillus megaterium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-D-glucose + NAD(P)+
best substrate, wild-type and mutant enzymes
654301
Bacillus megaterium
D-glucono-1,5-lactone + NAD(P)H + H+
-
-
-
?
beta-D-glucose + NAD+
-
654301
Bacillus subtilis
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucose + NAD+
-
654301
Bacillus licheniformis
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
D-fructose + NAD(P)+
no activity with mutants Q252L and Q252L/E170K
654301
Bacillus megaterium
? + NAD(P)H
-
-
-
?
D-galactose + NAD(P)+
low activity with wild-type and mutant enzymes
654301
Bacillus megaterium
D-galactono-1,5-lactone + NAD(P)H
-
-
-
?
D-maltose + NAD(P)+
no activity with mutants Q252L and Q252L/E170K
654301
Bacillus megaterium
? + NAD(P)H
-
-
-
?
D-mannose + NAD(P)+
wild-type and mutant enzymes
654301
Bacillus megaterium
? + NAD(P)H
-
-
-
?
D-xylose + NAD(P)+
wild-type and mutant enzymes
654301
Bacillus megaterium
D-xylono-1,5-lactone + NAD(P)H + H+
-
-
-
?
additional information
no activity of wild-type and mutant enzymes with sucrose
654301
Bacillus megaterium
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
More
reversible dissociation in to inactive monomers at alkaline pH
Bacillus megaterium
tetramer
4 * 28200
Bacillus megaterium
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Bacillus licheniformis
25
-
assay at
Bacillus megaterium
25
-
assay at
Bacillus subtilis
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
thermostability is highly increased by addition of NaCl
Bacillus megaterium
66
-
half-life: mutant Q252L 1.3 min, mutant Q252L/E170K 540 min
Bacillus megaterium
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
317
-
beta-D-glucose
mutant Q252L, pH 8.0, 25C
Bacillus megaterium
334
-
beta-D-glucose
mutant Q252L/E170K, pH 8.0, 25C
Bacillus megaterium
395
-
beta-D-glucose
wild-type enzyme, pH 8.0, 25C
Bacillus megaterium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus licheniformis
8
-
assay at
Bacillus megaterium
8
-
assay at
Bacillus subtilis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
sharp drop in activity at alkaline pH of wild-type and mutant Q252L activities, not mutant Q252L/E170K
Bacillus megaterium
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Bacillus licheniformis
NAD+
-
Bacillus subtilis
NAD+
-
Bacillus megaterium
NADP+
-
Bacillus megaterium
Application (protein specific)
Application
Commentary
Organism
analysis
usage for quantitative determination of glucose in clinical tests and in the food industry
Bacillus megaterium
diagnostics
usage for quantitative determination of glucose in clinical tests and in the food industry
Bacillus megaterium
synthesis
usage as NADP+ cofactor regenerator for enzymatic synthesis of chiral compounds such as ethyl-(S)-4-chloro-3-hydroxybutanoate and ethyl 4-chloro-3-oxobutanoate
Bacillus megaterium
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA sequence determination and analysis, expression in Escherichia coli strain JM109
Bacillus licheniformis
DNA sequence determination and analysis, expression in Escherichia coli strain JM109
Bacillus megaterium
DNA sequence determination and analysis, expression in Escherichia coli strain JM109
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Bacillus licheniformis
NAD+
-
Bacillus subtilis
NAD+
-
Bacillus megaterium
NADP+
-
Bacillus megaterium
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
Q252L
natural mutant strain IWG3, Leu252 increases enzyme stability, slightly increased activity compared to the wild-type enzyme
Bacillus megaterium
Q252L/E170K
site-directed mutagenesis of the mutant strain IWG3, the mutant is insensitive against NaCl concentration and pH value, slightly increased activity compared to the wild-type enzyme
Bacillus megaterium
General Stability (protein specific)
General Stability
Organism
NaCl stabilizes the enzyme at alkline pH and elevated temperatures
Bacillus megaterium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
8
-
beta-D-glucose
mutant Q252L, pH 8.0, 25C
Bacillus megaterium
8.5
-
beta-D-glucose
mutant Q252L/E170K, pH 8.0, 25C
Bacillus megaterium
14
-
beta-D-glucose
wild-type enzyme, pH 8.0, 25C
Bacillus megaterium
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
NaCl
stabilizes the enzyme at alkline pH and elevated temperatures
Bacillus megaterium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28200
-
4 * 28200
Bacillus megaterium
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli
Bacillus licheniformis
recombinant enzyme from Escherichia coli
Bacillus subtilis
recombinant wild-type and mutant enzymes from Escherichia coli
Bacillus megaterium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-D-glucose + NAD(P)+
best substrate, wild-type and mutant enzymes
654301
Bacillus megaterium
D-glucono-1,5-lactone + NAD(P)H + H+
-
-
-
?
beta-D-glucose + NAD+
-
654301
Bacillus subtilis
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
beta-D-glucose + NAD+
-
654301
Bacillus licheniformis
D-glucono-1,5-lactone + NADH + H+
-
-
-
?
D-fructose + NAD(P)+
no activity with mutants Q252L and Q252L/E170K
654301
Bacillus megaterium
? + NAD(P)H
-
-
-
?
D-galactose + NAD(P)+
low activity with wild-type and mutant enzymes
654301
Bacillus megaterium
D-galactono-1,5-lactone + NAD(P)H
-
-
-
?
D-maltose + NAD(P)+
no activity with mutants Q252L and Q252L/E170K
654301
Bacillus megaterium
? + NAD(P)H
-
-
-
?
D-mannose + NAD(P)+
wild-type and mutant enzymes
654301
Bacillus megaterium
? + NAD(P)H
-
-
-
?
D-xylose + NAD(P)+
wild-type and mutant enzymes
654301
Bacillus megaterium
D-xylono-1,5-lactone + NAD(P)H + H+
-
-
-
?
additional information
no activity of wild-type and mutant enzymes with sucrose
654301
Bacillus megaterium
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
More
reversible dissociation in to inactive monomers at alkaline pH
Bacillus megaterium
tetramer
4 * 28200
Bacillus megaterium
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Bacillus licheniformis
25
-
assay at
Bacillus megaterium
25
-
assay at
Bacillus subtilis
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
thermostability is highly increased by addition of NaCl
Bacillus megaterium
66
-
half-life: mutant Q252L 1.3 min, mutant Q252L/E170K 540 min
Bacillus megaterium
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
317
-
beta-D-glucose
mutant Q252L, pH 8.0, 25C
Bacillus megaterium
334
-
beta-D-glucose
mutant Q252L/E170K, pH 8.0, 25C
Bacillus megaterium
395
-
beta-D-glucose
wild-type enzyme, pH 8.0, 25C
Bacillus megaterium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus licheniformis
8
-
assay at
Bacillus megaterium
8
-
assay at
Bacillus subtilis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
sharp drop in activity at alkaline pH of wild-type and mutant Q252L activities, not mutant Q252L/E170K
Bacillus megaterium
Other publictions for EC 1.1.1.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724574
Yang
Construction of an integrated ...
Lysinibacillus sphaericus
Biores. Technol.
130
517-521
2013
-
1
1
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-
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-
-
-
-
-
-
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1
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1
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1
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1
1
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1
-
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1
-
-
-
-
-
-
-
-
-
724972
Nishioka
Structure-guided mutagenesis f ...
Bacillus megaterium
FEBS J.
279
3264-3275
2012
-
-
1
1
6
-
-
14
-
-
-
-
-
3
-
-
-
-
-
-
-
-
10
-
1
-
1
-
-
1
-
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-
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2
-
2
6
-
-
-
-
14
-
-
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-
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-
-
-
-
-
-
-
10
-
2
-
2
-
-
2
-
-
-
-
-
-
-
-
718811
Haferkamp
An additional glucose dehydrog ...
Sulfolobus solfataricus
Biochem. Soc. Trans.
39
77-81
2011
-
-
1
-
-
-
2
2
-
-
-
2
-
1
-
-
1
-
-
-
-
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6
-
1
-
-
2
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
2
-
2
-
-
-
2
-
-
-
1
-
-
-
-
6
-
1
-
-
2
1
-
-
-
-
1
1
-
2
2
734842
Haferkamp
-
Biochemical studies of enzymes ...
Sulfolobus solfataricus, Sulfolobus solfataricus DSM 1617
PH. D. Thesis Universitt Duisburg-Essen
2011
0000
2011
-
-
1
-
-
-
-
7
-
-
2
-
-
2
-
-
-
-
-
-
-
-
8
1
-
-
-
8
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
-
-
8
1
-
-
-
8
-
-
-
-
-
-
-
-
8
8
711346
Senesi
Hexose-6-phosphate dehydrogena ...
Homo sapiens, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
Biol. Chem.
391
1-8
2010
-
-
-
-
-
-
-
3
4
-
2
-
-
5
-
1
-
-
-
4
-
-
4
4
-
-
-
-
-
-
-
-
-
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-
-
-
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-
-
-
-
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3
4
-
2
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1
-
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4
-
-
4
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711501
Ding
Cloning and expression in E. c ...
Lysinibacillus sphaericus, Lysinibacillus sphaericus G10
Biores. Technol.
102
1528-1536
2010
1
-
1
-
-
-
8
4
-
13
3
-
8
4
-
-
1
-
-
-
-
2
14
1
1
-
1
3
1
1
3
-
-
-
-
1
-
1
-
-
-
-
-
8
-
4
-
13
3
-
8
-
-
1
-
-
-
2
14
1
1
-
1
3
1
1
3
-
-
-
-
-
-
-
711897
Alcina
Hexose-6-phosphate dehydrogena ...
Homo sapiens
Eur. J. Hum. Genet.
18
618-620
2010
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
713490
Rogoff
Contribution of hexose-6-phosp ...
Mus musculus
Redox Rep.
15
64-70
2010
-
-
-
-
-
-
-
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1
-
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-
2
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1
1
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725684
Park
Enantioselective bioconversion ...
Bacillus subtilis
J. Microbiol. Biotechnol.
20
1300-6
2010
-
1
1
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1
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1
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1
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1
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1
1
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-
1
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1
-
-
-
1
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-
-
-
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-
-
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-
712633
Gomez-Sanchez
Immunohistochemical demonstrat ...
Rattus norvegicus
J. Histochem. Cytochem.
57
633-641
2009
-
-
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-
-
-
-
-
1
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1
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2
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3
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1
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1
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1
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3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684413
Muratsubaki
An enzyme cycling method for m ...
Bacillus megaterium
Anal. Biochem.
378
65-70
2008
-
1
-
-
-
-
-
-
-
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2
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1
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-
684178
Kanaujia
Preliminary X-ray crystallogra ...
Thermus thermophilus HB8
Acta Crystallogr. Sect. F
63
446-448
2007
-
-
1
1
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1
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1
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1
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686083
Vazquez-Figueroa
Development of a thermostable ...
Bacillus licheniformis, Bacillus subtilis subsp. subtilis 168, Bacillus subtilis subsp. subtilis, Bacillus thuringiensis
ChemBioChem
8
2295-2301
2007
-
-
-
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42
-
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-
-
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5
-
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17
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42
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17
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688166
Xu
High-level expression of recom ...
Bacillus megaterium, Bacillus megaterium AS1.223
J. Ind. Microbiol. Biotechnol.
34
83-90
2007
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1
1
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668349
Luna
Glucose metabolism in batch an ...
Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus PAL 3
Curr. Microbiol.
52
163-168
2006
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-
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2
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4
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7
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7
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4
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669452
Milburn
The structural basis of substr ...
Sulfolobus solfataricus
J. Biol. Chem.
281
14796-14804
2006
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1
1
2
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18
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1
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1
2
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18
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4
1
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18
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728653
Britton
Analysis of protein solvent in ...
Haloferax mediterranei, Haloferax mediterranei DSM 1411
Proc. Natl. Acad. Sci. USA
103
4846-4851
2006
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5
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667087
Theodossis
Preliminary crystallographic s ...
Sulfolobus solfataricus
Acta Crystallogr. Sect. F
F61
112-115
2005
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1
1
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1
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668064
Yun
Stereospecific synthesis of (R ...
Bacillus subtilis
Biotechnol. Prog.
21
366-371
2005
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1
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668130
Sharma
Substrate specificity of gluco ...
Enterobacter asburiae, Enterobacter asburiae PSI3
Can. J. Microbiol.
51
477-482
2005
-
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14
-
13
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677657
Baik
Cooperative effect of two surf ...
Bacillus megaterium, Bacillus megaterium IGW3
Appl. Environ. Microbiol.
71
3285-3293
2005
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1
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3
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8
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2
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1
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1
1
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4
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1
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654400
Pire
-
Stability and enzymatic studie ...
Haloferax mediterranei
Biocatal. Biotransform.
22
17-23
2004
1
1
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1
1
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1
3
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2
1
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1
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1
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1
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654301
Baik
Significantly enhanced stabili ...
Bacillus licheniformis, Bacillus megaterium, Bacillus subtilis
Appl. Microbiol. Biotechnol.
61
329-335
2003
-
3
3
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2
1
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3
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1
1
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3
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3
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9
2
3
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3
3
1
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4
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3
4
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2
1
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1
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9
2
3
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2
3
3
1
-
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654857
Inose
Cloning and expression of the ...
Burkholderia cepacia
Biochim. Biophys. Acta
1645
133-138
2003
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1
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1
1
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1
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5
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5
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1
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1
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3
1
1
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2
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1
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656156
Lamble
Metabolic pathway promiscuity ...
Sulfolobus solfataricus
J. Biol. Chem.
278
34066-34072
2003
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1
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1
4
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2
1
1
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1
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1
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12
1
1
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4
1
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3
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1
3
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1
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4
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1
1
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12
1
1
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4
1
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-
-
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-
656567
Ohshima
-
The Sulfolobus tokodaii gene S ...
Sulfolobus tokodaii, Sulfolobus tokodaii 7
J. Mol. Catal. B
23
281-289
2003
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3
1
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9
1
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1
2
2
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12
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1
1
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1
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16
1
1
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2
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1
1
1
4
-
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3
1
4
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9
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1
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1
2
2
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1
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1
-
16
1
1
-
2
-
1
1
1
-
-
-
-
-
-
-
639095
Yamamoto
Crystal structure of glucose d ...
Bacillus megaterium, Bacillus megaterium IWG3
J. Biochem.
129
303-312
2001
-
-
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1
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2
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9
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2
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2
1
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-
1
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-
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-
-
677305
Yamamoto
Crystallization and preliminar ...
Bacillus megaterium, Bacillus megaterium IWG3
Acta Crystallogr. Sect. D
56
1443-1445
2000
-
-
1
1
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8
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2
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2
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2
1
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2
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-
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-
639096
Bhaumik
Kinetic mechanism of glucose d ...
Halobacterium salinarum
Indian J. Biochem. Biophys.
36
143-149
1999
-
-
-
-
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3
4
-
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2
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1
1
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1
1
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1
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1
4
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1
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-
1
-
-
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-
-
-
-
-
-
-
-
285784
Kataoka
Escherichia coli transformant ...
Bacillus megaterium, Bacillus megaterium IWG3
Biosci. Biotechnol. Biochem.
62
167-169
1998
-
-
1
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8
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2
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-
-
-
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-
639097
Siebers
Carbohydrate metabolism in the ...
Thermoproteus tenax
Arch. Microbiol.
168
120-127
1997
1
-
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-
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-
4
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2
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1
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1
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1
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3
1
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1
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1
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4
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1
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1
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3
1
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-
1
-
-
-
-
-
-
-
-
-
-
-
639099
John
-
The crystal structure of gluco ...
Thermoplasma acidophilum
Structure
15
385-393
1994
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639100
Tranulis
Glucose dehydrogenase in beef ...
Bos taurus, Oncorhynchus mykiss
Comp. Biochem. Physiol. B
109
427-435
1994
-
-
-
-
-
-
-
4
2
-
3
-
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5
-
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2
-
-
4
2
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6
1
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9
-
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4
2
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3
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2
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4
2
-
6
1
-
-
-
9
-
-
-
-
-
-
-
-
-
-
639101
Nagao
Cloning, nucleotide sequences, ...
Bacillus megaterium
J. Bacteriol.
174
5013-5020
1992
-
-
1
-
-
1
-
16
-
-
3
-
-
4
-
-
-
-
-
-
-
1
9
1
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-
5
7
-
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5
2
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-
1
2
-
-
1
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-
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16
-
-
3
-
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-
-
-
-
1
9
1
-
-
5
7
-
-
5
-
-
-
-
-
-
-
639102
Bright
-
Crystallization and preliminar ...
Thermoplasma acidophilum
J. Mol. Biol.
22
143-144
1991
-
-
-
1
-
-
-
-
-
-
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-
1
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-
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-
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-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639103
Hilt
Glucose dehydrogenase from Bac ...
Bacillus subtilis
Biochim. Biophys. Acta
1076
298-304
1991
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
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-
1
1
1
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-
-
-
-
1
-
2
1
-
-
-
-
-
1
1
-
-
-
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2
-
-
-
-
-
-
-
1
-
-
1
1
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
639104
Persson
Purification and site-specific ...
Bacillus subtilis
FEBS Lett.
270
41-44
1990
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
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-
1
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-
1
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-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
1
-
-
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1
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-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
639105
Yamamot
Characterization of mutant glu ...
Bacillus megaterium
Ann. N. Y. Acad. Sci.
613
362-365
1990
-
-
1
-
5
-
-
24
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
5
22
-
-
5
2
-
-
-
-
-
1
2
-
5
-
-
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
5
22
-
-
5
-
-
-
-
-
-
-
639106
Smith
Purification and characterizat ...
Thermoplasma acidophilum
Biochem. J.
261
973-977
1989
-
-
-
-
-
1
-
2
-
-
2
-
3
1
-
-
1
-
-
-
1
-
2
1
-
-
2
-
-
-
-
2
-
-
-
-
-
-
2
-
-
1
-
-
-
2
-
-
2
-
3
-
-
1
-
-
1
-
2
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
679615
Mitamura
Enzymatic properties of isozym ...
Bacillus megaterium, Bacillus megaterium IAM 1030, Bacillus megaterium IWG3
Eur. J. Biochem.
186
389-393
1989
-
-
1
-
-
-
-
-
-
-
2
-
-
14
-
-
1
-
-
-
-
-
39
-
-
-
1
2
1
-
-
1
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
6
-
-
-
-
3
-
-
-
-
39
-
-
-
3
6
3
-
-
-
-
-
-
-
-
-
680592
Makino
Stability-increasing mutants o ...
Bacillus megaterium, Bacillus megaterium IWG3
J. Biol. Chem.
264
6381-6385
1989
-
-
1
-
8
-
-
-
-
-
1
-
-
8
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
8
-
-
-
-
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-
1
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639107
Carper
Affinity chromatography of glu ...
Sus scrofa
Experientia
44
29-32
1988
-
-
-
-
-
-
-
4
1
-
-
-
-
1
-
-
1
-
-
1
1
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
4
1
-
-
-
-
-
-
1
-
1
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
679613
Heilmann
Identification and isolation o ...
Bacillus megaterium, Bacillus megaterium M1286
Eur. J. Biochem.
174
485-490
1988
-
-
1
-
-
-
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10
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2
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1
2
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10
-
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-
639108
Maurer
Reversible pH-induced dissocia ...
Bacillus megaterium
Z. Naturforsch. C
42
907-915
1987
-
-
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1
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1
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2
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2
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2
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1
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2
2
-
-
-
-
-
-
2
-
-
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-
-
-
-
639109
Hnes
An integrated prediction of se ...
Bacillus megaterium
FEBS Lett.
212
193-198
1987
-
-
-
-
-
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-
2
-
-
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1
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2
-
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-
-
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-
-
-
679611
Pal
Crystallization of and X-ray i ...
Bacillus megaterium, Bacillus megaterium MI286
Eur. J. Biochem.
167
123-124
1987
-
-
-
1
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1
2
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2
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1
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1
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2
1
-
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-
639110
Giardina
Glucose dehydrogenase from the ...
Sulfolobus solfataricus
Biochem. J.
239
517-522
1986
-
-
-
-
-
4
4
7
-
4
3
-
3
1
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-
1
-
-
-
1
1
21
1
1
1
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-
1
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2
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2
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4
-
4
-
7
-
4
3
-
3
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1
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1
1
21
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
639111
Juhasz
-
The pyridine nucleotide-depend ...
Nostoc sp., Nostoc sp. MAC
FEBS Lett.
194
121-125
1986
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-
-
-
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1
4
-
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4
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1
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1
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4
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2
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2
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1
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4
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1
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1
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4
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639112
Maurer
-
Reversible pH-induced dissocia ...
Bacillus megaterium
Biochim. Biophys. Acta
827
381-388
1985
-
-
-
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1
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1
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1
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1
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1
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1
-
-
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-
1
-
-
-
-
-
-
-
679608
Froeschle
Tyrosine modification of gluco ...
Bacillus megaterium, Bacillus megaterium IWG3
Eur. J. Biochem.
142
533-540
1984
-
-
-
-
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1
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1
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8
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1
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2
1
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1
2
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2
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1
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1
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1
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2
1
-
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-
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-
1
-
-
-
-
-
-
-
639113
Strauss
-
Role of glucose dehydrogenase ...
Bacillus subtilis, Bacillus subtilis NS168I-2
FEMS Microbiol. Lett.
20
379-384
1983
-
-
-
-
-
-
-
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2
-
2
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1
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4
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1
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1
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2
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1
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-
4
-
-
-
-
-
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-
-
-
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-
-
-
-
639114
Carper
Characterization studies of gl ...
Sus scrofa
Experientia
39
1295-1297
1983
-
-
-
-
-
-
-
12
1
-
1
-
-
1
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1
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1
1
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4
1
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1
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1
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1
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12
1
-
1
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1
-
1
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
639115
Ramaley
Glycerol protection and purifi ...
Bacillus subtilis
J. Biol. Chem.
258
12558-12565
1983
-
-
-
-
-
1
-
3
-
-
2
-
-
2
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1
1
1
3
1
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1
2
-
1
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2
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-
1
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3
-
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2
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-
1
1
1
3
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
639116
Thompson
Guanidinium- and temperature-i ...
Sus scrofa
Biochim. Biophys. Acta
745
279-284
1983
-
-
-
-
-
1
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-
1
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1
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1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679607
Ulmer
Evidence for an essential hist ...
Bacillus megaterium, Bacillus megaterium IWG3
Eur. J. Biochem.
136
183-194
1983
-
-
-
-
-
-
1
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8
-
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2
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2
1
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2
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1
1
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639117
Adachi
-
D-glucose dehydrogenase from G ...
Gluconobacter oxydans
Methods Enzymol.
89
159-163
1982
-
-
-
1
-
-
4
2
-
-
2
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-
1
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-
1
-
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-
1
1
1
1
1
-
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-
1
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2
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2
1
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4
-
2
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2
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-
1
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1
1
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
639118
Kobayashi
-
Comparative studies of NAD+-de ...
Corynebacterium sp., Corynebacterium sp. 150-1, Corynebacterium sp. 93-1
Agric. Biol. Chem.
46
2139-2142
1982
-
-
-
-
-
-
-
10
-
-
4
-
-
5
-
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-
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15
1
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2
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-
2
-
2
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2
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10
-
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4
-
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-
-
-
15
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
639119
Campbell
Bovine liver glucose dehydroge ...
Bos taurus
Arch. Biochem. Biophys.
215
289-301
1982
-
-
-
-
-
-
-
5
1
-
2
1
-
1
-
1
1
1
-
1
1
-
3
1
-
-
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-
1
1
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-
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-
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-
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-
5
1
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2
1
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1
1
-
1
1
-
3
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
285782
Adachi
-
Crystallization and characteri ...
Gluconobacter oxydans
Agric. Biol. Chem.
44
301-308
1980
-
-
-
1
-
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4
2
1
-
2
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-
1
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1
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-
1
1
3
1
1
-
-
-
1
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2
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2
1
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4
-
2
1
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2
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-
1
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1
1
3
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
639120
Kobayashi
-
NAD-dependent maltose dehydrog ...
Corynebacterium sp., Corynebacterium sp. 150-1
Agric. Biol. Chem.
44
2837-2841
1980
-
-
-
-
-
-
-
-
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3
-
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2
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1
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1
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1
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-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
639121
Kobayashi
-
Purification and properties of ...
Corynebacterium sp., Corynebacterium sp. 93-1
Agric. Biol. Chem.
44
2261-2269
1980
-
-
-
-
-
-
18
5
-
-
2
-
-
3
-
-
1
-
-
-
1
1
12
1
1
1
2
-
1
1
1
2
1
1
-
-
-
-
2
-
-
-
-
18
1
5
-
-
2
-
-
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-
1
-
-
1
1
12
1
1
1
2
-
1
1
1
1
-
-
-
-
-
-
639123
Yokota
-
Reactivation of inactivated D- ...
Bacillus sp., Bacillus sp. BG 1722
Agric. Biol. Chem.
43
271-278
1979
8
-
-
-
-
1
-
3
-
4
2
-
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2
-
-
1
-
-
-
-
1
10
2
1
-
-
-
1
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-
2
-
-
-
8
-
-
2
-
-
1
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3
-
4
2
-
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-
1
-
-
-
1
10
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
639124
Fujita
Location and properties of glu ...
Bacillus subtilis
J. Bacteriol.
132
282-293
1977
-
-
-
-
-
-
-
5
1
-
1
-
-
2
-
-
1
-
-
1
1
1
3
-
-
-
-
-
1
1
2
1
-
1
-
-
-
-
1
-
-
-
-
-
-
5
1
-
1
-
-
-
-
1
-
1
1
1
3
-
-
-
-
-
1
1
2
1
-
-
-
-
-
-
639125
Pauly
Conformational and functional ...
Bacillus megaterium
Biochemistry
16
4599-4604
1977
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
-
6
-
-
-
-
-
-
-
-
-
-
2
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-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
639126
Pauly
D-Glucose dehydrogenase from B ...
Bacillus megaterium
Hoppe-Seyler's Z. Physiol. Chem.
356
1613-1623
1975
-
-
-
-
-
1
-
2
-
-
2
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1
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1
-
-
-
1
-
4
1
-
-
-
-
2
-
-
2
-
2
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-
-
-
2
-
-
1
-
-
-
2
-
-
2
-
-
-
-
1
-
-
1
-
4
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
639127
Pulich
Pyridine nucleotide-dependent ...
Chlorogloeopsis fritschii, Lyngbya lagerheimii, Nostoc sp., Schizothrix calcicola
J. Biol. Chem.
114
28-33
1973
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
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-
-
-
-
-
6
-
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-
-
-
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-
6
-
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-
-
6
-
-
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-
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-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639128
Shatton
Glucose (hexose 6-phosphate) d ...
Oncorhynchus mykiss
J. Biol. Chem.
246
4878-4885
1971
-
-
-
-
-
-
-
25
-
1
1
-
-
1
-
-
1
-
-
-
1
-
11
-
-
-
2
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
25
-
1
1
-
-
-
-
1
-
-
1
-
11
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-
639129
Thompson
Glucose dehydrogenase from pig ...
Sus scrofa
Biochim. Biophys. Acta
198
397-406
1970
-
-
-
1
-
-
4
2
-
-
-
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-
1
-
-
1
-
-
1
1
-
1
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
4
-
2
-
-
-
-
-
-
-
1
-
1
1
-
1
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-